A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self‐assembled poly(ethylene oxide) triblock copolymer surfaces

Abstract

A well-controlled biocompatible nonfouling surface is significant for biomedical requirements, especially for the improvement of biocompatibility. We demonstrate the low or nonbiofouling surfaces by coating hydrophobic-hydrophilic triblock copolymers of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) on the CH(3)-terminated self-assembled monolayer (SAM). Two types of copolymers are used to modify the surface, one with different PEO/PPO ratios ( approximately 20/80, 40/60, and 80/20, w/w) but the same PPO molecular weight ( approximately 2 k), the other with different copolymer MWs ( approximately 9, 11, and 15 k) but the same PEO/PPO ratio (80/20, w/w). In situ surface plasmon resonance (SPR) sensor is used to evaluate polymer adsorption on the SAMs and subsequent protein adsorption on the copolymer-treated surface. The effects of PEO-PPO-PEO molecular weight, PPO-to-PEO ratio, and ionic strength on protein adsorption from single protein solutions of fibrinogen, BSA, and complex mixed proteins are systematically investigated. A Pluronic F108 treated surface is highly resistant to nonspecific protein adsorption under the optimized conditions (MW of 15 k and PEO/PPO ratio of 80/20). This work demonstrates that the PEO-PPO-PEO polymer is able to achieve ultra low fouling surface via surface modification by controlling surface packing density of polymers (molecular weight, hydrophobic/hydrophilic ratio, and hydrophilic group coverage).

Mechanisms of fibrinogen adsorption at the silica substrate determined by QCM-D measurements

“…Extensive studies have also been devoted to fibrinogen adsorption on various substrates performed mostly at pH 7.4 and ionic strength of 0.15 M, pertinent to physiological conditions [14][15][16][17][18][19][20]. A variety of experimental techniques was used such as ellipsometry [14][15][16], OWLS [16], TIRF and AFM [17,18], radiolabelling [19], SPR [20].…”

“…A variety of experimental techniques was used such as ellipsometry [14][15][16], OWLS [16], TIRF and AFM [17,18], radiolabelling [19], SPR [20].…”

Surface anti-biofouling control of PEGylated poly(vinylidene fluoride) membranes via vapor-induced phase separation processing

“…These hydrophilic properties can be brought by poly(ethylene oxide) (PEO)-or poly(ethylene glycol) (PEG)-based materials, the most commonly used biofouling-resistant materials, earlier used in numerous studies to design anti-biofouling surfaces [3][4][5].…”

Influence of solvent composition and non-solvent activity on the crystalline morphology of PVDF membranes prepared by VIPS process and on their arising mechanical properties

“…This meant that the solidliquid adhesion energy could be affected by simply modifying the VIPS testing conditions and the solvent composition. Usually, to improve membranes' hydrophilicity, an amphiphilic copolymer is used [32,33] or a change of process performed [30]. Here, it is clearly shown that the wetting of membranes is affected by physical effects owed to a change of testing parameters.…”