A systemic and molecular study of subcellular localization of SARS-CoV-2 proteins

Zhang, Jing; Cruz-Cosme, Ruth; Zhuang, Meng; Liu, Dongxiao; Liu, Yuan; Teng, Shaolei; Wang, Pei‐Hui; Tang, Qiyi

doi:10.1038/s41392-020-00372-8

Cited by 130 publications

(119 citation statements)

References 5 publications

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“…CLCC1 is characterized as an endoplasmic reticulum protein, which was confirmed by our immunofluorescence results in cells without ORF3a overexpression. ORF3a is reported to be an endosome and lysosome-associated protein 12 , which was also validated by our immunofluorescence results. When ORF3a was overexpressed, the localization of CLCC1 changed to strong co-localization with ORF3a in the endosome and lysosome.…”

Section: Sars-cov-2 Interacts With Host Proteins Which May Facilitatsupporting

confidence: 85%

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

Chen

Wang

Feng

et al. 2021

Preprint

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Host-virus protein-protein interaction is the key component of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lifecycle. We conducted a comprehensive interactome study between the virus and host cells using tandem affinity purification and proximity labeling strategies and identified 437 human proteins as the high-confidence interacting proteins. Functional characterization and further validation of these interactions elucidated how distinct SARS-CoV-2 viral proteins participate in its lifecycle, and discovered potential drug targets to the treatment of COVID-19. The interactomes of two key SARS-CoV-2 encoded viral proteins, NSP1 and N protein, were compared with the interactomes of their counterparts in other human coronaviruses. These comparisons not only revealed common host pathways these viruses manipulate for their survival, but also showed divergent protein-protein interactions that may explain differences in disease pathology. This comprehensive interactome of coronavirus disease-2019 provides valuable resources for understanding and treating this disease.

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Section: Sars-cov-2 Interacts With Host Proteins Which May Facilitatsupporting

confidence: 85%

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

Chen

Wang

Feng

et al. 2021

Preprint

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“…After transfection, cells were stimulated with 1000 IU/mL human IFN-α-2b for the indicated time as described previously [30] . All the SARS and SARS-CoV-2 protein constructs were constructed and determined to be successfully expressed as we described previously [31] .…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Spike protein enhances ACE2 expression via facilitating Interferon effects in bronchial epithelium

Zhou

Wang

et al. 2021

Immunology Letters

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Objective In this study, we focused on the interaction between SARS-CoV-2 and host Type I Interferon (IFN) response, so as to identify whether IFN effects could be influenced by the products of SARS-CoV-2. Methods All the structural and non-structural proteins of SARS-CoV-2 were transfected and overexpressed in the bronchial epithelial cell line BEAS-2B respectively, and typical antiviral IFN-stimulated gene (ISG) ISG15 expression was detected by qRT-PCR. RNA-seq based transcriptome analysis was performed between control and Spike (S) protein-overexpressed BEAS-2B cells. The expression of ACE2 and IFN effector JAK-STAT signaling activation were detected in control and S protein-overexpressed BEAS-2B cells by qRT-PCR or/and Western blot respectively. The interaction between S protein with STAT1 and STAT2, and the association between JAK1 with downstream STAT1 and STAT2 were measured in BEAS-2B cells by co-immunoprecipitation (co-IP). Results S protein could activate IFN effects and downstream ISGs expression. By transcriptome analysis, overexpression of S protein induced a set of genes expression, including series of ISGs and the SARS-CoV-2 receptor ACE2. Mechanistically, S protein enhanced the association between the upstream JAK1 and downstream STAT1 and STAT2, so as to promote STAT1 and STAT2 phosphorylation and ACE2 expression. Conclusion SARS-CoV-2 S protein enhances ACE2 expression via facilitating IFN effects, which may help its infection.

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“…The SARS-CoV-2 M-FLAG and N-FLAG vectors were kindly provided by Dr. Pei-Hui Wang 43 . HEK293T cells were co-transfected with 10 μg Ha-CoV-2(Luc), 2.5 μg of the SARS-CoV-2 S expression vector, and 2.5 μg each of the M-FLAG, N-FLAG, and E-FLAG vectors.…”

Section: Methodsmentioning

confidence: 99%

Development of a novel hybrid alphavirus-SARS-CoV-2 particle for rapid in vitro screening and quantification of neutralization antibodies, viral variants, and antiviral drugs

Hetrick

Chilin

et al. 2020

Preprint

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SUMMARYTimely development of vaccines and antiviral drugs are critical to control the coronavirus disease 2019 (COVID-19) global pandemic 1–6. Current methods for validation of vaccine efficacy involve the use of pseudoviruses, such as the SARS-CoV-2 spike protein (S) pseudotyped lentivirus or vesicular stomatitis virus (VSV), to quantify neutralizing antibodies for blocking viral infection 7–14. The process of pseudovirus infection and quantification is time consuming and can take days to complete. In addition, pseudoviruses contain structural proteins not native to SARS-CoV-2, which may alter particle properties in receptor binding and responses to antibody neutralization 15. Here we describe the development of a new hybrid alphavirus-SARS-CoV-2 particle (Ha-CoV-2) for rapid screening and quantification of neutralization antibodies and antiviral drugs. Ha-CoV-2 is a non-replicating SARS-CoV-2 virus-like particle, composed of only SARS-CoV-2 structural proteins (S, M, N, and E) and a RNA genome derived from a fast expressing alphavirus vector 16. We demonstrate that Ha-CoV-2 can rapidly and robustly express reporter genes in target cells within 3-5 hours following viral entry. We further validate the Ha-CoV-2 system for rapid quantification of neutralization antibodies and antiviral drugs. In addition, we assembled a Ha-CoV-2 particle bearing the D614G mutant spike protein, and found that the mutation led to an approximately 200% increase in virion infectivity. These results demonstrate that Ha-CoV-2 can also be applied for rapid monitoring and quantification of viral mutations for effects on neutralizing antibodies induced by vaccines.

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A systemic and molecular study of subcellular localization of SARS-CoV-2 proteins

Cited by 130 publications

References 5 publications

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

SARS-CoV-2 Spike protein enhances ACE2 expression via facilitating Interferon effects in bronchial epithelium

Development of a novel hybrid alphavirus-SARS-CoV-2 particle for rapid in vitro screening and quantification of neutralization antibodies, viral variants, and antiviral drugs

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