Mechanisms of fibrinogen adsorption at the silica substrate determined by QCM-D measurements

Kubiak, Katarzyna; Adamczyk, Zbǐgniew; Wasilewska, Monika

doi:10.1016/j.jcis.2015.07.009

Cited by 31 publications

(58 citation statements)

References 44 publications

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“…It has been suggested that fibrinogen may adsorb to negatively charged surfaces by means of electrostatic interactions between the net positively charged αC-domains and the surface [21][22][23][24]. However, in our present study, the αC-chain was found to have a net negative charge.…”

Section: Introductioncontrasting

confidence: 89%

“…It is probable that the experimental methods do not yield the surface charge exactly at the surface. Kubiak et al calculated a surface charge density by using Gouy-Chapman theory to convert ζ-potential to charge density [24]. Even though the charge of the slipping plane (where the ζ-potential is measured) should probably be considerably lower than the surface charge, they found the same charge magnitude as those measured by Bolt [34] and Samoshina et al [35].…”

Section: Adsorption Onto a Flat Surfacementioning

confidence: 91%

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Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles

et al. 2020

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When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Thus, modifications of the surface nanotopography of an implant may prevent biomaterial-associated infections. Fibrinogen is of particular importance since it contains adhesion epitopes that are recognized by both eukaryotic and prokaryotic cells, and can therefore influence the adhesion of bacteria. The aim of this study was to model adsorption of fibrinogen to smooth or nanostructured silica surfaces in an attempt to further understand how surface nanotopography may affect the orientation of the adsorbed fibrinogen molecule. We used a coarse-grained model, where the main body of fibrinogen (visible in the crystal structure) was modeled as rigid and the flexible α C-chains (not visible in the crystal structure) were modeled as completely disordered. We found that the elongated fibrinogen molecule preferably adsorbs in such a way that it protrudes further into solution on a nanostructured surface compared to a flat one. This implicates that the orientation on the flat surface increases its bio-availability.

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Section: Introductioncontrasting

confidence: 89%

Section: Adsorption Onto a Flat Surfacementioning

confidence: 91%

Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles

et al. 2020

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“…The benefits stemming from monitoring of dispersion changes include the option of discrimination between the types of amyloid adsorption [ 85 ] and characteristic fibril growth and conformations [ 25 ]. Formation of the fibrils often generates complex sensors response requiring application of appropriate sensor models and supplementary microscopic measurements, e.g., ex-situ AFM, fluorescence microscopy [ 25 ], or total internal reflection fluorescence (TIRF) [ 101 ]. One of the latest achievements is the elaboration of a combined QCM-TIRF technique allowing simultaneous measurement of the mass of a peptide adsorbed on the sensor’s surface and visualization of fibril growth using the TIRF microscope [ 24 ].…”

Section: Adsorption Of Peptide and Protein Molecules On The Qcm Sumentioning

confidence: 99%

Application of QCM in Peptide and Protein-Based Drug Product Development

2020

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AT-cut quartz crystals vibrating in the thickness-shear mode (TSM), especially quartz crystal resonators (QCRs), are well known as very efficient mass sensitive systems because of their sensitivity, accuracy, and biofunctionalization capacity. They are highly reliable in the measurement of the mass of deposited samples, in both gas and liquid matrices. Moreover, they offer real-time monitoring, as well as relatively low production and operation costs. These features make mass sensitive systems applicable in a wide range of different applications, including studies on protein and peptide primary packaging, formulation, and drug product manufacturing process development. This review summarizes the information on some particular implementations of quartz crystal microbalance (QCM) instruments in protein and peptide drug product development as well as their future prospects.

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“…Integration of QCM with MF devices has been utilized for different applications including studies of chemical reactions occurring in flow, variation in viscosity of ionic liquids, sensing of drugs by adsorption analysis, and adsorption of proteins . For instance, the kinetics of fibrinogen adsorption on silica substrates under different flow rates have been studied using QCM technique …”

Section: Characterization Of Polymer Adsorptionmentioning

confidence: 99%

Microfluidic Studies of Polymer Adsorption in Flow

Salari

Kumacheva

2016

Macro Chemistry & Physics

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Adsorption of polymer molecules under flow conditions governs a broad range of desired or undesired phenomena and has a broad range of applications. Microfluidic platforms integrated with analytical instrumentation offer the capability to study polymer adsorption in flow in a time‐ and labour‐efficient manner. Precise control of the microchannel dimensions and flow parameters enables accurate control of shear forces imposed on polymer solutions and on the molecules deposited on the surface, along with high sensitivity, enhanced reliability, and low reagent consumption. This review highlights recent advances in theoretical and experimental microfluidic studies of polymer immobilization under flow conditions in three area, namely, biosensing, biofouling, and studies of thrombosis and hemostasis.

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Mechanisms of fibrinogen adsorption at the silica substrate determined by QCM-D measurements

Cited by 31 publications

References 44 publications

Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles

Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles

Application of QCM in Peptide and Protein-Based Drug Product Development

Microfluidic Studies of Polymer Adsorption in Flow

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