A Synthetic Medium for Bacterial Cellulose Production by<i>Acetobacter xylinum</i>subsp.<i>sucrofermentans</i>

Matsuoka, Masanobu; Tsuchida, Takayasu; Matsushita, Kazunobu; Adachi, Osao; Yoshinaga, Fumihiro

doi:10.1271/bbb.60.575

Cited by 190 publications

(95 citation statements)

References 3 publications

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“…dense layer) were produced and purified as described elsewhere [8]. Briefly, a suspension of Gluconacetobacter xylinus (ATCC ® 700178, LGC Standards, Sweden) was inoculated in 250 ml conical flasks containing sterile culture medium (described by Matsuoka et al [43]) and cultured at 30°C for 18 days, until large BNC cylinders (Ø 48 mm × 20 mm) were biosynthesized. The BNC cylinders were purified in a built-in-house perfusion system and compressed to 1 mm in height to increase the cellulose content.…”

Section: Fabrication and Purification Of Bilayer Bnc Scaffoldsmentioning

confidence: 99%

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

et al. 2015

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Section: Fabrication and Purification Of Bilayer Bnc Scaffoldsmentioning

confidence: 99%

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

et al. 2015

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“…A 3 mL bacteria suspension and 300 mL of sterile culture media (described by Matsuoka et al (Matsuoka et al, 1996)) was added to each flask. The conical flasks were placed in an incubator at 30°C.…”

Section: Bnc Synthesismentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

193

108

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3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Secondly, the capacity of BNC to match these requirements is assessed. Finally, a biofabrication process to produce patient-specific BNC auricular implants is demonstrated.BNC samples (n = 78) with varying cellulose content (2.5 -15%) were compared using stressrelaxation indentation with human ear cartilage (n = 17, from 4 males, aged 49 -93 years old).Additionally, an auricle from a volunteer was scanned using a 3T MRI with a spoiled gradientecho sequence. A negative ear mold was produced from the MRI data in order to investigate if an ear-shaped BNC prototype could be produced from this mold.The results show that the instantaneous modulus E in , equilibrium modulus E eq , and maximum stress σ max of the BNC samples are correlated to effective cellulose content. Despite significantly different relaxation kinetics, the E in , E eq and σ max of BNC at 14% effective cellulose content reached values equivalent to ear cartilage (for E eq , BNC: 2.4 ± 0.4 MPa and ear cartilage: 3.3 ± 1.3 MPa). Additionally, this work shows that BNC can be fabricated into patient-specific auricular shapes. In conclusion, BNC has the capability to reach mechanical properties of relevance for ear cartilage replacement, and can be produced in patient-specific ear shapes.

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“…After the bioreactors had been cooled to room temperature they were inoculated with A. xylinum subspecies sucrofermentas BPR2001, trade number 700178™ (LGC Promochem AB, Borås, Sweden). A volume of 2.5 ml of 3.7 Â 10 6 cfu ml À1 bacteria in culture medium (described elsewhere by Matsuoka et al [19]) was added to each bioreactor. The bacteria were forced down into the paraffin wax bed by applying a small vacuum and pipetting the cell suspension up and down.…”

Section: Fermentation Of Microporous Bacterial Cellulosementioning

confidence: 99%

Microporous bacterial cellulose as a potential scaffold for bone regeneration

et al. 2010

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a b s t r a c tNanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 lm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

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A Synthetic Medium for Bacterial Cellulose Production byAcetobacter xylinumsubsp.sucrofermentans

Cited by 190 publications

References 3 publications

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Microporous bacterial cellulose as a potential scaffold for bone regeneration

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