2009
DOI: 10.1186/1471-2164-10-228
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Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches

Abstract: Background: Neuropeptides are cell to cell signalling molecules that regulate many critical biological processes including development, growth and reproduction. These peptides result from the complex processing of prohormone proteins, making their characterization both challenging and resource demanding. In fact, only 42 neuropeptide genes have been empirically confirmed in cattle. Neuropeptide research using high-throughput technologies such as microarray and mass spectrometry require accurate annotation of p… Show more

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Cited by 23 publications
(28 citation statements)
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“…[19,50]. The initial list of candidate prohormone genes was derived from known mammalian genes supplemented by known or homologous avian genes identified by Delfino et al .…”
Section: Methodsmentioning
confidence: 99%
“…[19,50]. The initial list of candidate prohormone genes was derived from known mammalian genes supplemented by known or homologous avian genes identified by Delfino et al .…”
Section: Methodsmentioning
confidence: 99%
“…A master list of candidate genes was generated based on known human and chicken prohormone gene sequences available in public databases and a literature review (Amare et al, 2006; Southey et al, 2008; Southey et al, 2009). The human sequences offer a good representation of the mammalian genes (Tegge et al, 2008) and were complemented with already known chicken sequences not detected in mammalian species.…”
Section: Methodsmentioning
confidence: 99%
“…Several models have been proposed to predict the cleavage of prohormone proteins coded by neuropeptide genes (Hummon et al, 2005; Southey et al, 2006b; Tegge et al, 2008; Southey et al 2008; Southey et al 2009). However, no cleavage model has been trained on avian species.…”
Section: Methodsmentioning
confidence: 99%
“…Unlike prototypic tumor suppressor genes (Lee and Muller, 2010; Rothenberg and Settleman, 2010), however, bioinformatic algorithms predict that Ecrg4 is not an intracellular cytoplasmic protein (Mirabeau et al, 2007; Schuster-Bockler and Bateman, 2007; Southey et al, 2009; Tegge et al, 2008). Instead, Ecrg4 resembles a ligand encoding a neuropeptide-like precursor characteristic of the human secretome (Clark et al, 2003; Hathout, 2007; Mustafa et al, 2011).…”
Section: Introductionmentioning
confidence: 99%