A third DNA‐dependent ATPase from Bacillus cereus free of ATP‐dependent DNase activity

Abstract

The purification of ATP‐dependent DNase from Bacillus cereus led to the isolation and characterization of a third DNA‐dependent ATPase. The enzyme called ATPase III has been purified free of nuclease activity. None of the expected ATPases proved to be identical with ATP‐dependent DNase‐DNA‐dependent ATPase. Separation of ATPase I, II and III and a DNase specific for single‐stranded DNA from the same source excludes the possibility of ATP‐dependent DNase being the action of a single enzyme molecule.

Resolution and reconstitution of the rec BC deoxyribonuclease of Escherichia coli

“…Three DNAdependent ATPases have also been found in Ps. aeruginosa [21], B. subtilis [25] and in B. cereus [26]. Two of the B. cereus ATPases were proved to be unwinding enzymes [15,27].…”