A thin-layer and column chromatography experiment adapted for use in secondary schools. A quick, safe, colorful, convenient introduction to two basic techniques

Abstract

The paper is taken from the beaker when the solvent front is about 2 cm from the top. Because the paper remains wet for a long time, the solvent continues forward for several minutes after the paper is removed from the developing solution. For this reason, it is

A Fluorogenic Aromatic Nucleophilic Substitution Reaction for Demonstrating Normal-Phase Chromatography and Isolation of Nitrobenzoxadiazole Chromophores

“…The reaction carried out with ethanolamine can be reduced and purified by flash column chromatography. A disposable “pipet column” is prepared using a small quantity of silica (0.45 g) . The product is followed visually or by long-wavelength UV lamp irradiation (365 nm) by its bright yellow fluorescence (see Figures SI3 and SI4 in the supporting information).…”

Separation and Analysis of Ginsenoside Rg1: An Experiment for the Separation and Analysis of Natural Products

“…Due to their extensive applicability in separating and analyzing active components from natural products, silica gel column chromatography (SGCC), thin layer chromatography (TLC), and high-performance liquid chromatography (HPLC) are the techniques with which undergraduate students studying pharmaceutical engineering should be acquainted. Several reports on these techniques incorporated into undergraduate teaching laboratories have been published in this Journal . − These reports focused mainly on the students’ mastery of these techniques, which was the training of experimental skills, − such as to separate an unknown mixture consisting of two colorless solids, a mixture of vivid colored components, ,, or a specific active ingredient in a natural product, ,,, to help students become familiar with the operation of column chromatography (CC) or to optimize the separation process of CC to help students understand the separation principle of CC in-depth. ,, Only a few reports focused on the overall knowledge structure formed by connecting the fragmented knowledge or skills in teaching. − Fragmented learning is only learning certain knowledge units. Its drawbacks are obvious, such as the fact that the links between the various fragmented knowledge units are scattered and the lack of a mutual logical relationship.…”

Óleo essencial de limão no ensino da cromatografia em camada delgada

“…Diversas experiências para serem utilizadas no ensino da CCD estão descritas na literatura especializada, tanto em livros-texto 1 quanto em artigos de revistas. [2][3][4][5][6][7][8][9] Entretanto, na sua grande maioria, apenas uma variável da técnica é estudada como, por exemplo, comparação de fatores de retenção de substâncias diferentes em um mesmo sistema de eluição, 2,5,8,9 estudo de diferentes sistemas de eluição na separação dos componentes de uma determinada mistura 3,4 ou estudo de sistemas de revelação diferentes de um cromatograma. 6,8 Também estão descritas experiências onde há a articulação da CCD com outras técnicas lecionadas nas disciplinas de química orgânica experimental, tais como cromatografia em coluna 2,9 e extração ácido-base.…”