A <sup>1</sup>H‐NMR method for determining temperature in cell culture perfusion systems

Lutz, Norbert W.; Kuesel, Annette C.; Hull, William E.

doi:10.1002/mrm.1910290120

Cited by 62 publications

(50 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro studies have demonstrated that this coefficient remains practically independent of tissue type (31) and pH (32). Although a precise relationship between the water proton NMR frequency and temperature for in vivo brain tissue is yet to be established, preliminary data in cell cultures (32) and different biological tissues (31) indicate that this dependence is close to that cited above for pure water. In our experiments, the frequency of the water signal was determined by fitting of the phase of the complex MR signal to a quadratic polynomial:…”

Section: Mr Techniquementioning

confidence: 64%

“…The frequency of the MR signal of water depends on temperature and changes with a coefficient of about Ϫ0.01 ppm͞°C (21). In vitro studies have demonstrated that this coefficient remains practically independent of tissue type (31) and pH (32). Although a precise relationship between the water proton NMR frequency and temperature for in vivo brain tissue is yet to be established, preliminary data in cell cultures (32) and different biological tissues (31) indicate that this dependence is close to that cited above for pure water.…”

Section: Mr Techniquementioning

confidence: 99%

See 1 more Smart Citation

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Yablonskiy

Ackerman

Raichle

2000

Proc. Natl. Acad. Sci. U.S.A.

209

208

View full text Add to dashboard Cite

CorrectionsNEUROBIOLOGY. For the article ''Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation'' by Dmitriy A. Yablonskiy, Joseph J. H. Ackerman, and Marcus E. Raichle, which appeared in number 13, June 20, 2000, of Proc. Natl. Acad. Sci. USA (97, 7603-7608), the authors note a correction in the second to last paragraph on page 7604. The sentence ''It is well known from positron-emission tomography (PET) data (17, 18) that rCMRO 2 maps in the normal human brain are largely flat despite considerable regional differences in rCBF and rCMRO 2 maps'' should read: ''It is well known from positron-emission tomography (PET) data (17,18) A fundamental discovery of modern human brain imaging with positron-emission tomography that the blood flow to activated regions of the normal human brain increases substantially more than the oxygen consumption has led to a broad discussion in the literature concerning possible mechanisms responsible for this phenomenon. Presently no consensus exists. It is well known that oxygen delivery is not the only function of systemic circulation. Additional roles include delivery of nutrients and other required substances to the tissue, waste removal, and temperature regulation. Among these other functions, the role of regional cerebral blood flow in local brain temperature regulation has received scant attention. Here we present a theoretical analysis supported by empirical data obtained with functional magnetic resonance suggesting that increase in regional cerebral blood flow during functional stimulation can cause local changes in the brain temperature and subsequent local changes in the oxygen metabolism. On average, temperature decreases by 0.2°C, but individual variations up to ؎1°C were also observed. Major factors contributing to temperature regulation during functional stimulation are changes in the oxygen consumption, changes in the temperature of incoming arterial blood, and extensive heat exchange between activated and surrounding brain tissue.

show abstract

Section: Mr Techniquementioning

confidence: 64%

Section: Mr Techniquementioning

confidence: 99%

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Yablonskiy

Ackerman

Raichle

2000

Proc. Natl. Acad. Sci. U.S.A.

209

208

View full text Add to dashboard Cite

show abstract

“…The index ␣ is the temperature-dependent coefficient of the water chemical shift in units of ppm/°C (or equivalently, ppm/K). It is well known that ␣ for the pure water is about -0.01 ppm/°C (ppm/K) (14) and it is almost independent of tissue compositions (15). Owing to linear and stable temperature sensitivity, the PRF shift method appears to be a reliable method for monitoring temperature change during thermal therapy (12).…”

mentioning

confidence: 99%

Simultaneous temperature and magnetization transfer (MT) monitoring during high‐intensity focused ultrasound (HIFU) treatment: Preliminary investigation on ex vivo porcine muscle

