Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

Abstract

Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg·kg−1, with a limit of detection (LOD) of 0.1 µg·kg−1 and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg−1. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

Rapid and sensitive double‐label based immunochromatographic assay for zearalenone detection in cereals

“…Antibody‐EuNP probes were prepared by conjugating the amino groups of the antibody with the carboxyl groups of EuNP. Usually, the activation methods and the EuNP size affected DL‐ICA sensitivity and reproducibility . The EDC/NHS coupling method was used to activate EuNP, resulting in enhanced fluorescence intensity and activation efficiency compared with EDC or NHS coupling method.…”

“…According to a previously published study, the portable reader was modified for quantitative sensing . The excitation light wavelength was 365 nm via a light emitting diode (LED), while signal acquisition wavelength was obtained at 613 ±10 nm using a photomultiplier tube (PMT).…”

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

“…In addition, different matrices are complex because of the coexistence of various nutritious components such as carbohydrates, proteins, minerals, and fats, which cause severe interference during analysis. At present, various procedures for sample clean‐up and/or analyte enrichment have been developed, including SPE, LLE, QuEChERS, immunoaffinity column (IAC), and low temperature cleanup [16–22]. These methods have their own advantages or limitations.…”

An on-site, ultra-sensitive, quantitative sensing method for the determination of total aflatoxin in peanut and rice based on quantum dot nanobeads strip

“…It can also achieve a sensitive, low-cost determination for AFT, thereby guaranteeing the potential on-site application. Typically, the gold nanoparticle has been substantiated as an effective reporter for colorimetric detection, allowing the qualitative determination for mycotoxin with relatively high concentrations [10,11,12,13].Furthermore, to meet the requirements of detection of sensitive mycotoxins such as AFT, some quantitative test strip methods have been developed recently using various reporters, such as liposome-encapsulated dyes [14], magnetic nanogold microspheres [15],time-resolved fluorescence [16,17,18], up-converting phosphor [19], fluorescent microspheres [20], and quantum dots (QDs) [21,22,23].Owing to the intrinsically high sensitivity, the fluorescence-based strip could qualify application in food safety [9].The organic fluorescence could be hampered from the extensive application due to their inherent photobleaching, thus lowering the sensitivity. QDs have been proven as one of the optimum reporters.…”