A survey of sand flies (Diptera, Phlebotominae) along recurrent transit routes in Serbia

Vaselek, Slavica; Dvořák, Vít; Hlavačková, Kristýna; Ayhan, Nazlı; Halada, Petr; Oğuz, Gizem; Ivović, Vladimir; Özbel, Yusuf; Charrel, Rémi N.; Alten, Bülent; Petrić, Dušan

doi:10.1016/j.actatropica.2019.105063

Cited by 21 publications

(21 citation statements)

References 21 publications

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“…By nding a unique but genetically very close haplotype and a shared haplotype of COI and cyt b, respectively, to a haplotype from North Macedonia, post-glacial northward recolonization from this area seems likely. This is further corroborated by recent ndings in Serbia [37].…”

Section: Discussionsupporting

confidence: 87%

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Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Kniha

Dvořák

Milchram

et al. 2020

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Background Phlebotomine sand flies are the principal vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae). Sand fly findings in Central Europe are scarce and in Austria, to date only Phlebotomus mascittii has been recorded. In 2018 and 2019, entomological surveys were conducted in Austria with the aim to further clarify sand fly distribution and species composition. Results In 2019, a Ph. simici specimen was trapped in Austria for the first time. Analyses of two commonly used marker genes, COI and cyt b, revealed high sequence identity with Ph. simici specimens from North Macedonia and Greece. Phylogenetic analyses showed high intraspecific distances within Ph. simici, thereby dividing this species into three lineages, from Europe, Turkey and Israel, respectively. Low interspecific distances between Ph. simici, Ph. brevis and a yet unidentified Adlerius sp. from Turkey and Armenia highlights that molecular identification can be challenging within the Adlerius complex, even when applying standard marker genes. Conclusion This study provides the first finding of Ph. simici in Austria and the northernmost record so far. Moreover, it reveals valuable insights into the phylogenetic relationships of species within the Adlerius subgenus. Ph. simici is a suspected vector of Leishmania infantum and therefore of medical and veterinary importance. Potential sand fly expansion in Central Europe due to climatic change and the increasing import of Leishmania-infected dogs from endemic areas, urge the need for further studies on sand fly distribution in Austria and Central Europe in general.

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Section: Discussionsupporting

confidence: 87%

“…Recent reports from North Macedonia (Dvořák p.o. ), Kosovo [36] and Serbia [37] point towards a northward European distribution, which is further corroborated by an older mention from Croatia [38]. Ph.…”

Section: Discussionmentioning

confidence: 57%

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Kniha

Dvořák

Milchram

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…By nding a unique but genetically very close haplotype and a shared haplotype of coxI and cytb, respectively, to a haplotype from North Macedonia, post-glacial northward recolonization from this area seems likely. This is further corroborated by recent ndings in Serbia [32]. Temperatures in Central Europe during the Holocene optimum around 6,000 years ago were comparable to today and the presence of Mediterranean species in Central Europe may result from northward recolonization events from different refugial areas at that time [41].…”

Section: Discussionsupporting

confidence: 78%

“…Recent reports from North Macedonia (Dvořák p.o. ), Kosovo [31] and Serbia [32] point towards a northward European distribution, which is further corroborated by an older mention from Croatia [33]. Ph.…”

Section: Discussionmentioning

confidence: 57%

Phlebotomus (Adlerius) simici NITZULESCU, 1931: first record in Austria and phylogenetic relationship with other Adlerius species 

Kniha

Dvořák

Milchram

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Phlebotomine sand flies are the principal vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae). Sand fly findings in Central Europe are scarce and in Austria, to date only Phlebotomus mascittii has been recorded. In 2018 and 2019, entomological surveys were conducted in Austria with the aim to further clarify sand fly distribution and species composition.Results: In 2019, a Ph. simici specimen was trapped in Austria for the first time. Analyses of two commonly used marker genes, coxI and cytb, revealed high sequence identity with Ph. simici specimens from North Macedonia and Greece. Phylogenetic analyses showed high intraspecific distances within Ph. simici, thereby dividing this species into three lineages, from Europe, Turkey and Israel, respectively. Low interspecific distances between Ph. simici, Ph. brevis and a yet unidentified Adlerius sp. from Turkey and Armenia highlights that molecular identification can be challenging within the Adlerius complex, even when applying standard marker genes.Conclusion: This study provides the first finding of Ph. simici in Austria and the northernmost record so far. Moreover, it reveals valuable insights into the phylogenetic relationships of species within the Adlerius subgenus. Ph. simici is a suspected vector of Leishmania infantum and therefore of medical and veterinary importance. Potential sand fly expansion in Central Europe due to climatic change and the increasing import of Leishmania-infected dogs from endemic areas, urge the need for further studies on sand fly distribution in Austria and Central Europe in general.

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“…Collective field studies in the Mediterranean region [ 7 ] and neighboring countries like Albania [ 8 ] and Serbia [ 9 , 10 ], verified the presence of several Phlebotomus species like P. neglectus, P. tobbi, P. balkanicus, P. ariasi, P. perfiliewi, P. perniciosus, P. kandelakii, P. mascitii, P. alexandri, P. sergenti. P. similis, P. simici, P. patasi, all being proven or potential vectors of Leishmania .…”

Section: Introductionmentioning

confidence: 99%

Host Species Determines the Composition of the Prokaryotic Microbiota in Phlebotomus Sandflies

et al. 2020

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Phlebotomine sandflies are vectors of the humans’ and mammals’ parasite Leishmania spp. Although the role of gut microbiome in the biological cycle of insects is acknowledged, we still know little about the factors modulating the composition of the gut microbiota of sandflies. We tested whether host species impose a strong structural effect on the gut microbiota of Phlebotomus spp. Sandflies were collected from the island of Leros, Greece, and classified to P. papatasi, P. neglectus, P. tobbi, and P. similis, all being negative to Leishmania spp. The prokaryotic gut microbiota was determined via 16S rRNA gene amplicon sequencing. Phlebotomus species supported distinct microbial communities (p < 0.001). P. papatasi microbiota was the most distinct over-dominated by three Spiroplasma, Wolbachia and Paenibacillus operational taxonomic units (OTUs), while another Wolbachia OTU prevailed in P. neglectus. Conversely, the microbiota of P. tobbi and P. similis was composed of several less dominant OTUs. Archaea showed low presence with the dominant OTUs belonging to methanogenic Euryarcheota, ammonia-oxidizing Thaumarcheota, and Nanoarchaeota. We provide first insights into the composition of the bacterial and archaeal community of Phlebotomus sandflies and showed that, in the absence of Leishmania, host genotype is the major modulator of Phlebotomus sandfly gut microbiota.

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A survey of sand flies (Diptera, Phlebotominae) along recurrent transit routes in Serbia

Cited by 21 publications

References 21 publications

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Phlebotomus (Adlerius) simici NITZULESCU, 1931: first record in Austria and phylogenetic relationship with other Adlerius species

Host Species Determines the Composition of the Prokaryotic Microbiota in Phlebotomus Sandflies

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A survey of sand flies (Diptera, Phlebotominae) along recurrent transit routes in Serbia

Cited by 21 publications

References 21 publications

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Phlebotomus (Adlerius) simici NITZULESCU, 1931: first record in Austria and phylogenetic relationship with other Adlerius species&nbsp;

Host Species Determines the Composition of the Prokaryotic Microbiota in Phlebotomus Sandflies

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Phlebotomus (Adlerius) simici NITZULESCU, 1931: first record in Austria and phylogenetic relationship with other Adlerius species