A Theoretical Model for Immobilized Enzyme Inhibition Biosensors

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

“…The choline is oxidized by ChOx in the presence of oxygen and H 2 O 2 is produced (Ciucu et al, 2003;Lin et al, 2004;Zhang et al, 2001a). Therefore, it is important to improve the sensitivity of H 2 O 2 detection.…”

Enzyme‐Based Biosensors: Synthesis and Applications

“…Among them, the electrochemical amperometric transducer came to be the principal choice due to its high sensitivity and has received great attention [60,168] . Although biosensor systems based on enzyme inhibition through a quantitative measurement of the enzyme activity of an immobilized enzyme before and after exposure to a target analyte are very sensitive and have been extensively used in environmental monitoring, the enzyme -analyte system is carefully selected such that the analyte often may result in the inhibition of normal enzyme function [169] . In addition, inhibition biosensors often present some major limitations like tedious incubation procedures and multiple reaction steps, the permanent nature of the enzyme inhibition leading to irrecoverable after -to -use activity, and poor selectivity [25,156,169] .…”

“…Although biosensor systems based on enzyme inhibition through a quantitative measurement of the enzyme activity of an immobilized enzyme before and after exposure to a target analyte are very sensitive and have been extensively used in environmental monitoring, the enzyme -analyte system is carefully selected such that the analyte often may result in the inhibition of normal enzyme function [169] . In addition, inhibition biosensors often present some major limitations like tedious incubation procedures and multiple reaction steps, the permanent nature of the enzyme inhibition leading to irrecoverable after -to -use activity, and poor selectivity [25,156,169] . These drawbacks can be effectively overcome in catalyticmode biosensors based on the organophosphorus hydrolase enzyme for organophosphorus pesticides, as shown by Rainina et al [170] , Chen -Goodspeed et al [171] , Mulchandani et al [156] , and Grimsley et al [172] .…”

Implementation of the Discrete Wavelet Transform Used in the Calibration of the Enzymatic Biosensors

“…A variety of linear, non-linear, logarithmic responds between inhibition percentage and either inhibition concentration or incubation time were reported (Evtugyn et al, 1999;Fennouh et al, 1997;Hart et al, 1997). Concerning biosensors based on acetylcholinesterase inhibition, a theoretical model was reported based on diffusion limited inhibitor transport, taking into account the heterogeneous nature of enzyme inhibition that results from immobilization at the transducer surface (Zhang et al, 2001) :…”