A subtle relationship between substrate stiffness and collective migration of cell clusters

Balcıoğlu, Hayri E.; Balasubramaniam, Lakshmi; Stirbat, Tomita Vasilica; Doss, Bryant L.; Fardin, Marc-Antoine; Mège, René-Marc; Ladoux, Benoît

doi:10.1039/c9sm01893j

Cited by 29 publications

(26 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike on protein-coated surfaces 17 , keratinocytes were not able to form cohesive monolayers on the softer (< 11 kPa) or stiffer (> 90 kPa) nanopatterned surfaces, regardless of spacing. When comparing stiffness alone, higher substrate rigidity induced higher migration speed, as previously reported by others 30,31 (Fig. 4A).…”

Section: Resultssupporting

confidence: 88%

“…While we do observe faster collective migration with increasing substrate stiffness, as has been previously reported (Fig. 4A) 15,30,31 , α5β1 inter-receptor spacing outweighs stiffness effects alone. This could be attributed to integrin α5β1’s role in regulating traction force generation but not rigidity sensing.…”

Section: Discussionsupporting

confidence: 87%

“…4). This was particularly interesting because higher substrate rigidity is generally known to induce greater cellular traction forces, resulting in faster epithelial sheet migration 15,30,31 .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity

Russo

Young

Wegner

et al. 2021

Preprint

View full text Add to dashboard Cite

Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective cell migration. In wound healing, basal keratinocytes collectively migrate on a fibronectin-rich provisional basement membrane to re-epithelialize the injured skin. Among other receptors, the fibronectin receptor integrin α5β1 plays a pivotal role in this process. Using a highly specific integrin α5β1 peptidomimetic combined with nanopatterned hydrogels, we show that keratinocyte sheets regulate their migration ability at an optimal integrin α5β1 nanospacing. This efficiency relies on the effective propagation of stresses within the cell monolayer independent of substrate stiffness. For the first time, this work highlights the importance of extracellular matrix receptor nanoscale organization required for efficient tissue regeneration.

show abstract

Section: Resultssupporting

confidence: 88%

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity

Russo

Young

Wegner

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“… 2018 ; Balcioglu et al. 2020 ). A simple way of testing the impact of stiffness on cellular behavior in tissue culture studies is to change the collagen concentration of the tested gels (Alcaraz et al.…”

Section: Resultsmentioning

confidence: 99%

“…It is well known that interactions between cells and their environment crucially depend on the stiffness of the environment. This holds in particular for the proliferation, survival, migration, and differentiation of cells (Wang et al 2012;Nguyen et al 2018;Balcioglu et al 2020). A simple way of testing the impact of stiffness on cellular behavior in tissue culture studies is to change the collagen concentration of the tested gels (Alcaraz et al 2011;Miroshnikova et al 2011;Hall et al 2016;Joshi et al 2018).…”

Section: Variation Of Collagen Concentrationmentioning

confidence: 99%

A computational framework for modeling cell–matrix interactions in soft biological tissues

Eichinger

Grill

Kermani

et al. 2021

Biomech Model Mechanobiol

View full text Add to dashboard Cite

Living soft tissues appear to promote the development and maintenance of a preferred mechanical state within a defined tolerance around a so-called set point. This phenomenon is often referred to as mechanical homeostasis. In contradiction to the prominent role of mechanical homeostasis in various (patho)physiological processes, its underlying micromechanical mechanisms acting on the level of individual cells and fibers remain poorly understood, especially how these mechanisms on the microscale lead to what we macroscopically call mechanical homeostasis. Here, we present a novel computational framework based on the finite element method that is constructed bottom up, that is, it models key mechanobiological mechanisms such as actin cytoskeleton contraction and molecular clutch behavior of individual cells interacting with a reconstructed three-dimensional extracellular fiber matrix. The framework reproduces many experimental observations regarding mechanical homeostasis on short time scales (hours), in which the deposition and degradation of extracellular matrix can largely be neglected. This model can serve as a systematic tool for future in silico studies of the origin of the numerous still unexplained experimental observations about mechanical homeostasis.

show abstract

Cell Shape and Forces in Elastic and Structured Environments: From Single Cells to Organoids

Link

Weißenbruch

Tanaka

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

With the advent of mechanobiology, cell shape and forces have emerged as essential elements of cell behavior and fate, in addition to biochemical factors such as growth factors. Cell shape and forces are intrinsically linked to the physical properties of the environment. Extracellular stiffness guides migration of single cells and collectives as well as differentiation and developmental processes. In confined environments, cell division patterns are altered, cell death or extrusion might be initiated, and other modes of cell migration become possible. Tools from materials science such as adhesive micropatterning of soft elastic substrates or direct laser writing of 3D scaffolds have been established to control and quantify cell shape and forces in structured environments. Herein, a review is given on recent experimental and modeling advances in this field, which currently moves from single cells to cell collectives and tissue. A very exciting avenue is the combination of organoids with structured environments, because this will allow one to achieve organotypic function in a controlled setting well suited for long‐term and high‐throughput culture.

show abstract

A subtle relationship between substrate stiffness and collective migration of cell clusters

Abstract: The physical cues from the extracellular environment mediates cell signaling spatially and temporally.

Cited by 29 publications

References 87 publications

Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity

Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity

A computational framework for modeling cell–matrix interactions in soft biological tissues

Cell Shape and Forces in Elastic and Structured Environments: From Single Cells to Organoids

Contact Info

Product

Resources

About