A third human CALC (pseudo)gene on chromosome 11

Höppener, Jo W.M.; Steenbergh, P. H.; Zandberg, J.; Adema, Gosse J.; Kessel, A.H.M. Geurts van; Lips, Cornelis J.M.; Jansz, H.S.

doi:10.1016/0014-5793(88)81355-3

Cited by 20 publications

(5 citation statements)

References 31 publications

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“…The human, mouse and rat calcitonin family includes calcitonin or the calcitonin gene-related peptide 1 ( CGRP-I or alpha-type CGRP or CALCA ) and calcitonin gene-related peptide 2 ( CGRP-II or beta-type CGRP or CALCB ) genes. Alternative splicing of CALCA , a human calcitonin pseudogene ( CALCP ) [ 39 ] and three calcitonin-related proteins in pig [ 40 ] have been reported.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches

2009

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Background: Neuropeptides are cell to cell signalling molecules that regulate many critical biological processes including development, growth and reproduction. These peptides result from the complex processing of prohormone proteins, making their characterization both challenging and resource demanding. In fact, only 42 neuropeptide genes have been empirically confirmed in cattle. Neuropeptide research using high-throughput technologies such as microarray and mass spectrometry require accurate annotation of prohormone genes and products. However, the annotation and associated prediction efforts, when based solely on sequence homology to species with known neuropeptides, can be problematic.

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Section: Resultsmentioning

confidence: 99%

Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches

2009

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“…ctif?& rw&rih< +&&&i,, m+ifg hybrids containing translocation-derived chromosomes, placed the IAPP locus within the ql4-pter region. The gene is thus not located in close proximity to the related CALC-I, CALC-II and CALC-III genes, which were assigned to chromosome 1 I [20,25,26]. This observation implies that the exon 2-and 3-like sequences of CALC-III [26] are not directly related to the IAPP gene.…”

Section: Plaquelifts Northern Blotting Southern Blotting and Hybridmentioning

confidence: 94%

“…The gene is thus not located in close proximity to the related CALC-I, CALC-II and CALC-III genes, which were assigned to chromosome 1 I [20,25,26]. This observation implies that the exon 2-and 3-like sequences of CALC-III [26] are not directly related to the IAPP gene. Probably the IAPP gene arose by duplication and translocation to chromosome 12 of a progenitor CALC-gene and subsequent sequence divergence.…”

Section: Plaquelifts Northern Blotting Southern Blotting and Hybridmentioning

confidence: 94%

Islet amyloid polypeptide: Identification and chromosomal localization of the human gene

et al. 1988

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Islet or insulinoma amyloid polypeptide (IAPP) is a 37 amino acid polypeptide isolated from pancreatic amyloid. Here, we describe the isolation and partial characterization of the human gene encoding IAPP. The DNA sequence predicts that IAPP is excised from a larger precursor protein and that its carboxy-terminus is probably amidated. The predicted normally occurring IAPP is identical to the reported polypeptides isolated from pancreatic amyloid, except for the amidated carboxy-terminus.IAPP specific polyadenylated RNAs of 1.6 kb and 2.1 kb are present in human insulinoma RNA. The human IAPP gene is located on chromosome 12.

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“…CALC II produces CGRP II but not CT because exon 4 contains a stop codon (1). CALC III is a pseudogene (3). Adrenomedullin (ADM) is encoded by CALC IV and amylin by CALC V genes (1).…”

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confidence: 99%

Autocrine/Paracrine Role of Inflammation-Mediated Calcitonin Gene-Related Peptide and Adrenomedullin Expression in Human Adipose Tissue

et al. 2005

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Human adipose tissue is a contributor to inflammation- and sepsis-induced elevation of serum procalcitonin (ProCT). Several calcitonin (CT) peptides, including ProCT, CT gene-related peptide (CGRP), and adrenomedullin (ADM) are suspected mediators in human inflammatory diseases. Therefore, we aimed to explore the expression, interactions, and potential roles of adipocyte-derived CT peptide production. Expression of CT peptide-specific transcripts was analyzed by RT-PCR and quantitative real-time PCR in human adipose tissue biopsies and three different inflammation-challenged human adipocyte models. ProCT, CGRP, and ADM secretions were assessed by immunological methods. Adipocyte transcriptional activity, glycerol release, and insulin-mediated glucose transport were studied after exogenous CGRP and ADM exposure. With the exception of amylin, CT peptides were expressed in adipose tissue biopsies from septic patients, inflammation-activated mature explanted adipocytes, and macrophage-activated preadipocyte-derived adipocytes. ProCT and CGRP productions were significantly augmented in IL-1beta and lipopolysaccharide-challenged mesenchymal stem cell-derived adipocytes but not in undifferentiated mesenchymal stem cells. In contrast, ADM expression occurred before and after adipogenic differentiation. Interferon-gamma coadministration inhibited IL-1beta-mediated ProCT and CGRP secretion by 78 and 34%, respectively but augmented IL-1beta-mediated ADM secretion by 50%. Exogenous CGRP and ADM administration induced CT, CGRP I, and CGRP II mRNAs and dose-dependently (10(-10) and 10(-6) m) enhanced glycerol release. In contrast, no CGRP- and ADM-mediated effects were noted on ADM, TNFalpha, and IL-1beta mRNA abundances. In summary, CGRP and ADM are two differentially regulated novel adipose tissue secretion factors exerting autocrine/paracrine roles. Their lipolytic effect (glycerol release) suggests a metabolic role in adipocytes during inflammation.

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A third human CALC (pseudo)gene on chromosome 11

Cited by 20 publications

References 31 publications

Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches

Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches

Islet amyloid polypeptide: Identification and chromosomal localization of the human gene

Autocrine/Paracrine Role of Inflammation-Mediated Calcitonin Gene-Related Peptide and Adrenomedullin Expression in Human Adipose Tissue

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