A time to look back: analysis of morphokinetic characteristics of human embryo development

Herrero, Javier; Tejera, A.; Albert, Carmela; Vidal, C.; Santos, María José de los; Meseguer, Marcos

doi:10.1016/j.fertnstert.2013.08.033

Cited by 61 publications

(41 citation statements)

References 30 publications

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“…*P < 0.05, Wilcoxon sum ranked test; **P < 0.001 on day 5 [31]. Several studies have shown a correlation between some morphokinetic parameters and the embryo's implantation potential [18][19][20][21]26]. We now show that the postbiopsy morphokinetics of the biopsied embryos that were implanted (PGD-KID + ) was significantly faster compared to the biopsied PGD-KID − embryos that were not implanted.…”

Section: Discussionmentioning

confidence: 48%

“…During the last 5 years, image capturing with timelapse devices was introduced into in vitro fertilization (IVF) labs, offering a non-invasive method to monitor and analyze the full course of embryo development in real time without interfering with the optimal culture conditions [16,17]. Morphokinetic studies have already shown that embryos with high implantation potential cleave in specific patterns and similar timings [18][19][20][21][22][23]. Those reports demonstrated that morphokinetic evaluation by time-lapse imaging can provide a more comprehensive and detailed analysis of the developmental events than that of static evaluation and thus may assist in selecting top quality embryos for transfer.…”

Section: % Of All Biopsy Procedures Performed In Europe Betweenmentioning

confidence: 99%

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Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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Section: Discussionmentioning

confidence: 48%

Section: % Of All Biopsy Procedures Performed In Europe Betweenmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…However, human data on the exact mechanisms are still lacking. Time-lapse imaging may shed some additional light on the kinetic differences that exist between embryos of normal-weight and overweight/obese women leading to decreased blastocyst formation and implantation rates [27]. A recent study utilizing this technique reported that embryos from overweight/obese women were less likely to reach the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Increased body mass index negatively impacts blastocyst formation rate in normal responders undergoing in vitro fertilization

Comstock

Kim

Behr

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this study is to investigate the effect of female BMI and metabolic dysfunction on blastocyst formation rate. Methods This was a retrospective cohort study that was performed in an academic center for reproductive medicine. Patients who were normal weight, overweight with metabolic dysfunction, or obese who had ≥6 oocytes retrieved in a fresh IVF cycle were included in the study. The blastocyst formation rate was calculated from the number of ≥5 cell embryos on day 3 observed in culture until day 5 or day 6. Only good quality blastocysts were included in the calculation as defined by a morphologic grade of 3BB or better. Results The blastocyst formation rate was significantly better in the normal-weight controls versus overweight/obese patients (57.2 versus 43.6 %, p<0.007). There was no difference in blastocyst formation between the patients with a BMI 25-29.9 kg/m 2 with metabolic dysfunction and those with a BMI ≥30 kg/m 2 . Conclusion The maternal metabolic environment has a significant impact on embryo quality as measured by blastocyst formation. A decreased blastocyst formation rate is likely a significant contributor to poorer reproductive outcomes in overweight and obese women with infertility.

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“…Implanted embryos showed a higher rate for time of t8, tM, tB, and s2 when equated to blastocysts. The results conirmed TLS accuracy for detecting embryo development and implantation potential [11]. Similarly, in an observational study with large sample size, 7483 zygotes inseminated by ICSI were cultured in TLS.…”

Section: Arguments Supporting Predictive Value Of Tlsmentioning

confidence: 52%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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A time to look back: analysis of morphokinetic characteristics of human embryo development

Cited by 61 publications

References 30 publications

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Increased body mass index negatively impacts blastocyst formation rate in normal responders undergoing in vitro fertilization

Embryo Morphokinetics Based on Time-Lapse Observation

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