A Technique for Artificial Inoculation of Cauliflower Seedlings with <i>Sclerotinia sclerotiorum</i> (Lib.) de Bary

Kapoor, K. S.; Gill, H. S.; Sharma, S.R.

doi:10.1111/j.1439-0434.1985.tb04827.x

Cited by 7 publications

(4 citation statements)

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“…The scraped bacterial culture was mixed in 100 ml sterilized distilled water and mixed thoroughly by vortex and final concentration of 10 8 –10 9 cfu/ml was made. The plants were first inoculated on 30th day after planting by using leaf cut and dip technique (Kapoor et al, 1985 ) by clipping the secondary veins at the margins with small scissor dipped in the bacterial suspension. The inoculation was carried out at 10 points per leaf on youngest leaves in three replications.…”

Section: Methodsmentioning

confidence: 99%

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

et al. 2017

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Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is a very important disease of cauliflower (Brassica oleracea botrytis group) resulting into 10–50% yield losses every year. Since there is a dearth of availability of resistance to black rot disease in B. oleracea (C genome), therefore exploration of A and B genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. To utilize these sources, interspecific hybrid and backcross progeny (B1) were generated between cauliflower “Pusa Sharad” and Ethiopian mustard “NPC-9” employing in vitro embryo rescue technique. Direct ovule culture method was better than siliqua culture under different temperature regime periods. Hybridity testing of F1 inter-specific plants was carried out using co-dominant SSR marker and Brassica B and C genome-specific (DB and DC) primers. Meiosis in the di-genomic (BCC) interspecific hybrid of B. oleracea botrytis group (2n = 18, CC) × B. carinata (2n = 4x = 34, BBCC) was higly disorganized and cytological analysis of pollen mother cells revealed chromosomes 2n = 26 at metaphase-I. Fertile giant pollen grain formation was observed frequently in interspecific F1 hybrid and BC1 plants. The F1 inter-specific plants were found to be resistant to Xcc race 1. Segregation distortion was observed in BC1 generation for black rot resistance and different morphological traits. The At1g70610 marker analysis confirmed successful introgression of black rot resistance in interspecific BC1 population. This effort will go a long way in pyramiding gene(s) for resistance against black rot in Cole crops, especially cauliflower and cabbage for developing durable resistance, thus minimize dependency on bactericides.

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Section: Methodsmentioning

confidence: 99%

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

et al. 2017

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“…Three inner whorl young leaves during seedling stage (15 DAT) and adult plant stage (45 DAT) were inoculated using leaf cut and dip technique by dipping the scissor in bacterial solution and cutting the tip of the leaves (Kapoor et al. ). The inoculated plants were scored three times for each inoculation at 20‐day interval up to 60 days after inoculation (DAI) according to 0–4 scale (Tewari et al.…”

Section: Methodsmentioning

confidence: 99%

Molecular mapping of black rot resistance locusXca1boon chromosome 3 inIndian cauliflower (Brassica oleraceavar.botrytisL.)

et al. 2014

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Black rot is the most devastating disease of cauliflower worldwide causing severe damage to crop. The identification of markers linked to loci that control resistance can facilitate selection of plants for breeding programmes. In the present investigation, F 2 population derived from a cross between 'Pusa Himjyoti', a susceptible genotype, and 'BR-161', a resistant genotype, was phenotyped by artificial inoculation using Xcc race 1. Segregation analysis of F 2 progeny indicated that a single dominant locus governed resistance to Xcc race 1 in 'BR-161'. Bulk segregant analysis in resistant and susceptible bulks of F 2 progeny revealed seven differentiating polymorphic markers (three RAPD, two ISSR and two SSR) of 102 markers screened. Subsequently, these markers were used to genotype the entire F 2 population, and a genetic linkage map covering 74.7 cM distance was developed. The major locus Xca1bo was mapped in 1.6-cM interval flanked by the markers RAPD 04 833 and ISSR 11 635 . The Xca1bo locus was located on chromosome 3. The linked markers will be useful for marker-assisted resistance breeding in cauliflower.

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“…The first inoculation was done at 15 days after transplanting and the second at 45 days after transplanting. Three inner whorl young leaves were selected in each plant and inoculated by following leaf cut and dip technique as described by Kapoor et al ( 1985 ). The scoring of inoculated plants was done in a scale of 0–4 for three times in each inoculation at 20 days interval as per Tewari et al ( 1987 ).…”

Section: Methodsmentioning

confidence: 99%

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

et al. 2021

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Cauliflower is an important extensively grown cool season vegetable in India. Black rot and downy mildew are major devastating diseases reducing yield and quality of the crop. To tackle these through host plant resistance, a marker-assisted backcross breeding method was followed to pyramid a black rot-resistant gene (Xca1bo) and a downy mildew-resistant gene (Ppa3) from donors BR-161 and BR-2, respectively, into the background of Pusa Meghna cauliflower cultivar. Marker-assisted backcross breeding was followed up to BC2 generation using SCAR marker ScOPO-04833 and SSR marker BoGMS0624 for black rot and downy mildew resistance genes in foreground selection, respectively. In background selection, at each stage of backcrossing, 47 parental polymorphic SSR markers were used. The graphical genotyping of the five two-gene (Xca1boXca1boPpa3Ppa3) homozygous BC2F2 plants showed an average recovery of 85.44% of the Pusa Meghna genome with highest genome recovery of 91.7%. The genome contribution of donor parents (BR-161 and BR-2) was 8.26 with 6.34% of residual heterozygousity. The backcross derived pyramided lines BC2F2:3-7-16 and BC2F2:3-7-33 showed high resistance to both the diseases and exhibited higher yield and vitamin C content as compared with recipient parent Pusa Meghna. It is, therefore, evident from this study that resistant genes can be introgressed successfully into a Pusa Meghna cultivar without any yield penalty, benefitting farmers with reduced input cost and consumers with chemical residue free produce. Besides, the pyramided lines carrying dominant resistant genes can be exploited in a hybridization programme to develop hybrid(s) in cauliflower.

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A Technique for Artificial Inoculation of Cauliflower Seedlings with Sclerotinia sclerotiorum (Lib.) de Bary

Cited by 7 publications

References 1 publication

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

Molecular mapping of black rot resistance locusXca1boon chromosome 3 inIndian cauliflower (Brassica oleraceavar.botrytisL.)

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

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