Abstract. This paper has investigated the extensive implementation of distinct types of pipes in the Water Distribution System (WDS) and evaluated the impacts of particular leachable organic chemicals and bacteriological issues. Besides, the paper inspects significant parameters of water quality as the population of Rajshahi City, Bangladesh relies on water provided via pipes for drinking and other domestic purposes. This study aims to assess the quality of physical, chemical, and microbiological parameters of supplied drinking water through lines in Rajshahi City Corporation (RCC) by Rajshahi Water Supply and Sewerage Authority (RWASA). Therefore, the study managed a total of sixteen physical, chemical, and microbiological parameters to analyse them in the laboratory. The experimental results showed that pH and hardness of all samples were within the allowable limit as per Bangladesh Drinking Water Standards (BDWSs) and World Health Organization (WHO). All models contained an extreme level of iron and manganese. They also included a negligible amount of arsenic. The experiment detected lesser Dissolved Oxygen (DO), Residual Chlorine (Residual Cl), and the undesirable odour in about 90 % samples. All samples contained Total Coliform (TC) and Escherichia coli (E. coli) bacteria. A few samples contained a significant amount of turbidity, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), and Electrical Conductivity (EC). The authors developed a statistical analysis by SPSS software to co-relate the parameters. This study recommends the presence of such bacteria, iron, and manganese in the pipeline.