A surface plasmon resonance based biochip for the detection of patulin toxin

Pennacchio, Anna; Ruggiero, Giuseppe; Staiano, Maria; Piccialli, Gennaro; Oliviero, Giorgia; Lewkowicz, Aneta; Synak, Anna; Bojarski, Piotr; D’Auria, Sabato

doi:10.1016/j.optmat.2013.12.045

Cited by 53 publications

(22 citation statements)

References 20 publications

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“…A competitive surface plasmon resonance (SPR) based bioassay for patulin detection was proposed by Pennacchio et al (2014). In this work, the self-prepared patulin derivative…”

Section: Ce Methodsmentioning

confidence: 99%

Determination of trace patulin in apple-based food matrices

Zhang

2017

Food Chemistry

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“…A competitive surface plasmon resonance (SPR) based bioassay for patulin detection was proposed by Pennacchio et al (2014). In this work, the self-prepared patulin derivative…”

Section: Ce Methodsmentioning

confidence: 99%

Determination of trace patulin in apple-based food matrices

Zhang

2017

Food Chemistry

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“…After the immunization period, the rabbits were sacrificed, and blood was recovered and centrifuged to separate blood cells from serum. A sample of rabbit serum was applied to a Protein A column of the PURE 1A Protein A Antibody Purification Kit, and the IgG fraction was purified according to Pennacchio (Pennacchio et al, 2014). The elution of proteins was monitored by absorbance at k = 278 nm.…”

Section: Antibody Production and Purificationmentioning

confidence: 99%

“…For affinity chromatography purification, an aliquot of the IgG fraction was applied drop-wise to the prepared affinity column. To remove compounds bound un-specifically, the column was washed with three high-salt buffers, according to Pennacchio et al (2014). For the elution step, glycine (0.1 mol/L) at pH 2.8 (2.5 mL) was applied to the column.…”

Section: Affinity Column Preparation Of Penicillin G-eah Sepharose 4bmentioning

confidence: 99%

A novel fluorescence polarization assay for determination of penicillin G in milk

et al. 2016

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“…Despite the widespread development of immunoassays for other mycotoxins, the development of such assays for PAT has been limited by the absence of good antibodies to this toxin. However, recently several immunoassays have been described for PAT (de Champdore et al 2007;Pennacchio et al 2014;Funari et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA)

Maragos

Busman

et al. 2015

Food Additives & Contaminants: Part A

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Patulin is a mycotoxin commonly found in certain fruit and fruit products. For this reason many countries have established regulatory limits pertaining to, in particular, apple juice and apple products. Fruit leathers are produced by dehydrating fruit puree, leaving a sweet product that has a leathery texture. A recent report in the literature described the detection of patulin at substantial levels in fruit leathers. To investigate this further, an ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA) method was developed for the sensitive detection of patulin in fruit leathers. Investigations were also made of the suitability of direct analysis in real time-mass spectrometry (DART-MS) for detection of patulin from the surface of fruit leathers. Results indicated DART-MS was insufficiently sensitive for quantification from the surface of home-style apple leathers, although patulin spiked onto the surface of leather or peel could be detected. The UPLC-PDA method was used to determine the fate of patulin during the preparation of home-made fruit leathers. Interestingly, when a home-style process was used, the patulin was not destroyed, but rather increased in concentration as the puree was dehydrated. The UPLC-PDA method was also used to screen for patulin in commercial fruit leathers. Of the 36 products tested, 14 were above the limit of detection (3.5 μg kg(-1)) and nine were above the limit of quantification (12 μg kg(-1)). Positive samples were confirmed by UPLC-MS/MS. Only one sample was found above the US regulatory limit for single-strength apple juice products (50 μg kg(-1)). These results suggest patulin can be concentrated during preparation and can be found in fruit leathers. The limited survey suggests that patulin is fairly prevalent in such commercial products, but that the levels are usually low.

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A surface plasmon resonance based biochip for the detection of patulin toxin

Cited by 53 publications

References 20 publications

Determination of trace patulin in apple-based food matrices

Determination of trace patulin in apple-based food matrices

A novel fluorescence polarization assay for determination of penicillin G in milk

Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA)

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