A subclass of myosin XI is associated with mitochondria, plastids, and the molecular chaperone subunit TCP‐1α in maize

Wang, Zhengyuan; Pesacreta, Thomas C.

doi:10.1002/cm.10168

Cited by 69 publications

(53 citation statements)

References 73 publications

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“…Roles for class V myosins have also been revealed in the movement of the vacuole, Golgi, and ER elements (38). Strikingly, although no localization within the Golgi apparatus or ER was reported for maize myosin XI in a recent study (39), the same antibodies, generated against a conserved tail region of several isoforms, colocalized with plastids and mitochondria. The reduction in cytoplasmic streaming observed in mya2-1 plants indicates that this myosin class is involved in the motility of higher plant organelles and vesicles and, because of its size-dependent manner, argues for a specific function in the movement of large structures apparently corresponding to the mobile ER of the endoplasm.…”

Section: Involvement In Organelle Movement During Cytoplasmic Streamingmentioning

confidence: 95%

RETRACTED: Arabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport

Holweg

Nick

2004

Proc. Natl. Acad. Sci. U.S.A.

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Myosins are eukaryotic molecular motors moving along actin filaments. Only a small set of myosin classes is present in plants, in which myosins have been found to play a role in cytoplasmic streaming and chloroplast movement. Whereas most studies have been done on green algae, more recent data suggest a role of higher plant myosin at the postcytokinetic cell wall. Here we characterize a loss-of-function mutation for a myosin of plant-specific class XI and demonstrate myosin functions during plant development in Arabidopsis. T-DNA insertion in MYA2 caused pleiotropic effects, including flower sterility and dwarf growth. Elongation of epidermal cells, such as in hypocotyls and anther filaments, was reduced by up to 50% of normal length. This effect on anther filaments is responsible for flower sterility. In the meristems of root tips, it was evident that cell division was delayed and that cell plates were mislocated. Like zwichel, a kinesin-related mutation causing two-branched trichomes, the mya2 knockout causes branching defects, but here the trichomes remained unbranched. Growth was also impaired in pollen tubes and root hairs, cells that are highly dependent on vesicle transport. A failure in vesicle flow could be directly confirmed, because cytoplasmic streaming of vesicles and, more so, of large endoplasmic reticulum-based organelles was slowed. The defect in vesicle trafficking was accompanied by failures in basipetal auxin transport, measured in stem segments of inflorescences. This result strongly suggests a causal link between auxin-dependent processes and the distribution of vesicles and membrane-bound molecules by plant myosin

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Section: Involvement In Organelle Movement During Cytoplasmic Streamingmentioning

confidence: 95%

RETRACTED: Arabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport

Holweg

Nick

2004

Proc. Natl. Acad. Sci. U.S.A.

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“…Antibodies against a conserved Myosin XI peptide label the plasma membrane and maybe mitochondria . Recently, a subclass of Myosin XI has been shown to be associated with mitochondria and plastids (Wang and Pesacreta, 2004). Obviously, polypeptide-specific probes are needed to distinguish individual Myosin XIs in order to reveal their specific intracellular localization patterns.…”

Section: Seventeen Arabidopsis Myosinsmentioning

confidence: 99%

Cytoskeletal Motors in Arabidopsis. Sixty-One Kinesins and Seventeen Myosins

2004

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“…Immunolocalization studies indicated that myosin XIs are associated with various particles in lily (Lilium longiflorum) and tobacco (Nicotiana tabacum) pollen tubes (Yokota et al, 1995), with mitochondria, plastids, and lowdensity membranes in maize (Zea mays) root cells (Liu et al, 2001;Wang and Pesacreta, 2004), and with endoplasmic reticulum (ER) in tobacco BY2 cells . Specific antibodies against MYA2 showed that it is associated with peroxisomes in epidermal and guard cells of Arabidopsis leaves (Hashimoto et al, 2005).…”

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confidence: 99%

A Comparative Study of the Involvement of 17 Arabidopsis Myosin Family Members on the Motility of Golgi and Other Organelles

et al. 2009

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Gene families with multiple members are predicted to have individuals with overlapping functions. We examined all of the Arabidopsis (Arabidopsis thaliana) myosin family members for their involvement in Golgi and other organelle motility. Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ tail or tail domains only were fused to fluorescent markers and coexpressed with a Golgi marker in two different plants. We tracked and calculated Golgi body displacement rate in the presence of all myosin truncations and found that tail fragments of myosins MYA1, MYA2, XI-C, XI-E, XI-I, and XI-K were the best inhibitors of Golgi body movement in the two plants. Tail fragments of myosins XI-B, XI-F, XI-H, and ATM1 had an inhibitory effect on Golgi bodies only in Nicotiana tabacum, while tail fragments of myosins XI-G and ATM2 had a slight effect on Golgi body motility only in Nicotiana benthamiana. The best myosin inhibitors of Golgi body motility were able to arrest mitochondrial movement too. No exclusive colocalization was found between these myosins and Golgi bodies in our system, although the excess of cytosolic signal observed could mask myosin molecules bound to the surface of the organelle. From the preserved actin filaments found in the presence of enhanced green fluorescent protein fusions of truncated myosins and the motility of myosin punctae, we conclude that global arrest of actomyosin-derived cytoplasmic streaming had not occurred. Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells.The Arabidopsis (Arabidopsis thaliana) myosin gene family contains 17 members: myosin group XI, which includes 13 members (myosins XI-

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A subclass of myosin XI is associated with mitochondria, plastids, and the molecular chaperone subunit TCP‐1α in maize

Cited by 69 publications

References 73 publications

RETRACTED: Arabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport

RETRACTED: Arabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport

Cytoskeletal Motors in Arabidopsis. Sixty-One Kinesins and Seventeen Myosins

A Comparative Study of the Involvement of 17 Arabidopsis Myosin Family Members on the Motility of Golgi and Other Organelles

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