A Synthetic Peptide Based on a Natural Salivary Protein Reduces Demineralisation in Model Systems for Dental Caries and Erosion

Kosoric, Jelena; Williams, Ruth; Hector, Mark; Anderson, Paul

doi:10.1007/s10989-007-9085-0

Cited by 36 publications

(36 citation statements)

References 38 publications

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“…In an earlier report a statherin fragment, identical to the first 21 residues of statherin, StN21, was shown to reduce the rate of mineral loss of HA pellets (12.5 porosity) compared to the controls without the peptide. However, the reduction on the dissolution rate of HA did not show a linear dependence on the amount of StN21 adsorbed [11]. Therefore, the aim of this study was to investigate the concentration dependence of the StN21 and of a synthetic statherin on adsorption onto HA discs.…”

Section: Introductionmentioning

confidence: 97%

Adsorption behavior of statherin and a statherin peptide onto hydroxyapatite and silica surfaces by in situ ellipsometry

Santos

Kosoric

Hector

et al. 2008

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 97%

Adsorption behavior of statherin and a statherin peptide onto hydroxyapatite and silica surfaces by in situ ellipsometry

Santos

Kosoric

Hector

et al. 2008

Journal of Colloid and Interface Science

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“…Statherin's ability to bind calcium reduces demineralization in vitro [Kosoric et al, 2007] and by maintaining a high calcium concentration next to the tooth probably aids its remineralization following an acidic challenge. In addition, statherin acts as a boundary lubricant during tooth-to-tooth abrasion [Douglas et al, 1991;Hahn Berg et al, 2004;Harvey et al, 2011] whilst the salivary mucin MUC 5B [Makakova et al, 2011] lubricates in the wider mixed regime lubrication.…”

mentioning

confidence: 99%

Composition of Enamel Pellicle from Dental Erosion Patients

Carpenter¹,

Cotroneo²,

Moazzez³

et al. 2014

Caries Res

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Oral health is dependent upon a thin mobile film of saliva on soft and hard tissues. Salivary proteins adhere to teeth to form the acquired enamel pellicle which is believed to protect teeth from acid erosion. This study investigated whether patients suffering diet-induced dental erosion had altered enamel pellicles. Thirty patients suffering erosion were compared to healthy age-matched controls. Subjects wore a maxillary splint holding hydroxyapatite and human enamel blocks for 1 h. The acquired enamel pellicle was removed from the blocks and compared to the natural incisor pellicle. Basic Erosive Wear Examination scores confirmed that dental erosion was present in erosion patients and absent from healthy age-matched controls. Erosion patients had half the amount of proteins (BCA assay) within the acquired pellicle forming on splint blocks compared to normal controls (p < 0.05). In particular, statherin, a calcium-binding protein, was 35% less abundant (p < 0.05). Calcium concentration within the acquired pellicle was also reduced by 50% in erosion patients (p < 0.001). In contrast, the natural pellicle on the incisor had similar amounts of total protein in erosion patients and healthy controls. In summary, the formation of new acquired pellicles on surfaces was reduced in erosion patients, which may explain their greater susceptibility to acid erosion of teeth.

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“…4 d), it appears to work synergistically in combination with DCP, forming an effective DH desensitiser. These specific results were probably due to the anti-erosive characteristic of PHS which may capture ionised phosphate, and this activity may have a protection effect against demineralisation by binding any remaining HAp crystals [Kosoric et al, 2007]. However, due to its amphipathic character, in solution, PHS has the tendency to assemble into highly positively charged aggregates or micelles, with the fatty acid tails buried inside and the positively charged head groups exposed to the bulk of the solution.…”

Section: Discussionmentioning

confidence: 99%

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

et al. 2016

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The present investigation evaluated the ability of an experimental di-calcium phosphate (DCP) desensitising agent used alone or combined with phytosphingosine (PHS) to occlude dentine tubules and resist a citric acid (CA) or artificial saliva (AS) challenge. Three groups of human dentine specimens (DS) were treated with the following: (1) PHS alone, (2) DCP or (3) a combination of PHS and DCP. Dentine hydraulic conductance of DS was evaluated using a digital flow sensor at 6.9 kPa. The average fluid volume for each of the treated DS was used to calculate the total dentine permeability reduction (%P) prior to and following CA immersion for 1 min or AS immersion for 4 weeks. The treated DS were subjected to both scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy analysis. Statistically significant differences (%P) were identified between the groups by ANOVA and Fisher's multiple comparison test (p < 0.05), respectively. Interestingly, both PHS and DCP appeared to work synergistically. DS treated with DCP or PHS/DCP demonstrated a significant reduction (%P) prior to and following CA or AS challenge (p < 0.05). Both the SEM and FTIR analyses showed consistent brushite crystals occluding the dentine tubules. Conversely, the application of PHS alone failed to demonstrate any significant reduction of dentine permeability (p > 0.05) or show any evidence of occlusion of the dentine tubules. DCP can be used alone or combined with PHS to decrease the dentine permeability as well as to resist a CA and AS challenge. These results would, therefore, suggest that DCP may be a suitable treatment option for dentine hypersensitivity.

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A Synthetic Peptide Based on a Natural Salivary Protein Reduces Demineralisation in Model Systems for Dental Caries and Erosion

Cited by 36 publications

References 38 publications

Adsorption behavior of statherin and a statherin peptide onto hydroxyapatite and silica surfaces by in situ ellipsometry

Adsorption behavior of statherin and a statherin peptide onto hydroxyapatite and silica surfaces by in situ ellipsometry

Composition of Enamel Pellicle from Dental Erosion Patients

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

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