A subunit vaccine based on biodegradable microspheres carrying rHsp65 protein and KLK protects BALB/c mice against tuberculosis infection

Santos, Sónia A.P.; Zárate-Bladés, Carlos R.; Galetti, Fábio Cícero de Sá; Brandão, I. T.; Masson, Ana Paula; Soares, Edson García; Araújo, Ana Paula Ulian de; Silva, Célio Lopes

doi:10.4161/hv.6.12.13350

Cited by 15 publications

(10 citation statements)

References 39 publications

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“…For example, it has been shown that microencapsulation acts in an adjuvant capacity via increased uptake by antigen-presenting cells e APC [32]. The diameter of the MS produced in this work (1e10 mm) was adequate to allow their uptake by professional APC, as shown in other studies [33,34].…”

Section: Discussionmentioning

confidence: 72%

“…Doses of 1e5 mg of MS are used in various oral immunization protocols in humans [41]. A dose of 5 mg/mouse of MS carrying a recombinant heat-shock protein 65 (rHsp65) from Mycobacterium leprae induces a good Ab response in mice [34] and, in the present study, the same dose of MS covalently linked with Ag also generated the maximum response of anti-P. dicentrarchi Ab in mice.…”

Section: Discussionmentioning

confidence: 90%

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A vaccine based on biodegradable microspheres induces protective immunity against scuticociliatosis without producing side effects in turbot

León-Rodríguez

Luzardo-Álvarez

Blanco-Méndez

et al. 2012

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 90%

A vaccine based on biodegradable microspheres induces protective immunity against scuticociliatosis without producing side effects in turbot

León-Rodríguez

Luzardo-Álvarez

Blanco-Méndez

et al. 2012

Fish & Shellfish Immunology

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“…The transfected E. coli was cultured in LB containing ampicillin (100 µ g/ µ l) and the bacterial growth was monitored by spectrophotometry at 600 nm. When the optic density reached a value of 0.6, the culture was induced with isopropylthiogalactoside 0.1 M (Gibco BRL, Gaithersburg, MD, USA) and incubated at 30°C under agitation for 4 h. Details of rhsp65 production and purification can be found at dos Santos et al, 2010 [36]. …”

Section: Methodsmentioning

confidence: 99%

pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity

Zorzella-Pezavento

Chiuso-Minicucci

França

et al. 2017

Journal of Immunology Research

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Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG35–55) emulsified in Complete Freund's Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. MOG35–55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction.

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“…A biodegradable microparticle (BMP) system, which provides sustained release of antigen and adjuvant properties, is capable of meeting these challenges. Several recent studies have demonstrated the utility of this general vaccine approach in vertebrate models [36-40]. Microparticle size is an important determinant for cell uptake [41, 42] and may also influence the antigen release rate [43].…”

Section: Introductionmentioning

confidence: 99%

Single-Dose Microparticle Delivery of a Malaria Transmission- Blocking Vaccine Elicits a Long-Lasting Functional Antibody Response

Dinglasan¹,

Armistead²,

Nyland³

et al. 2013

CMM

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Malaria sexual stage and mosquito transmission-blocking vaccines (SSM-TBV) have recently gained prominence as a necessary tool for malaria eradication. SSM-TBVs are unique in that, with the exception of parasite gametocyte antigens, they primarily target parasite or mosquito midgut surface antigens expressed only inside the mosquito. As such, the primary perceived limitation of SSM-TBVs is that the absence of natural boosting following immunization will limit its efficacy, since the antigens are never presented to the human immune system. An ideal, safe SSM-TBV formulation must overcome this limitation. We provide a focused evaluation of relevant nano-/microparticle technologies that can be applied toward the development of leading SSM-TBV candidates, and data from a proof-of-concept study demonstrating that a single inoculation and controlled release of antigen in mice, can elicit long-lasting protective antibody titers. We conclude by identifying the remaining critical gaps in knowledge and opportunities for moving SSM-TBVs to the field.

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A subunit vaccine based on biodegradable microspheres carrying rHsp65 protein and KLK protects BALB/c mice against tuberculosis infection

Cited by 15 publications

References 39 publications

A vaccine based on biodegradable microspheres induces protective immunity against scuticociliatosis without producing side effects in turbot

A vaccine based on biodegradable microspheres induces protective immunity against scuticociliatosis without producing side effects in turbot

pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity

Single-Dose Microparticle Delivery of a Malaria Transmission- Blocking Vaccine Elicits a Long-Lasting Functional Antibody Response

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