A Tissue Antigen on the Surface of Cultivated Mouse Epidermal Cells Detected by Mixed Hemadsorption Technique

Mammalian Epidermal Cells in Culture

“…The definite distinction between epithelial and mesenchymal skin cells both in suspensions and monolayer cultures has been possible using epidermal cellspecific antisera directed against tissue-specific membrane antigens which persisted in culture (Worst and Fusenig 1973a;Fusenig and Worst 1975). With the introduction and commercial availability of antikeratin antisera directed against specific keratin polypeptides or against a larger spectrum of different keratins, the unequivocal identification of epidermal (epithelial) cells in culture has become a routine procedure, using either the immunofluorescence or peroxidase technique (e.g., Franke et al 1978;Sun and Green 1978; for further references see Breitkreutz et al 1981Breitkreutz et al , 1984.…”

Demonstration of Epidermis-Specific Heteroantigens in Thymic Epithelial Cells

“…Such heteroantigens have a broader specificity which cuts across species difference. Stratified squamous epithelia of the mouse and rat have heteroantigens which can be detected in the corresponding organ of other species [2,5]. Using heterol ogous anti-guinea pig epidermal cell serum (AES), we found that antigens specific for stratified squamous epithelial cells are also present in thymus epithelial cells but not in thymus lymphocytes.…”