A Survey of Aminoacidemias with Special Reference to the High Concentration of Free Aminoacids in Insect Hemolymph

Duchateau, G; Florkin, Marcel

doi:10.3109/13813455809084234

Cited by 49 publications

(4 citation statements)

References 12 publications

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“…Protein quantifications was measured by Bradford dye‐binding method () with coomassie brilliant blue (G 250; Merck, Germany) using reagent and bovine serum albumin (Sigma, St Louis, Missouri) as standard. Carbohydrates content were determined according to Duchateau & Florkin () using anthrone reagent and glucose as standard. Lipids were measured by the vanillin method (Goldsworthy et al ., ) and the table oil Afia was used as a standard.…”

Section: Methodsmentioning

confidence: 99%

Activity of a selective insecticide (methoxyfenozide) against two mosquito species (Culex pipiensandCuliseta longiareolata): toxicological, biometrical and biochemical study

2018

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Methoxyfenozide is an insect growth disruptor belonging to the class of nonsteroidal ecdysone agonists. In the present study, methoxyfenozide (23% emulsifiable concentrate) is evaluated against newly molted fourth‐instar larvae of Culex pipiens L. and Culiseta longiareolata Macquart (Diptera: Culicidae), aiming to investigate its possible effects on growth and development. Larvae are exposed for 24 h under standard laboratory conditions in accordance with World Health Organization recommendations. The product is found to exhibit insecticidal activity against the two tested mosquito species with a concentration–response relationship. Moreover, based on the lethal concentrations determined, methoxyfenozide is slightly more toxic against C. pipiens than C. longiareolata. Furthermore, fourth‐instar larva of the two mosquito species are treated using two lethal concentrations (LC50 and LC90) of methoxyfenozide, and then individual survivals (larvae, pupae and adults) from larval treatment are subjected to a biometrical and biochemical study. The compound is found to interfere with growth by reducing the larval and pupal development duration. Moreover, the body volume and the main biochemical contents (proteins, carbohydrates and lipids) from different stages are affected. The overall results suggest that the ecdysone agonist under investigation interferes with the development process and has potential for mosquito control.

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Section: Methodsmentioning

confidence: 99%

Activity of a selective insecticide (methoxyfenozide) against two mosquito species (Culex pipiensandCuliseta longiareolata): toxicological, biometrical and biochemical study

2018

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“…Free amino acids composition for many species have been reviewed (Duchateau & Florkin, 1958;and He & Zhang, 2017). Amino acids have long been recognized to have a high concentration in insects, but differ in its composition according to studied insect tissue (Sankar & Yogamoorthi, 2012); insect and the insect stage (Haag & Sullivan, 1984 The obtained results also indicated that glutamic acid and aspartic acid were the most predominant FAAs.…”

Section: F Results and Discussionmentioning

confidence: 94%

Free amino acids composition and its changes at developmental stages of pink bollworm, Pectinophora gossypiella

Ahmed

2020

Egyptian Journal of Agricultural Research

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ree amino acid has an important role in physiological functions. Pink bollworm (PBW), Pectinophora gossypiella, was investigated for the free amino acid (FAAs) composition at immature stages (i.e., egg, larvae and pupae). Seventeen AAs were distinguished in the hole body of the different immature PBW stages. Essential AAs of tested stages of PBW consisted of threonine (THR), valine (VAL), methionine (MET), isoleucine (ILE), leucine (LEU), phenylalanine (PHE), lysine (LYS). Semi-essential AAs were histidine (HIS), arginine (ARG), cysteine (CYS), while non-essential AAs were aspartic acid (ASP), serine (SER), glutamic acid (GLU), glycine (GLY), alanine (ALA), tyrosine (TYR), and proline (PRO).The most prevailing FAAs, which exceeded 2 mg/100 mg, were LEU, LYS, ARG, ASP, GLU, ALA and TYR in all stages. Another AAs joined this group: VAL in larval stage, whereas its concentration was 2.28 mg/100 mg and exceeded that in egg by 16.92% and PHE & PRO in pupal stage, where it increased to 2.19 and 2.07 mg/100 mg, respectively. Also, in other words GLU was the most abundant FAAs in the egg stage (4.46%), while ASP and GLU were the most abundant FAAs in the larval and pupal stages 3.19 & 3.35% for ASP and 4.24 and 4.14% for GLU, respectively. On the other hand, CYS is the lowest abundant one recorded 0.68, 0.55 and 0.64% for egg, larvae and pupae, respectively). Furthermore, no qualitative changes in the FAAs composition of different immature stages of PBW, but there were quantitative changes. All FAAs increased from stage to other except for MET, CYS, SER, GLU and GLY, and the decreasing continued until pupal stage in the case of GLU, where it recorded 4.46, 4.24 and 4.14 mg/100mg in egg, larval and pupal stage, respectively. FAAs concentrations in egg were 1.21, 0.68, 1.78 and 1.82 mg/100mg for MET, CYS, SER, and GLY, respectively. These values decreased when PBW morphing from egg to larvae by 14.88, 19.12, 23.03 and 19.23%, respectively. After that, the change in FAAs concentration switch to increasing but still low from that recorded in egg in the case of CYS, SER & GLY represented 0.64, 1.52 & 1.50 mg/100mg, respectively. While, in MET the increasing in FAAs concentration when PBW morphing from egg to larvae continues until pupal stage and exceeded the value recorded in egg by 5.79% and 24.27% in larvae.

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“…Имеются данные о прямой корреляции между количеством глицерина в насекомых и холодостойкостью вида (Salt, 1961;Merivee и др., 1968). В то же время отмечено, что гемолимфа диапаузирующих куколок многих представителей вида чешуекрылых содержит по сравнению с недиапаузирующими куколками необычно большие количества некоторых аминокислот (Duchäteu, Florkin, 1958). Норвежский исследователь Л. Сэмме (Somme, 1967) показал существование прямой корреляции между температурой, при которой содержались гусеницы Nemapogon personellus и куколки Pies brassiсае, аноксией и содержанием в них трегалоза и аланина, сорбита и аланина соответственно.…”

Section: диапаузаunclassified

Содержание Свободных Аминокислот У Гусениц Соснового Почкового Побеговьюна (Blastesthia Тurionella L.).

Veimer¹

1972

Proceedings of the Academy of Sciences of the Estonian SSR. Bio

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Free amino acids were identified by paper chromatography. Their concentration was determined in diapausing larvae and in post-diapausing developing larvae.Nine amino acids were found in both cases. The level of alanin and prolin was found to be higher in diapausing forms than in post-diapause developing ones.The higher level of these amino acids in diapausing larvae appears to be of secondary nature and indirectly associated with the high cold-hardiness of Blastesthia turionella.Academy of Sciences of the Estonian SSR,

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A Survey of Aminoacidemias with Special Reference to the High Concentration of Free Aminoacids in Insect Hemolymph

Cited by 49 publications

References 12 publications

Activity of a selective insecticide (methoxyfenozide) against two mosquito species (Culex pipiensandCuliseta longiareolata): toxicological, biometrical and biochemical study

Activity of a selective insecticide (methoxyfenozide) against two mosquito species (Culex pipiensandCuliseta longiareolata): toxicological, biometrical and biochemical study

Free amino acids composition and its changes at developmental stages of pink bollworm, Pectinophora gossypiella

Содержание Свободных Аминокислот У Гусениц Соснового Почкового Побеговьюна (Blastesthia Тurionella L.).

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