A Three‐dimensional View of Platelet Responses to Chemical Stimuli*

Barnhart, Marion I.; Walsh, R. T.; Robinson, Jennifer A.

doi:10.1111/j.1749-6632.1972.tb16311.x

Cited by 66 publications

(29 citation statements)

References 24 publications

(5 reference statements)

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“…About 609% of the platelets in the effluent had lost their discoid shape and appeared slightly swollen with ruffling to give a "berrylike" appearance. These surface changes induced by short exposure to collagen resemble those for contact activation by a formvar film surface (38). Examination of the effluent from a BSA column revealed almost the same pattern as the control static platelets (60%6 discoid, 30%o with short pseudopodia; Fig.…”

mentioning

confidence: 78%

High-speed platelet adhesion under conditions of rapid flow.

Polanowska-Grabowska¹,

Gear²

1992

Proc. Natl. Acad. Sci. U.S.A.

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The recognition of exposed collagen by circulating platelets is an initial step in the formation of the hemostatic plug or a thrombus after vascular ijury. Theoretical calculations of the speed of platelet function required for effective hemostasis have suggested very short reaction times. However, it is not known how fast platelets can adhere to collagen under arterial flow conditions or which membrane proteins are involved. We have used a continuous-flow, microaffinity column linked to a resistive-particle counter to detect platelet adhesion. Adhesion of human platelets to native ype I collagen was extremely rapid, with exponential halftimes as short as 240 ms, and was nearly complete by 2 s. This RGD-independent process was not associated with platelet aggregation or secretion. The monoclonal antibody 6F1 directed against the glycoprotein Ia/HIa complex inhibited adhesion, suggesting that this complex plays an important role in the initial phases of platelet-collagen interaction under flow conditions. In addition, divalent cations were required for adhesion, as indicated by inhibition with EDTA in plasma and the dependence on Mg2+ for washed platelets.

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mentioning

confidence: 78%

High-speed platelet adhesion under conditions of rapid flow.

Polanowska-Grabowska¹,

Gear²

1992

Proc. Natl. Acad. Sci. U.S.A.

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“…Well-defined sequence of morphological changes can be classified as (Barnhart et al, 1972;Ko et al, 1993;Goodman et al, 1989): 1-round or discoid, 2-dendritic or early pseudopodial, without flattening, 3-spread-dendritic or intermediate pseudopodial, 4-spread or late pseudopodial, 5-fully spread ( Figure 5). After activation, platelets contract and release the contents of their granules into the extracellular environment.…”

Section: Platelet Adhesionmentioning

confidence: 99%

Haemocompatibility evaluation of DLC- and SiC-coated surfaces

Nurdin¹,

Francois²,

Mugnier³

et al. 2003

eCM

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Diamond-like carbon (DLC) and silicon carbide (SiC) coatings are attractive because of low friction coefficient, high hardness, chemical inertness and smooth finish, which they provide to biomedical devices. Silicon wafers (Si waf ) and silicone rubber (Si rub ) plates were coated using plasmaenhanced chemical vapour deposition (PE-CVD) techniques. This article describes: 1-the characterization of modified surfaces using attenuated total reflection-Fourier transform infrared spectroscopy (ATR/FTIR) and contact angle measurements, 2-the results of three in-vitro haemocompatibility assays. Coated surfaces were compared to uncoated materials and various substrates such as polymethylmethacrylate (PMMA), polyethylene (LDPE), polydimethylsiloxane (PDMS) and medical steel (MS). Thrombin generation, blood platelet adhesion and complement convertase activity tests revealed the following classification, from the most to the least heamocompatible surface: Si rub / DLC-Si rub / DLC-Si waf / LDPE/ PDMS/ SiCSi waf / Si waf / PMMA/ MS. The DLC coating surfaces delayed the clotting time, tended to inhibit the platelet and complement convertase activation, whereas SiC-coated silicon wafer can be considered as thrombogenic. This study has taken into account three events of the blood activation: coagulation, platelet activation and inflammation. The response to those events is an indicator of the in vitro haemocompatibility of the different surfaces and it allows us to select biomaterials for further in vivo blood contacting investigations.

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“…In the case of PRP, both A and k would be expected to undergo complex changes dur ing the course of aggregation. Due to the fact that the particles are morphologically dynamic in time [8,10,11,16], even the definition o f 'size' is vexing. Platelet shape and chemical changes, along with aggregate for mation, can be expected to contribute to variations in the optical quality of the particles.…”

Section: Methodsmentioning

confidence: 99%

“…This is interpreted as the first ('aggregation') phase. Morphological changes occur, including swelling and the appearance of dendrites and pseudopodia [8,9,11]. Aggregation includes platelet-platelet and platelet-aggregate adhe sion, as well as aggregate-aggregate, in which smaller aggregates coalesce into larger aggregates [5,14], As aggregation proceeds, there is a decrease in the total number of individual particles (platelets + aggregates) while the average particle size increases [4,10,11,14], During this latter period, the extinction curve may exhibit a change in slope, interpreted to be indicative of an increase in the rate of aggregation.…”

Section: Methodsmentioning

confidence: 99%

In vitro Aggregation of Normal Platelets: A Hypothetical First-Approximation Model for Light Extinction in Platelet-Rich Plasma

Boudreau¹,

Rock²

1986

Pathophysiol Haemos Thromb

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Aggregometer tracings of light transmission through platelet-rich-plasma are routinely used for the clinical evaluation of platelet function. The analytical relation between the changing aggregometer curve tracings of the light extinction and the aggregation process remains unknown. A first-approximation equation for this relation is proposed, based upon a highly simplified view of the in vitro aggregation process for normal platelets. The model described is capable of generating curves which show strong resemblance to those predicted by light scattering theory as well as those observed experimentally, including biphasic structures.

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A Three‐dimensional View of Platelet Responses to Chemical Stimuli*

Cited by 66 publications

References 24 publications

High-speed platelet adhesion under conditions of rapid flow.

High-speed platelet adhesion under conditions of rapid flow.

Haemocompatibility evaluation of DLC- and SiC-coated surfaces

In vitro Aggregation of Normal Platelets: A Hypothetical First-Approximation Model for Light Extinction in Platelet-Rich Plasma

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