A technique of heterotopic, infrarenal heart transplantation with double anastomosis in mice

Heterotopic cardiac xenotransplantation in rodents: Report of a refined technique in a hamster‐to‐rat model

“…On the other hand, there is a lot to learn from rodent models. 19,20 Indeed, as far as xenotransplantation is concerned, the hamster-to-rat combination is widely used and considered a concordant-difficult combination resembling the current immunological situation observed when transplanting genetically engineered porcine organs into primates. 21,22 The hamster-to-rat heterotopic cardiac xenotransplantation model is also a key tool for immunological and tolerance studies.…”

Potential of Heterotopic Cardiac Transplantation in Mice as a Model for Elucidating Mechanisms of Graft Rejection

“…Other research groups refer to a simplified and technically easier model of vascularized transplantation in which the graft is anastomosed to the cervical vessels [Chen, 1991;Tomita et al, 1997]. However, according to Doenst et al, first, the positioning of the transplanted heart in an infrarenal position is self-guided and less likely to allow torsion, and second, the carotid artery is smaller in diameter than the ascending aorta of the donor, what makes the aorto-aortic anastomosis easier to perform [Doenst et al, 2001]. Finally the decision of which operative procedure is performed depends on the personal preference.…”

Differential changes in respiratory capacity and ischemia tolerance of isolated mitochondria from atrophied and hypertrophied hearts

“…Ventricular unloading was achieved by infrarenal heterotopic heart transplantation in male Wistar rats [22,23]. Hearts were transplanted by anastomosing end to side the ascending aorta of the donor to the abdominal aorta of the recipient and the donor pulmonary artery to the recipient inferior vena cava.…”