A time-resolved fluoroimmunoassay for the quantitation of rabies virus nucleoprotein in the rabies vaccine

Lin, Guanfeng; Liu, Tiancai; He, Chunhui; Liu, Jianqing; Chen, Shao-Lang; Hou, Jingyuan; Ren, Zhi-Qi; Wang, Dong; Wu, Yixuan

doi:10.1016/j.jviromet.2014.06.002

Cited by 20 publications

(14 citation statements)

References 28 publications

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“…These features can be utilized for optimization of the measurement conditions to get the maximal sensitivity and to minimize the signal spillover. As the application of TRFIA for quantification of rabies virus nucleoprotein in rabies vaccines was first reported by our research team 24 , TRFIA had been successfully introduced into the rabies vaccine field. As a successful replacement of ELISA, this nucleoprotein TRFIA was highly praised by the vaccine producers.…”

Section: Discussionmentioning

confidence: 97%

“…Monoclonal antibodies against this linear epitope of nucleoprotein have the potential to recognize native rabies virus nucleoprotein 32 , and expression of the nucleoprotein gene of rabies virus can be used as immune antigen and diagnostic reagent 24 , 33 . So the preparation of nucleoprotein MAb will be carried out very smoothly.…”

Section: Discussionmentioning

confidence: 99%

“…Time-resolved fluoroimmunoassay gets excellent precision, higher sensitivity and extremely wider detection range relative to traditional ELISA 27 . Application of TRFIA for detecting viral proteins antigen had been proven to be very applicable by us in the past research 23 , 24 . For acute infectious diseases such as hepatitis B, TRFIA with high sensitivity can assay low level of viral protein in the early stages for disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

Lin

Chen

Zhao

et al. 2017

Sci Rep

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Replacement of the in vivo rabies vaccine potency test (NIH test) by in vitro methods had been discussed by several researcher including WHO expert working groups. In this paper, a time-resolved fluoroimmunoassay (TRFIA) for the assay of rabies virus glycoprotein in rabies vaccine was first established to estimate the rabies vaccine potency by using specific monoclonal antibody that only recognized the native, trimeric and immunogenic form of rabies virus glycoprotein. Potency of the rabies virus glycoprotein was assayed with satisfactory performance under optimal conditions, and the method demonstrated satisfactory results when applied in practical samples. The correlation coefficient of potency values obtained from the present TRFIA and ELISA was 0.912, and 0.903 for those from the present TRFIA and NIH test. These preliminary results confirmed that this TRFIA can replace ELISA with higher performance, and could be a promising replacement of the NIH test. Based upon these results, the present TRFIA seemed to be a convenient tool for evaluating rabies vaccine potency and its products at different stages accordingly.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

Lin

Chen

Zhao

et al. 2017

Sci Rep

Self Cite

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“…Therefore, there is a need for simple, rapid, and inexpensive lead detection methods. The fluorescence immunochromatographic detection technology developed in this study has several advantages of quantitative detection, few false positives, good repeatability, and simple operation (Lin et al, 2014;Majdinasab et al, 2015;Zhang et al, 2015), it has been already developed for the detection of many harmful and toxic substances. (Peng et al, 2011;D.…”

Section: Introductionmentioning

confidence: 99%

Development of a fluorescent quantification strip assay for the detection of lead

Kuang

et al. 2020

Food and Agricultural Immunology

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Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08-1.86 ng/mL, respectively. Additionally, based on this antibody we developed a fluorescent test strip, which can be used for both qualitative and quantitative detection of Pb(II) in brown rice with a detection range of 4.67-47.8 ng/mL. Therefore, the method we developed has high sensitivity suitable for the detection of Pb(II) in brown rice.

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“…However, no report of quantitative AFB1 detection in soybean sauce is available. The introduction of TRFIA in 1980s offered advantages such as rapidity, sensitivity, convenience, and potential for both quantitative and qualitative sensing [ 17 , 18 , 19 ]. Nevertheless, a major concern related to the inherently low fluorescence of Eu(III), which was difficult to measure.…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

Wang

Zhang

et al. 2016

Sensors

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Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg·kg−1, with a limit of detection (LOD) of 0.1 µg·kg−1 and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg−1. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

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A time-resolved fluoroimmunoassay for the quantitation of rabies virus nucleoprotein in the rabies vaccine

Cited by 20 publications

References 28 publications

A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

Development of a fluorescent quantification strip assay for the detection of lead

Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

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