A Technique for Clearing and Staining Gymnosperm Leaves

Shobe, W. Randall; Lersten, Nels R.

doi:10.1086/336391

Cited by 112 publications

(59 citation statements)

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“…Para a observação do padrão de nervação, foram diafanizadas folhas adotando-se a técnica de Shobe & Lersten (1967) com modifi cações, como a substituição do cloral hidratado por hipoclorito de sódio. Após este processo e posterior coloração com safranina, as folhas foram montadas em lâminas de vidro temporárias e fotografadas com auxílio de negatoscópio e de câmera digital SONY Cyber Shot modelo DSC-S650 com resolução de 7.2 MP.…”

Section: Methodsunclassified

Aplicação de caracteres morfoanatômicos foliares na taxonomia de Campomanesia Ruiz & Pavón (Myrtaceae)

et al. 2011

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ABSTRACT(Application of morpho-anatomical leaf characters in Campomanesia Ruiz & Pavón (Myrtaceae) taxonomy). To evaluate the utility of morpho-anatomical leaf characters in the identifi cation of botanic material of Campomanesia Ruiz & Pavón, especially when sterile, leaf venation pattern and shape of the vascular arch in the petiole of 15 taxa in Bahia state were analysed. Th e camptodromous-brochydodromous secondary venation pattern was uniform among the taxa, as well as the ultimate marginal venation in arches and orthogonal reticulate tertiary venation. Diff erences were observed in the presence of intersecondary veins and areolation in the leaf blade, and shape of the vascular arch and nature of the vascular bundle sheaths in the petiole, characters that could be used for taxonomic purposes.

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Section: Methodsunclassified

Aplicação de caracteres morfoanatômicos foliares na taxonomia de Campomanesia Ruiz & Pavón (Myrtaceae)

et al. 2011

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“…Whole leaves and petals of H. perforatum were cleared and stained by the method of Shobe & Lersten (1967) or Lersten (1986). The thick leaves of H. balearicum could not be cleared adequately.…”

Section: Methodsmentioning

confidence: 99%

Internal secretory structures in Hypericum (Clusiaceae): H. perforatum L. and H. balearicum L.

Curtis

Lersten

1990

New Phytologist

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SUMMARYHypericum species have translucent oil cavities; many species also have superficially similar black internal structures. Contradictory descriptions of both structures emerged from 19th century studies, which we review here. Our study of Hypericum perforatum L. leaves and petals shows typical schizogenous translucent oil cavities that develop with a uniseriate epithelium. Black structures, m contrast, are nodules, each composed ot several large cells lacking a central intercellular space and surrounded by a biseriate sheath of flattened cells. Nodule cells accumulate black, granular, but non-resinous contents. The 'streaks' that traverse petals are elongate tubular cavities or nodules, except for some chimerical tubes composed partly of each. Hypericum balearicum L. leaves have bulging, pustular cavities. A large cellular nodule forms first, by non-synchronous cell divisions within the leaf mesophyll, and is surrounded by a sheath of two to three flattened cell layers. The central cells then separate unevenly to form an irregular cavity into which isolated cells and clusters of cells intrude, some of which continue to divide as other cells degenerate. Little or no oil accumulates in the cavity, and the peripheral lining of cells is very irregular to flattened and empty. Both species studied exhibit secretory structures with previously undescribed anatomical features.

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“…Para visualização e classificação dos estômatos nas faces adaxial e abaxial, 5 folhas inteiras de cada espécie foram diafanizadas por aproximadamente 1 hora em solução de hidróxido de sódio a 10% e posteriormente em solução de hipoclorito de sódio a 25% por tempo determinado de acordo com a necessidade do material. As folhas foram coradas com safranina 1% (Shobe & Lersten, 1967).…”

Section: Methodsunclassified

“…O material seccionado foi selecionado e clarificado em solução de hipoclorito de sódio a 10%, corado com azul de alcian 1% (Luque et al, 1996) e safranina 1% (Shobe & Lersten, 1967), e posteriormente foram montadas lâminas semipermanentes.…”

Section: Methodsunclassified

Anatomia e histoquímica da lâmina foliar de espécies conhecidas por quebra-pedra (Euphorbia prostrata Aiton, Euphorbia hyssopifolia L., Phyllanthus amarus Schumach. & Thonn e Phyllanthus tenellus Roxb.)

