A Synthetic System That Senses <i>Candida albicans</i> and Inhibits Virulence Factors

Tscherner, Michael; Giessen, Tobias W.; Markey, Laura; Kumamoto, Carol A.; Silver, Pamela A.

doi:10.1021/acssynbio.8b00457

Cited by 19 publications

(4 citation statements)

References 54 publications

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“…By using the large repertoire of synthetic biology tools, bacteria can be reprogrammed to specifically target a disease, resulting in a highly precise autonomous therapy. Such therapies have been developed to target metabolic diseases, − prevent cancer, − inhibit pathogens, , and address other conditions − (for recent reviews, see refs − ). An increasing number of such studies have been reported thus far.…”

Section: Strategies To Engineer the Microbiome For Therapeutic Applic...mentioning

confidence: 99%

Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies

et al. 2022

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The human microbiome is composed of a collection of dynamic microbial communities that inhabit various anatomical locations in the body. Accordingly, the coevolution of the microbiome with the host has resulted in these communities playing a profound role in promoting human health. Consequently, perturbations in the human microbiome can cause or exacerbate several diseases. In this Review, we present our current understanding of the relationship between human health and disease development, focusing on the microbiomes found across the digestive, respiratory, urinary, and reproductive systems as well as the skin. We further discuss various strategies by which the composition and function of the human microbiome can be modulated to exert a therapeutic effect on the host. Finally, we examine technologies such as multiomics approaches and cellular reprogramming of microbes that can enable significant advancements in microbiome research and engineering.

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Section: Strategies To Engineer the Microbiome For Therapeutic Applic...mentioning

confidence: 99%

Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies

et al. 2022

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“…These living biosensors are an alternative or complement to chemical or electrical based analytical methods that enable in situ , real-time, and low-cost sensing. Biosensors have been constructed in microbes to detect light, chemicals, biomolecules, pH, and temperature [ 83 , 92 – 95 ]. Cells are programmed to output fluorescent or colorimetric reporters, antibiotic resistance, toxins, or functional actuators, such as therapeutics.…”

Section: Applicationsmentioning

confidence: 99%

Engineered microbial consortia: strategies and applications

Duncker

Holmes

2021

Microb Cell Fact

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Many applications of microbial synthetic biology, such as metabolic engineering and biocomputing, are increasing in design complexity. Implementing complex tasks in single populations can be a challenge because large genetic circuits can be burdensome and difficult to optimize. To overcome these limitations, microbial consortia can be engineered to distribute complex tasks among multiple populations. Recent studies have made substantial progress in programming microbial consortia for both basic understanding and potential applications. Microbial consortia have been designed through diverse strategies, including programming mutualistic interactions, using programmed population control to prevent overgrowth of individual populations, and spatial segregation to reduce competition. Here, we highlight the role of microbial consortia in the advances of metabolic engineering, biofilm production for engineered living materials, biocomputing, and biosensing. Additionally, we discuss the challenges for future research in microbial consortia.

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“…Tscherner et al. utilized the hydroxyphenylacetic acid (HPA) transporter (HpaX), transcription factor (HpaA), and cognate promoter (P BC ) to detect HPA, which is produced by the fungus Candida albicans . Upon HPA detection, an enzyme was expressed to synthesize cis ‐2‐dodecenoic acid which inhibits the formation of C. albicans hypha.…”

Section: Biosensing For Smart Expression Of Therapeuticsmentioning

confidence: 99%

Biosensing in Smart Engineered Probiotics

Rottinghaus

Amrofell

Moon

2020

Biotechnology Journal

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Engineered microbes are exciting alternatives to current diagnostics and therapeutics. Researchers have developed a wide range of genetic tools and parts to engineer probiotic and commensal microbes. Among these tools and parts, biosensors allow the microbes to sense and record or to sense and respond to chemical and environmental signals in the body, enabling them to report on health conditions of the animal host and/or deliver therapeutics in a controlled manner. This review focuses on how biosensing is applied to engineer "smart" microbes for in vivo diagnostic, therapeutic, and biocontainment goals. Hurdles that need to be overcome when transitioning from high-throughput in vitro systems to low-throughput in vivo animal models, new technologies that can be implemented to alleviate this experimental gap, and areas where future advancements can be made to maximize the utility of biosensing for medical applications are also discussed. As technologies for engineering microbes continue to be developed, these engineered organisms will be used to address many medical challenges.

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A Synthetic System That Senses Candida albicans and Inhibits Virulence Factors

Cited by 19 publications

References 54 publications

Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies

Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies

Engineered microbial consortia: strategies and applications

Biosensing in Smart Engineered Probiotics

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