A suction-type, pneumatic microfluidic device for liquid transport and mixing

Weng, Cheng‐I; Lien, Kang-Yi; Yang, Sung-Yi; Lee, Gwo‐Bin

doi:10.1007/s10404-010-0669-1

Cited by 73 publications

(74 citation statements)

References 23 publications

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“…The mixing indices for three different frequencies (1, 3 and 5 Hz) were investigated when the applied negative pressure was set at -80 kPa. Experimental data showed that the samples were completely mixed within 6 s when the driving frequency was 3 Hz (r = 94.5%) (Weng et al 2011). Figure 2 shows the relationship between the flowpumping rate and the applied air pressure with different diameters of the sample transportation units.…”

Section: Characterization Of the Microfluidic Devicesmentioning

confidence: 97%

“…The working principle of the suction-type, pneumatic microfluidic control module for liquid delivery and mixing can be referenced in our previous work (Weng et al 2011). Detail information for the chip fabrication can be found in our previous work (Weng et al 2011).…”

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 98%

“…With this approach, two types of fluidic samples were rapidly mixed with a high mixing efficiency within a short period of time. All the microfluidic elements were driven by regulating electromagnetic valves (EMVs) using digital signals provided by an integrated control circuit with the incorporation of an external vacuum pump (Weng et al 2011). …”

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 99%

“…Detail information for the chip fabrication can be found in our previous work (Weng et al 2011). Briefly, the transport of fluidic samples was enabled when the polydimethylsiloxane (PDMS) membranes of the central mixing/transportation units were deflected upwards sequentially by negative pressure in the air chambers, generated by an external vacuum pump, such that the fluidic sample was drawn into the fluidic reservoirs underneath the central PDMS membrane.…”

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 99%

See 3 more Smart Citations

Integrated microfluidic system for the identification and multiple subtyping of influenza viruses by using a molecular diagnostic approach

Wang

Lien

Hung

et al. 2012

Microfluid Nanofluid

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This study presents an integrated microfluidic system capable of rapid diagnosis of influenza infection and subtyping of influenza viruses. The viral RNA extraction module and reverse-transcription polymerase chain reaction module were integrated with an external optical detection module into the microsystem. Magnetic beads conjugated with specific nucleotide probes were first used to capture target RNA from influenza viral particles after thermolysis/hybridization processes. The hybridized target RNA was purified and collected by an external magnetic field. Finally, the extracted RNA was amplified by one-step RT-PCR products and detected by optical detection module with TaqMan fluorescence system. Moreover, the LOD was experimentally found to be 10 2 copies for influenza A H1/H3 and influenza B viruses. Experimental results also showed that different subtypes of influenza viruses were diagnosed by using multiplex RT-PCR process and automatically completed within 110 min in the developed microfluidic system.

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Section: Characterization Of the Microfluidic Devicesmentioning

confidence: 97%

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 98%

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 99%

Section: Working Principle Of the Diagnostic Assaymentioning

confidence: 99%

See 2 more Smart Citations

Integrated microfluidic system for the identification and multiple subtyping of influenza viruses by using a molecular diagnostic approach

Wang

Lien

Hung

et al. 2012

Microfluid Nanofluid

Self Cite

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show abstract

“…Active fluidic actuation, such as pressure-driven, [4][5][6][7] centrifugal-based, 8 electrokinetic, 9,10 and acoustic 11 pumping, could finely control the liquid flow rate. However, they require costly and burdensome external equipment to build up a functional circuit.…”

Section: Introductionmentioning

confidence: 99%

Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping

Liu

Yang

et al. 2015

Biomicrofluidics

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To sequentially handle fluids is of great significance in quantitative biology, analytical chemistry, and bioassays. However, the technological options are limited when building such microfluidic sequential processing systems, and one of the encountered challenges is the need for reliable, efficient, and mass-production available microfluidic pumping methods. Herein, we present a bubble-free and pumping-control unified liquid handling method that is compatible with large-scale manufacture, termed multilayer microfluidic sample isolated pumping (mlSIP). The core part of the mlSIP is the selective permeable membrane that isolates the fluidic layer from the pneumatic layer. The air diffusion from the fluidic channel network into the degassing pneumatic channel network leads to fluidic channel pressure variation, which further results in consistent bubble-free liquid pumping into the channels and the dead-end chambers. We characterize the mlSIP by comparing the fluidic actuation processes with different parameters and a flow rate range of 0.013 ll/s to 0.097 ll/s is observed in the experiments. As the proof of concept, we demonstrate an automatic sequential fluid handling system aiming at digital assays and immunoassays, which further proves the unified pumping-control and suggests that the mlSIP is suitable for functional microfluidic assays with minimal operations. We believe that the mlSIP technology and demonstrated automatic sequential fluid handling system would enrich the microfluidic toolbox and benefit further inventions. V C 2015 AIP Publishing LLC.

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Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

Gopinathan

Chiang

Bandaru

et al. 2020

Adv Healthcare Materials

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The search of alternative approaches to epithelial cell adhesion molecule (EpCAM), for the isolation of circulating tumor cells (CTC), is on the rise. This work attempts at evaluating the feasibility of using a new glycosaminoglycan, SCH45, as a probe to isolate CTCs from the peripheral blood of 65 advanced/metastatic cholangiocarcinoma (CCA) patients. The positive enrichment of CTCs from 1 mL of blood using SCH45‐bound magnetic beads and subsequent staining on an integrated microfluidic platform is demonstrated. Results detailing CTC concentrations averaging ≥1 CTCs mL−1 of blood are shown, and a conventional protein biomarker, EpCAM, has been used to corroborate the finding that 100% of the patients possess CTCs in their blood. Studies detailing the use of CTCs in the prognostic monitoring and treatment effectiveness of advanced/metastatic CCA are scarce, and the isolation of CTCs from all CCA patients tested has not been reported yet. A strong correlation between CTC counts and disease progression at the time of and/or in advance of radiographic imaging in patients receiving chemotherapy is also reported. This study is one of its kind with the new probe and reduced sample volume and has potential for use in CCA diagnosis and prognosis in the near future.

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A suction-type, pneumatic microfluidic device for liquid transport and mixing

Cited by 73 publications

References 23 publications

Integrated microfluidic system for the identification and multiple subtyping of influenza viruses by using a molecular diagnostic approach

Integrated microfluidic system for the identification and multiple subtyping of influenza viruses by using a molecular diagnostic approach

Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

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