Conceição

Aoyama

2016

Rev. bras. plantas med.

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RESUMO:O presente trabalho teve por objetivo realizar estudo anatômico comparativo entre duas espécies de Euphorbia L. e duas de Phyllanthus L. conhecidas popularmente por quebrapedra, a fim de contribuir com a identificação das espécies, visando o controle de qualidade e testes histoquímicos para conhecimento e localização de substâncias, com intuito de fornecer subsídios para futuros estudos. As quatro espécies estudadas foram coletadas na cidade de São Mateus-ES. Foram realizadas análises anatômicas e histoquímicas da lâmina foliar. Em vista frontal, E. prostrata apresenta paredes celulares poligonais, enquanto em E. hyssopifolia as paredes são sinuosas. As espécies de Euphorbia se diferenciam pela presença de canais laticíferos e estrutura Kranz. Somente nas espécies de Phyllanthus foram observados cristais de oxalato de cálcio. P. tenellus difere de P. amarus pela presença de cristais romboédricos e P. amarus por exibir cristais prismáticos no mesofilo. O estudo anatômico foliar foi de grande relevância para a diferenciação das espécies conhecidas por quebra-pedra, revelando ser um parâmetro eficaz e que pode auxiliar no controle de qualidade de plantas medicinais, bem como os testes histoquímicos podem fornecer informações para futuros estudos com quebra-pedra.Palavras-chave: Euphorbiaceae, Phyllanthaceae, Planta medicinal.ABSTRACT: Leaf anatomy and histochemistry of species known as stonebreaker (Euphorbia prostrata Aiton, Euphorbia hyssopifolia L., Phyllanthus amarus Schumach. & Thonn, and Phyllanthus tenellus Roxb.). This study aimed to compare the anatomical and histochemical perspective between two species of Euphorbia L. and two of Phyllanthus L., to contribute to their identification, aiming the quality control of medicinal plans. The four studied species were collected in the city of São Mateus, ES, Brazil. We performed anatomical and histochemical analyses of leaves, and all parameters were effective for species differentiation. In the anatomical analyses, we observed crystals only in the Phyllanthus species. Euphorbia species differed by the presence of laticifer channels and Kranz structure. In frontal and crosssectional view, P. tenellus differs from P. amarus by the presence of rhombohedral crystals. In front view, E. prostate presents polygonal cell walls, while in E. hyssopifolia the walls are winding. In the histochemical tests conducted on the leaf blade, P. amarus stands out by presenting negative reaction to ferric chloride. The anatomical and histochemical studies of leaves are really important to the knowledge and differentiation of species known as stonebreaker. However, phytochemical studies are needed for more detailed knowledge of substances from the secondary metabolism of the plant.

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A Technique for Clearing and Staining Gymnosperm Leaves

Cited by 112 publications

References 5 publications

Aplicação de caracteres morfoanatômicos foliares na taxonomia de Campomanesia Ruiz & Pavón (Myrtaceae)

Aplicação de caracteres morfoanatômicos foliares na taxonomia de Campomanesia Ruiz & Pavón (Myrtaceae)

Internal secretory structures in Hypericum (Clusiaceae): H. perforatum L. and H. balearicum L.

Anatomia e histoquímica da lâmina foliar de espécies conhecidas por quebra-pedra (Euphorbia prostrata Aiton, Euphorbia hyssopifolia L., Phyllanthus amarus Schumach. & Thonn e Phyllanthus tenellus Roxb.)

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