A three-dimensional dynamic coculture system enabling facile cell separation for chondrogenesis of mesenchymal stem cells

Xu, Feiyue; Xu, Lei; Wang, Qi; Zhou, Yan; Ye, Zhaoyang; Tan, Wen‐Song

doi:10.1016/j.bej.2015.07.003

Cited by 6 publications

(6 citation statements)

References 42 publications

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“…Both rbBMSCs and rbACs were isolated from New Zealand white rabbits as described previously [19][20][21]. Bovine ACs (bACs) were isolated similarly from adult bovine stifle joints obtained from a local a commercial vendor.…”

Section: Cellsmentioning

confidence: 99%

Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion

et al. 2017

Tissue Eng Regen Med

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Coculture between mesenchymal stem cells (MSCs) and chondrocytes has significant implications in cartilage regeneration. However, a conclusive understanding remains elusive. Previously, we reported that rabbit bone marrowderived MSCs (rbBMSCs) could downregulate the differentiated phenotype of rabbit articular chondrocytes (rbACs) in a non-contact coculture system for the first time. In the present study, a systemic investigation was performed to understand the biological characteristics of chondrocytes in coculture with MSCs. Firstly, cells (MSCs and chondrocytes) from different origins were cocultured in transwell system. Different chondrocytes, when cocultured with different MSCs respectively, consistently demonstrated stimulated proliferation, transformed morphology and declined glycosaminoglycan secretion of chondrocytes. Next, cell surface molecules and the global gene expression of rbACs were characterized. It was found that cocultured rbACs showed a distinct surface molecule profile and global gene expression compared to both dedifferentiated rbACs and rbBMSCs. In the end, cocultured rbACs were passaged and induced to undergo the chondrogenic redifferentiation. Better growth and chondrogenesis ability were confirmed compared with control cells without coculture. Together, chondrocytes display comprehensive changes in coculture with MSCs and the cocultured rbACs are beneficial for cartilage repair.

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Section: Cellsmentioning

confidence: 99%

Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion

et al. 2017

Tissue Eng Regen Med

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“…Rabbit ACs (rACs) and rabbit MSCs (rMSCs) were isolated as described in our previous work . All animal experiments were performed at Shanghai Laboratory Animal Center (Shanghai) in accordance with the institutional guidelines of animal care and use committee.…”

Section: Methodsmentioning

confidence: 99%

Poly(ε‐caprolactone)‐based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis

Chen

Zhou

et al. 2016

Cell Proliferation

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“…Usually 50% of the culture medium is replaced every two days [12,13] and the procedure induces chondrogenesis in AChs, MSCs, and co-cultures of both [14]. In one study with bovine AChs (bAChs), seeded into polyglycolid acid (PGA) scaffolds, culture results in cartilage constructs with 70% more cells, 60% more glycosaminoglycogen (GAG), and 125% more collagen compared to a petri dish [15].…”

Section: Bioreactors For Articular Cartilage Tissue Engineeringmentioning

confidence: 99%

A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond

Nazempour¹,

Wie²

2018

J Stem Cell Ther Transplant

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A three-dimensional dynamic coculture system enabling facile cell separation for chondrogenesis of mesenchymal stem cells

Cited by 6 publications

References 42 publications

Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion

Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion

Poly(ε‐caprolactone)‐based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis

A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond

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