A survey of the available colorimetric indicators for Ca2+ and Mg2+ ions in biological experiments

Durham, Anthony C.H.; Walton, Jill M.

doi:10.1016/0143-4160(83)90048-9

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…Arsenazo III and the similar' dye chlorophosphonazo III combine strongly with Ca2", the atom fitting between the phenol hydroxy and the arsono groups. Dyes such as Eriochrome Black T and Eriochrome Blue SE, which are selective for Mg2", have azo groups ortho to the phenolic groups (see Durham & Walton, 1983), in which the Mg2" could bind to the nitrogen as well as to the oxygen functions. They cannot give a complex comparable with that shown above for Ca2+-arsenazo III, since these reagents have no suitably placed oxyanions such as arsono groups.…”

Section: Ca2lmentioning

confidence: 99%

The interaction of cations with the dye arsenazo III

Rowatt

Williams

1989

Biochemical Journal

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1. The dye arsenazo III combines with a selection of cations to give an altered absorption spectrum. 2. Large metal cations such as Ca2+, La3+ and quadrivalent cations give a 1:1 complex with two new absorption peaks at about 610 nm and 655 nm and a KD of about 10(-6) M. 3. Aliphatic polyamines and complex cobalt ions give a 1:1 complex, with one absorption peak at about 610 nm and a KD from 10(-6) to 10(-3) M. 4. Small metal cations finally form a 2:1 complex and also have one absorption peak at about 610 nm, but with a KD of 10(-5)-10(-4) M. 5. The absorption peak at 610 nm is similar to that formed at high pH in the absence of bivalent cations and is due to ionization of phenolic groups with the dye molecule in an extended form. 6. The peak at 655 nm with 1:1 complex can be explained as a change in orientation of the diazo bonds caused by a conformational change of the molecule when it wraps around the single atom of Ca2+ or other large cation.

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Section: Ca2lmentioning

confidence: 99%

The interaction of cations with the dye arsenazo III

Rowatt

Williams

1989

Biochemical Journal

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“…7 Some well-known chromophores that change their absorbance in response to metal binding (metallochromic substances), such as Arsenazo III and Chlorophosphonazo III, exhibit specific absorbance changes in response to Ca 2+ . 8 However, these compounds have a lower selectivity for Ca 2+ as compared with BAPTA while forming complexes with two dye molecules per Ca 2+ . Furthermore, the negatively charged Ca 2+ -binding moieties of these dyes cannot be easily modified to achieve efficient delivery into cells.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, this set of specifications is not fulfilled by most current calcium sensors used for fluorescence microscopy, as those fluorophores, such as Oregon Green BAPTA-1, operate mainly by a change in QY . Some well-known chromophores that change their absorbance in response to metal binding (metallochromic substances), such as Arsenazo III and Chlorophosphonazo III, exhibit specific absorbance changes in response to Ca 2+ . However, these compounds have a lower selectivity for Ca 2+ as compared with BAPTA while forming complexes with two dye molecules per Ca 2+ .…”

mentioning

confidence: 99%

Calcium Sensor for Photoacoustic Imaging

Roberts¹,

Seeger²,

et al. 2017

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We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

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“…A common indicator used for complexometric titrations is Eriochrome Black T (EBT), which dissociates in water yielding anions having −1 e charge and a blue appearance 55 . However, when complexed with Ca 2+ , EBT has a pink appearance 56 . We perform a BCGE experiment demonstrating displacement in which the strong divalent-cation chelator ethylenediamine–tetraacetic acid 57 (EDTA 4− ) strips Ca 2+ from more weakly bound EBT–Ca complexes 58 .…”

Section: Resultsmentioning

confidence: 99%

Band-collision gel electrophoresis

Bikos

Mason

2019

Nat Commun

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Electrophoretic mobility shift assays are widely used in gel electrophoresis to study binding interactions between different molecular species loaded into the same well. However, shift assays can access only a subset of reaction possibilities that could be otherwise seen if separate bands of reagent species might instead be collisionally reacted. Here, we adapt gel electrophoresis by fabricating two or more wells in the same lane, loading these wells with different reagent species, and applying an electric field, thereby producing collisional reactions between propagating pulse-like bands of these species, which we image optically. For certain pairs of anionic and cationic dyes, propagating bands pass through each other unperturbed; yet, for other pairs, we observe complexing and precipitation reactions, indicating strong attractive interactions. We generalize this band-collision gel electrophoresis (BCGE) approach to other reaction types, including acid-base, ligand exchange, and redox, as well as to colloidal species in passivated large-pore gels.

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A survey of the available colorimetric indicators for Ca2+ and Mg2+ ions in biological experiments

Cited by 17 publications

References 20 publications

The interaction of cations with the dye arsenazo III

The interaction of cations with the dye arsenazo III

Calcium Sensor for Photoacoustic Imaging

Band-collision gel electrophoresis

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