A technique for determining the shear sensitivity of mammalian cells in suspension culture

Smith, Craig G.; Greenfield, P. F.; Randerson, David H.

doi:10.1007/bf00156285

Cited by 51 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be seen that the threshold value of the hydrodynamic stress for CHO cells is between 0.59 and 1.19 Pa while for HEK cells it is between 1.19 and 1.67 Pa, consistent with the FACS measurements and values reported in the literature (Abu-Reesh and Kargi, 1989;Petersen et al, 1988;Smith et al, 1987).…”

Section: Resultssupporting

confidence: 89%

“…Necrotic death in rheometers is reported at shear stresses in the range of 0.5-5 Pa (under laminar and turbulent conditions) (Abu-Reesh and Kargi, 1989;Petersen et al, 1988;Smith et al, 1987). Another device used to study necrotic cell death are capillaries, for which critical wall shear stress values between 100 and 240 Pa (at Re % 4,000-5,100) with and without dependency of the cell lysis rate on the capillary length (i.e., the residence time) were found (Ma et al, 2002;McQueen et al, 1987;Zhang et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of mammalian cell death by simple shear and extensional flows

Tanzeglock

Šoóš

Stephanopoulos

et al. 2009

Biotech & Bioengineering

View full text Add to dashboard Cite

In this work we investigated whether the type of shear flow, to which cells are exposed, influences the initiation of cell death. It is shown that mammalian cells, indeed, distinguish between discrete types of flow and respond differently. Two flow devices were employed to impose accurate hydrodynamic flow fields: uniform steady simple shear flow and oscillating extensional flow. To distinguish between necrotic and apoptotic cell death, fluorescence activated cell sorting and the release of DNA in the culture supernatant was used. Results show that Chinese Hamster Ovaries and Human Embryonic Kidney cells will enter the apoptotic pathway when subjected to low levels of hydrodynamic stress (around 2.0 Pa) in oscillating, extensional flow. In contrast, necrotic death prevails when the cells are exposed to hydrodynamic stresses around 1.0 Pa in simple shear flow or around 500 Pa in extensional flow. These threshold values at which cells enter the respective death pathway should be avoided when culturing cells for recombinant protein production to enhance culture longevity and productivity.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Induction of mammalian cell death by simple shear and extensional flows

Tanzeglock

Šoóš

Stephanopoulos

et al. 2009

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Smith et a1. 16 investigated the shear sensitivity of mouse hybridoma cells in suspension culture at constant shear rates in a Couette viscometer over a period of 15 h (approximately the cell doubling time). Live cell counts, cell viability, and the release of the cytoplasmic enzyme lactate dehydrogenase (LDH) into the culture medium were measured to characterize cellular damage.…”

Section: Hybridoma Cellsmentioning

confidence: 99%

A Review of the Effects of Shear and Interfacial Phenomena on Cell Viability

Hua

Erickson

Yiin

et al. 1993

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

The shear sensitivity of animal and plant cells is a problem often encountered in large-scale cell culture. Such sensitivity varies with different cell lines and the severity of cellular damage may depend on both the magnitude and the duration of the shear stress. In a bioreactor, the shear susceptibility of cells depends on their response to hydrodynamic forces arising from fluid motions of particular scale. Cell damage may be induced by forces in the bulk liquid phase, but fluid motions associated with the gas-liquid interface are especially energetic. The detrimental effects of hydrodynamic forces are abated by the addition of some polymers, such as Pluronic F-68, methylcellulose, or serum; the exact mechanisms of protection are the subject of current research.

show abstract

“…Current indirect methods for studying the effects of shear and other forces on mammalian cells in suspension culture (Stathopoulos and Hellums 1985;Smith et al 1987;Petersen et al 1988;Cherry and Kwon 1990) are unsatisfactory because the results of the experiments are difficult to interpret. A need therefore remains for a technique to directly measure cell mechanical properties (including bursting strength).…”

Section: Introductionmentioning

confidence: 99%

A novel micromanipulation technique for measuring the bursting strength of single mammalian cells

Zhang

Ferenczi²,

Lush³

et al. 1991

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Information about the bursting strength of animal cells is essential if the mechanisms of cell damage in bioreactors are to be understood, and if cell mechanical properties are ever to be related to cell structure and physiology. We have developed a novel cell compression technique that makes it possible to directly measure the bursting strength of single mammalian cells, and to infer information about cell mechanical properties.

show abstract

A technique for determining the shear sensitivity of mammalian cells in suspension culture

Cited by 51 publications

References 4 publications

Induction of mammalian cell death by simple shear and extensional flows

Induction of mammalian cell death by simple shear and extensional flows

A Review of the Effects of Shear and Interfacial Phenomena on Cell Viability

A novel micromanipulation technique for measuring the bursting strength of single mammalian cells

Contact Info

Product

Resources

About