A Tetra-Panel of Serum Circulating miRNAs for the Diagnosis of the Four Most Prevalent Tumor Types

Abstract

The purpose of this study is to clinically validate a series of circulating miRNAs that distinguish between the 4 most prevalent tumor types (lung cancer (LC); breast cancer (BC); colorectal cancer (CRC); and prostate cancer (PCa)) and healthy donors (HDs). A total of 18 miRNAs and 3 housekeeping miRNA genes were evaluated by qRT-PCR on RNA extracted from serum of cancer patients, 44 LC, 45 BC, 27 CRC, and 40 PCa, and on 45 HDs. The cancer detection performance of the miRNA expression levels was evaluated by studying the area under the curve (AUC) of receiver operating characteristic (ROC) curves at univariate and multivariate levels. miR-21 was significantly overexpressed in all cancer types compared with HDs, with accuracy of 67.5% (p = 0.001) for all 4 tumor types and of 80.8% (p < 0.0001) when PCa cases were removed from the analysis. For each tumor type, a panel of miRNAs was defined that provided cancer-detection accuracies of 91%, 94%, 89%, and 77%, respectively. In conclusion, we have described a series of circulating miRNAs that define different tumor types with a very high diagnostic performance. These panels of miRNAs would constitute the basis of different approaches of cancer-detection systems for which clinical utility should be validated in prospective cohorts.

MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

“…Recently, Pastor-Navarro and García-Flores et al proposed using a panel of four miRNAs for serum PCa diagnosis, including miR-375-3p, miR-182-5p, and miR-21-5p [ 35 ]. They showed that these miRNAs have great potential for PCa diagnostics, regarding their AUC values [ 35 ]. Again, our findings encourage these conclusions, showing that miR-182-5p and miR-375-3p deserve and require focus in further research.…”

Investigating the Role of Circulating miRNAs as Biomarkers in Colorectal Cancer: An Epidemiological Systematic Review

“…Overall, many dysregulated miRNAs were found, but most of them were not consistently analysed in more than one study. MiR-21 was the most-reported microRNA over the included studies, with 16 studies ([ 14 , 17 , 19 , 21 , 29 , 36 , 37 , 38 , 41 , 44 , 45 , 50 , 51 , 55 ], followed by miR-31([ 14 , 31 , 50 ]), miR-15b ([ 14 , 36 , 53 ]), and miR-20a ([ 15 , 39 , 48 ]), which were used in three studies each. However, in these studies, the expression of miR-20a did not have coherent values.…”

A novel β-cyclodextrin-assisted enhancement strategy for portable and sensitive detection of miR-21 in human serum

“…The pretreatment method was slightly modified on the basis of the existing literature. 37–39 2.5 mL of serum was taken and incubated at 95 °C for 15 min, cooled on ice for 3 min, centrifuged at 17 000× g at 4 °C for 15 min, and MNPs-cDNA was added to the supernatant for capture and enrichment of miR-21 (the final volume was 200 μL, the concentration of miR-21 was concentrated by 12.5 times). The established method was used to detect and calculate the concentration of miR-21 in the original serum.…”