Peng

Huang

Tseng

et al. 2009

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose:To measure temperature change and magnetization transfer ratio (MTR) simultaneously during high-intensity focused ultrasound (HIFU) treatment. Materials and Methods:This study proposed an interleaved dual gradient-echo technique to monitor the heat and tissue damage brought to the heated tissue. The technique was applied to tissue samples to test its efficacy. Results:Ex vivo experiments on the porcine muscle demonstrated that both temperature changes and MTR exhibited high consistency in localizing the heated regions. As the heat dissipated after the treatment, the temperature of the heated regions decreased rapidly but MTR continued to be elevated. Moreover, thermal dose (TD) maps derived from the temperature curves demonstrated a sharp margin in the heated regions, but MTR maps may show a spatial gradient of tissue damage, suggesting complimentary information provided by these two measures.Conclusion:In a protocol of spot-by-spot heating over a large volume of tissue, MTR provides additional values to mark the locations of previously heated regions. By continuously recording the locations of heated spots, MTR maps could help plan the next target spots appropriately, potentially improving the efficiency of HIFU treatment and reducing undesirable damage to the normal tissue. RECENT DEVELOPMENT OF HIGH-INTENSITY FO-CUSED ULTRASOUND (HIFU) technology has offered a potentially new approach to the local ablation of malignant or benign tumors (1,2). By focusing an ultrasound beam at the target tissue, HIFU maximizes the heating damage to the targeted tissue while preserving the surrounding normal tissue. To improve treatment efficiency and ensure patient safety, it is crucial to perform continuous and real-time monitoring of local heat deposition during HIFU treatment. Owing to satisfactory spatial and temporal resolution, high tissue contrast, and no ionizing radiation, MRI is advantageous over other imaging modalities in guiding, monitoring, and controlling the HIFU treatment, and has therefore been used for preheating localization of the targets or postheating evaluation of the treatment effect (3). Several MR parameters, such as the equilibrium magnetization (M 0 ), T1, T2, and the diffusion coefficient of water molecules, are known to exhibit temperature dependence (4 -10). The temperature sensitivity of these parameters, however, is tissue-dependent, nonlinear, or varies with tissue conditions such as thermal coagulation or denaturation (5,11,12). Currently, the MR parameter that is sensitive to temperature and stable under various tissue conditions is the water proton resonant frequency (PRF) shift (5). The method of PRF shift deter-

show abstract

“…Several noninvasive thermometers employing NMR techniques have been proposed based on the temperature dependence of NMR parameters, such as relaxation time (30), diffusion coefficient (10), chemical shift (2,6,8,20), or the proton frequency method (11,15,19). Reliability with an error range of less than Ϯ1°C is required for application of these techniques to the estimation of the temperature in the human body under physiological conditions (21,23,32,33).…”

mentioning

confidence: 99%

Noninvasive measurement of temperature and fractional dissociation of imidazole in human lower leg muscles using ¹H-nuclear magnetic resonance spectroscopy

Yoshioka

Ehara²,

Inoue³

et al. 2005

Journal of Applied Physiology

View full text Add to dashboard Cite

The temperature change of the fractional dissociation of imidazole (alpha-imidazole) in resting human lower leg muscles was measured noninvasively using (1)H-nuclear magnetic resonance spectroscopy at 3.0 and 1.5 T on five normal male volunteers aged 30.6 +/- 10.4 yr (mean +/- SD). Using (1)H-nuclear magnetic resonance spectroscopy, water, carnosine, and creatine in the muscles could be simultaneously analyzed. Carnosine contains imidazole protons. The chemical shifts of water and carnosine imidazole protons relative to creatine could be used for estimating temperatures and alpha-imidazole, respectively. Using the chemical shift, the values of temperature in gastrocnemius (Gast) and soleus muscles at ambient temperature (21-25 degrees C) were estimated to be 35.5 +/- 0.5 and 37.4 +/- 0.6 degrees C (means +/- SE), respectively (significantly different; P < 0.01). The estimated values of alpha-imidazole in these muscles were 0.620 +/- 0.007 and 0.630 +/- 0.013 (means +/- SE), respectively (not significant). Alternation of the surface temperature of the lower leg from 40 to 10 degrees C significantly changed the temperature in Gast (P < 0.0001) from 38.1 +/- 0.5 to 28.0 +/- 1.2 degrees C, and the alpha-imidazole in Gast decreased from 0.631 +/- 0.003 to 0.580 +/- 0.011 (P < 0.05). However, the values of alpha-imidazole and the temperature in soleus muscles were not significantly affected by this maneuver. These results indicate that the alpha-imidazole in Gast changed significantly with alternation in muscle temperature (r = 0.877, P < 0.00001), and its change was estimated to be 0.0058/ degrees C.

show abstract

A ¹H‐NMR method for determining temperature in cell culture perfusion systems

Cited by 62 publications

References 9 publications

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Simultaneous temperature and magnetization transfer (MT) monitoring during high‐intensity focused ultrasound (HIFU) treatment: Preliminary investigation on ex vivo porcine muscle

Noninvasive measurement of temperature and fractional dissociation of imidazole in human lower leg muscles using ¹H-nuclear magnetic resonance spectroscopy

Contact Info

Product

Resources

About

A 1H‐NMR method for determining temperature in cell culture perfusion systems

Cited by 62 publications

References 9 publications

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation

Simultaneous temperature and magnetization transfer (MT) monitoring during high‐intensity focused ultrasound (HIFU) treatment: Preliminary investigation on ex vivo porcine muscle

Noninvasive measurement of temperature and fractional dissociation of imidazole in human lower leg muscles using 1H-nuclear magnetic resonance spectroscopy

Contact Info

Product

Resources

About

A ¹H‐NMR method for determining temperature in cell culture perfusion systems

Noninvasive measurement of temperature and fractional dissociation of imidazole in human lower leg muscles using ¹H-nuclear magnetic resonance spectroscopy