A Supramolecular‐Hydrogel‐Encapsulated Hemin as an Artificial Enzyme to Mimic Peroxidase

Wang, Qigang; Yang, Zhimou; Zhang, Xieqiu; Xiao, Xudong; Chang, C. K.; Xu, Bing

doi:10.1002/anie.200700404

Cited by 375 publications

(273 citation statements)

References 61 publications

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“…This paper describes the evaluation of the catalytic activity of artificial enzymes composed of a supramolecular hydrogel [1,2,3] and a group of synthetic heme model compounds. [4,5,6] It is well-known that the spatial arrangement of atoms or groups near the active center determines the activities of hemoproteins.…”

Section: Introductionmentioning

confidence: 99%

High Catalytic Activities of Artificial Peroxidases Based on Supramolecular Hydrogels That Contain Heme Models

Wang

Yang

et al. 2008

Chemistry A European J

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Composed of a supramolecular hydrogel and a heme model compound, a new type of artificial peroxidase shows high catalytic activity in organic media. The activity of this new type of artificial enzyme is significantly higher than that of the heme model compounds alone. Changes in the distal substituents above the coordinated-metal centers of the model compounds directly modulate catalytic activity. This supramolecular-hydrogel-based artificial enzyme is most active in toluene, reaching about 90% of the nascent activity of horseradish peroxidase. Moreover, this study confirms that the incorporation of the heme models into the nanofibers of gelators accounts for most of the enhancement of catalytic activity.

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Section: Introductionmentioning

confidence: 99%

High Catalytic Activities of Artificial Peroxidases Based on Supramolecular Hydrogels That Contain Heme Models

Wang

Yang

et al. 2008

Chemistry A European J

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“…Artificial mimicking enzymes possessing high catalytic activity and distinct substrate selectivity are highly desired for developing new catalytic reactions and bioanalysis systems due to their simpler syntheses and preparation, better stability, and easier modification than natural enzymes [38]. Therefore, lots of researches have focused on the development of enzyme mimetics-based signal amplification for immunosensing using coordination compounds with peroxidase activity, such as hemin and porphyrin, and catalytic/electroactive nanoparticles, especially metal and semiconductor nanoparticles as labels [39][40][41][42][43].…”

Section: Enzyme Mimics For Signal Amplificationmentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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To dissolve the bottleneck problem of life and biomedical science in detection of biomolecules with low abundance and acquisition of ultraweak biological signals, highly sensitive analytical methods coupling with the specificity of biological recognition events have been quickly developed by the exploring of signal amplification strategies. These strategies have extensively been introduced into the development of highly sensitive immunosensing methods by combining with highly specific immunological recognition. They can be divided into two groups. One group of strategies attempts to transfer the immunological recognition event into large number of reporter probes or signal probes for signal readout by employing nano/micro-materials as vehicles for multi-labeling and/or molecular biological amplification for increasing the abundance of the signal molecules. The other uses nanomaterials or enzyme mimics as catalytic tools/tags to obtain enhanced detection signal. This review focuses on the significant advances in design of signal amplification strategies for highly sensitive immunosensing.

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“…Strong p-p interactions between hemin molecules and the nanofibers of 1 and 2 might explain why the quantum yield is lower than that of Gel 1 + 2 A C H T U N G T R E N N U N G [3, 4a]. [9,18] According to the kinetics of the chemiluminescent reaction, CL intensity is related to the reaction rate k via the quantum yield. In the kinetic curve, k is a multicomponent variable determined by the changing luminol concentration due to the continued consumption and the deactivation of 4, while k is a single component in a quasi-steady enzymatic reaction.…”

Section: Introductionmentioning

confidence: 99%

“…[4] Several notable results support this choice: i) research on macromolecular crowding has shown that nanoscale confinement of reactions can play an essential role in cells; [5] ii) fluorophores within nanofibers show significantly enhanced fluorescence compared to that in an ordinary solution; [6] iii) the encapsulation of enzymes within nanofibers in a hydrogel can substantially improve their activity and the stability. [7,8,9] To demonstrate the concept, luminescence from the oxidation of luminol by H 2 O 2 , which can be catalyzed by heme proteins, [10] was studied. This process was selected because it is a well-established chemiluminescent reaction with many applications.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Supramolecular Confinement of Luminol and Heme Proteins to Enhance the Chemiluminescent Quantum Yield

Wang

2009

Chemistry A European J

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Artificial biolumiscence: The use of a supramolecular hydrogel to confine heme proteins and luminol can enhance the quantum yield of chemiluminescence (CL) by about an order of magnitude and give an emission time 4-6 times longer.Confining heme proteins and luminol with the nanofibers of self-assembled molecules was shown to enhance the quantum yield of their chemiluminescence by about an order of magnitude. Supramolecular hydrogels formed using (9-fluorenylmethyl)carbamate (Fmoc) peptides mimicked the cellular environment of bioluminescence, which typically shows high quantum yield. The effective emission times observed were 4-6 times longer and were ascribed to the stability of the heme proteins in the hydrogel, as confirmed by quantitative analysis. This chemiluminescent system was applied to detecting trace levels of blood with 4-6 times enhancement of intensity and better sensitivity for the highly diluted sample, indicating that this approach might be extended to other applications of chemiluminescent analysis, including immunoassays.

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A Supramolecular‐Hydrogel‐Encapsulated Hemin as an Artificial Enzyme to Mimic Peroxidase

Cited by 375 publications

References 61 publications

High Catalytic Activities of Artificial Peroxidases Based on Supramolecular Hydrogels That Contain Heme Models

High Catalytic Activities of Artificial Peroxidases Based on Supramolecular Hydrogels That Contain Heme Models

Signal Amplification for Highly Sensitive Immunosensing

Bioinspired Supramolecular Confinement of Luminol and Heme Proteins to Enhance the Chemiluminescent Quantum Yield

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