A surface 75-kDa protein with acid phosphatase activity recognized by monoclonal antibodies that inhibit Paracoccidioides brasiliensis growth

Xander, Patrícia; Vigna, Ana Flávia; Feitosa, Luciano dos Santos; Pugliese, Lívia Souza; Bailão, Alexandre Melo; Soares, Célia Maria de Almeida; Mortara, Renato Arruda; Mariano, Mário; Lopes, José Daniel

doi:10.1016/j.micinf.2007.08.001

Cited by 32 publications

(33 citation statements)

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“…Treatment with specific mAb affected in vitro fungal growth. Thus, passive immunization with mAb preceding P. brasiliensis infection in mice drastically reduced fungal burden and inflammation compared with the control [10,28]. Analysis of the peptide sequence of the gp70 molecule from S. schenckii shows significant homology with a 89-kDa major surface glycoprotein 110 of P. carinii (msg 110).…”

Section: Discussionmentioning

confidence: 99%

“…Treatment with specific mAb affected in vitro fungal growth. Thus, passive immunization with mAb preceding P. brasiliensis infection in [10,28].…”

Section: Discussionmentioning

confidence: 99%

“…Human antibodies against glucosylceramide from C. neoformans inhibited cell budding and fungal growth probably by interfering with the biosynthesis and organization of the cell-wall polymers [27]. Recently, Xander et al [28] reported a characterization of a secreted 75-kDa protein from P. brasiliensis with phosphatase activity [28]. Treatment with specific mAb affected in vitro fungal growth.…”

mentioning

confidence: 99%

“…Treatment with specific mAb affected in vitro fungal growth. Thus, passive immunization with mAb preceding P. brasiliensis infection in [10,28].Analysis of the peptide sequence of the gp70 molecule from S. schenckii shows significant homology with a 89-kDa major surface glycoprotein 110 of P. carinii (msg 110). Some authors have reported that the msg 110 from P. carinii is involved in attachment to epithelial cells [29][30][31].…”

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confidence: 99%

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Passive immunization with monoclonal antibody against a 70‐kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis

et al. 2008

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Cell-mediated and innate immunity are considered the most important mechanisms of host defense against fungus infections. However, recent studies demonstrated that specific antibodies show different degrees of protection against mycosis. In a previous study, antigens secreted by Sporothrix schenckii induced a specific humoral response in infected animals, mainly against the 70-kDa molecule, indicating a possible participation of antibodies to this antigen in infection control. In the present study, an IgG1 mAb was produced against a 70-kDa glycoprotein of S. schenckii in order to better understand the effect of passive immunization of mice infected with S. schenckii. Results showed a significant reduction in the number of CFU in organs of mice when the mAb was injected before and during S. schenckii infection. Similar results were observed when T-cell-deficient mice were used. Moreover, in a second schedule treatment, the mAb was injected after infection was established, and again we observed a significant reduction in CFU associated with an increase of IFN-cproduction. Also, the 70-kDa antigen is shown to be a putative adhesin present on the surface of this fungus. In conclusion, we report for the first time the protective effect of a specific antibody against S. schenckii.Key words: Adhesins . Mab . Passive immunization . Sporothrix schenckii . Sporotrichosis IntroductionSporotrichosis is a chronic subcutaneous mycosis that occurs worldwide. However, this disease is commonly seen in tropical and subtropical regions [1]. It is caused by traumatic inoculation of the dimorphic fungus Sporothrix schenckii, which is an ubiquitous environmental saprophyte that can be isolated from soil and plant debris [2]. In the past, infections were limited to the cutaneous forms of the disease.Recently, occurrences of more severe clinical forms of this mycosis were described, especially among immunocompromised individuals [3,4].The immunological mechanisms involved in prevention and control of sporotrichosis suggest that cell-mediated immunity plays an important role in protecting The host against S. schenckii [5]. In experimental infections, athymic nude mice are more susceptible to sporotrichosis, and acquired immunity against S. schenckii is mainly mediated by T-cell-activated macrophages [6,7].In contrast, the role of the humoral immune response in protection against this fungus has not been studied in detail. In a previous study, we showed that antigens secreted by S. 3080induce a specific humoral response in infected animals, mainly against the 70-kDa molecule, indicating a possible participation of specific antibodies to this molecule in infection control [8].Cell-mediated and innate immunity are considered the most important mechanisms of host defense against fungus infections. However, recent studies demonstrated that antibodies with defined specificity show different degrees of protection against mycosis [9]. Administration of mAb-mediated protection against Paracoccidioides brasiliensis and Candida albicans and modif...

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Section: Discussionmentioning

confidence: 99%

“…Treatment with specific mAb affected in vitro fungal growth. Thus, passive immunization with mAb preceding P. brasiliensis infection in [10,28].…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Passive immunization with monoclonal antibody against a 70‐kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis

et al. 2008

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“…Recently, several studies have established that some antibodies are protective against fungi (31,41,52). MAbs or fragments of MAbs have been approved for the clinical evaluation of cryptococcosis (21) and candidiasis (32).…”

Section: Discussionmentioning

confidence: 99%

Avaliação do peptídeo sintético (P10): associado ao tratamento quimioterápico em camundongos BALB/c anérgicos infectados com Paracoccidioides brasiliensis.

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Therapeutic and Prophylactic Tools for Sporotrichosis: Current Strategies and Future Tendencies

et al. 2015

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A surface 75-kDa protein with acid phosphatase activity recognized by monoclonal antibodies that inhibit Paracoccidioides brasiliensis growth

Cited by 32 publications

References 27 publications

Passive immunization with monoclonal antibody against a 70‐kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis

Passive immunization with monoclonal antibody against a 70‐kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis

Avaliação do peptídeo sintético (P10): associado ao tratamento quimioterápico em camundongos BALB/c anérgicos infectados com Paracoccidioides brasiliensis.

Therapeutic and Prophylactic Tools for Sporotrichosis: Current Strategies and Future Tendencies

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