A Switchable Linker‐Based Immunoassay for Ultrasensitive Visible Detection of Salmonella in Tomatoes

Abstract

The novel switchable linker-based immunoassay is a rapid, specific, and sensitive method that has potential applications for routine diagnostics of Salmonella in tomato products. These advantages make it a practical approach for general use in the processing industry to detect Salmonella rapidly and to implement appropriate regulatory procedures. Furthermore, it could be applied to other fresh products including cantaloupe, strawberry, and cucumbers.

Development of a portable lab-on-a-valve device for making primary diagnoses based on gold-nanoparticle aggregation induced by a switchable linker

“…Typhimurium, which conrmed the selectivity of SL-based assay in the previous study, was used as a representative strain. 35 As shown in Fig. 6, the REVC shi occurred at a linker concentration of 15 mg mL À1 , as compared to the control in the tested Salmonella Typhimurium samples (10 2 , 10 4 , and 10 6 CFU/ 400 mL).…”

“…In addition, in previous studies, we reported that the SL-based assay could work in serum as well as in various food matrices such as tomato and milk. [34][35][36][37] Therefore, we dealed with the minimum environmental factors (salt and pH) in this study.…”

“…This kit is based on the use of colorimetric detection by the precipitation of gold nano-aggregates (Au NAs) and a novel switchable linker to amplify the visible signal by the aggregation of gold nanoparticles. [34][35][36][37] In addition, for quick on-site detection, we introduced ltration into the device as a strategy for improving the visual detection signal.…”

LSPR‐based colorimetric biosensing for food quality and safety

“…Emerging detection methods are independent of gene amplification, and the common strategy is based on the surface modification of AuNPs with Abs, proteins, and aptamers. The peak absorbance exhibits a red shift with an increase in size and/or aggregation of AuNPs, giving stable AuNPs' solution red color, while the aggregated state of AuNPs produces purple color (Hahn et al, 2017;Sanromán-Iglesias et al, 2015;You et al, 2020).…”

Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked immunosorbent assay, bacteriophage amplification, and gold nanoparticle aggregation) methods in food samples: A review

“…Further, this method was used for the detection of Salmonella spp. in tomatoes (Hahn et al, 2017) with agitation, which was a step suggested for more rapid detection. The detection limit of Salmonella in tomato was 10 CFU/g.…”