A three-dimensional cell-loading system using autologous plasma loaded into a porous β-tricalcium-phosphate block promotes bone formation at extraskeletal sites in rats

Tajima, N.; Sotome, Shinichi; Marukawa, Eriko; Omura, Ken; Shinomiya, Kenichi

doi:10.1016/j.msec.2006.05.031

Cited by 23 publications

(20 citation statements)

References 32 publications

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“…Marx et al reported that PRP accelerated the rate and degree of bone formation in a bone graft. However, in our in vitro study (Tajima et al, 2007), when soluble factors from PRP were added to cultures of rat bone marrow stromal cells, they promoted proliferation but inhibited osteoblast differentiation in a dose-dependent manner. This finding agrees with those of other authors (Arpornmaeklong et al, 2004;Soffer et al, 2004;Ogino et al, 2005).…”

Section: Discussionmentioning

confidence: 58%

“…We believe that the fibrin networks of PRP might aid the decrease in postoperative bone resorption. The function of fibrin networks is as an osteoconductive scaffold (Soffer et al, 2003;Le Guehennec et al, 2005;Segura-Castillo et al, 2005;Catelas et al, 2006;Huh et al, 2006;Tajima et al, 2007), and thus the fibrin gel would have provided a matrix for cell growth and differentiation by enhancing three-dimensional intercellular interactions or cell adhesion, both of which are thought to be good environments for the maturation of osteoblasts (Catelas et al, 2006;Tajima et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Reduction of bone resorption by the application of platelet-rich plasma (PRP) in bone grafting of the alveolar cleft

Marukawa

Oshina

Iino

et al. 2011

Journal of Cranio-Maxillofacial Surgery

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Reduction of bone resorption by the application of platelet-rich plasma (PRP) in bone grafting of the alveolar cleft

Marukawa

Oshina

Iino

et al. 2011

Journal of Cranio-Maxillofacial Surgery

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“…[38][39][40][41][42][43][44] We have demonstrated in a previous in vitro study that PRP promotes cell proliferation and inhibits osteoblast differentiation of MSCs in a dose-dependent manner. 36 Furthermore, the results of the in vivo study showed that artificial bone materials enhanced bone formation with a combination of PPP and MSCs, whereas those with a combination of PRP and MSCs had an inhibitory effect on bone formation.…”

Section: Discussionmentioning

confidence: 98%

“…45,46 Findings from our previous study demonstrated that fibrin gels provide a matrix for cell growth and differentiation and provide a preferable environment for osteoblastic differentiation, during which cells contact fibrin molecules and exhibit three-dimensional cell-cell interactions. 36 The fibrin network strength of especially PRF is strong, it be considered that PRF has played a role as space making for bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…We reported that PPP with bone marrow stromal cells (MSCs) and b-TCP scaffolds promoted bone formation to a greater extent than PRP with MSCs did. 36 The amount and rate of bone regeneration depend on the type of material to be used, the size of the socket to be filled, and the condition of the remaining bone walls; therefore, careful consideration should be paid to the use of each material. However, no report has evaluated sockets with buccal dehiscence, which makes preservation of the alveolar ridge difficult.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Platelet-Poor Plasma, Platelet-Rich Plasma, and Platelet-Rich Fibrin on Healing of Extraction Sockets with Buccal Dehiscence in Dogs

Hatakeyama¹,

Marukawa²,

Takahashi³

et al. 2013

Tissue Engineering Part A

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Alveolar bone resorption generally occurs during healing after tooth extraction. This study aimed to evaluate the effects of platelet-poor plasma (PPP), platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) on healing in a ridge-augmentation model of the canine socket with dehiscence of the buccal wall. The third mandibular premolars of 12 beagle dogs were extracted and a 3 mm buccal dehiscence from the alveolar crest to the buccal wall of the extraction socket was created. These sockets were then divided into four groups on the basis of the material used to fill the sockets: PPP, PRP, PRF, and control (no graft material) groups. Results were evaluated at 4 and 8 weeks after surgery. The ultrastructural morphology and constructs of each blood product were studied by a scanning electron microscope (SEM) or calculating concentrations of platelets, fibrinogen, platelet-derived growth factor, and transforming growth factor-β. A total of five microcomputed tomography images of specimens were selected for measurement, and the area occupied by the newly formed bone as well as the horizontal bone width were measured. Moreover, decalcified tissue specimens from each defect were analyzed histologically. The median area of new bone at 4 and 8 weeks and median horizontal bone width at 8 weeks were the highest in the PPP group. However, bone maturation in the PRF and the PRP groups was more progressed than that in the PPP and control groups. By SEM findings, the PRF group showed a more highly condensed fibrin fiber network that was regularly arranged when compared with the PPP and PRP groups. The growth factors released from platelets in PRP indicated higher concentrations than that in PRF. Under more severe conditions for bone formation, as in this experiment, the growth factors released from platelets had a negative effect on bone formation. This study showed that PPP is an effective material for the preservation of sockets with buccal dehiscence.

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Improving bone repair of femoral and radial defects in rabbit by incorporating PRP into PLGA/CPC composite scaffold with unidirectional pore structure

Chen

et al. 2014

J. Biomed. Mater. Res.

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In this study, a platelet-rich plasma poly(lactic-co-glycolic acid) (PRP-PLGA)/calcium phosphate cement (CPC) composite scaffold was prepared by incorporating PRP into PLGA/CPC scaffold with unidirectional pore structure, which was fabricated by the unidirectional freeze casting of CPC slurry and the following infiltration of PLGA. The results from in vitro cell experiments and in vivo implantation in femoral defects manifested that incorporation of PRP into PLGA/CPC scaffold improved in vitro cell response (cell attachment, proliferation, and differentiation), and markedly boosted bone formation, angiogenesis and material degradation. The incorporation of PRP into scaffold showed more outstanding improvement in osteogenesis as the scaffolds were used to repair the segmental radial defects, especially at the early stage. The new bone tissues grew along the unidirectional lamellar pores of scaffold. At 12 weeks postimplantation, the segmental radial defects treated with PRP-PLGA/CPC scaffold had almost recuperated, whereas treated with the scaffold without PRP was far from healed. Taken together, the PRP-PLGA/CPC scaffold with unidirectional pore structure is a promising candidate to repair bone defects at various sites.

show abstract

A three-dimensional cell-loading system using autologous plasma loaded into a porous β-tricalcium-phosphate block promotes bone formation at extraskeletal sites in rats

Cited by 23 publications

References 32 publications

Reduction of bone resorption by the application of platelet-rich plasma (PRP) in bone grafting of the alveolar cleft

Reduction of bone resorption by the application of platelet-rich plasma (PRP) in bone grafting of the alveolar cleft

Effects of Platelet-Poor Plasma, Platelet-Rich Plasma, and Platelet-Rich Fibrin on Healing of Extraction Sockets with Buccal Dehiscence in Dogs

Improving bone repair of femoral and radial defects in rabbit by incorporating PRP into PLGA/CPC composite scaffold with unidirectional pore structure

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