A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors

Wang, Zhen; Wang, Li; Zhang, Jia; Li, Yuntao; Zhang, Dongsheng

doi:10.2147/ijn.s16485

Cited by 10 publications

(4 citation statements)

References 11 publications

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“…The main problem associated with the application of such liposomal formulations is insufficient delivery to the target site [ 20 ]. As an interesting approach to drug delivery research, magnetic iron oxides were incorporated into nanoliposomes under the action of a magnetic field could overcome this limitation [ 21 ]. It is documented that the magnetic nanoliposomes that contained doxorubicin or adriamycin were tailored to target cancer cells, and the application of a magnetic field could increase drug concentration in the tumors [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

et al. 2014

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BackgroundArtemisinin is the major sesquiterpene lactones in sweet wormwood (Artemisia annua L.), and its combination with transferrin exhibits versatile anti-cancer activities. Their non-selective targeting for cancer cells, however, limits their application. The aim of this study was to prepare the artemisinin and transferrin-loaded magnetic nanoliposomes in thermosensitive and non-thermosensitive forms and evaluate their antiproliferative activity against MCF-7 and MDA-MB-231 cells for better tumor-targeted therapy.MethodsArtemisinin and transferrin-loaded magnetic nanoliposomes was prepared by extrusion method using various concentrations of lipids. These formulations were characterized for particle size, zeta potential, polydispersity index and shape morphology. The artemisinin and transferrin-loading efficiencies were determined using HPLC. The content of magnetic iron oxide in the nanoliposomes was analysed by spectrophotometry. The in vitro release of artemisinin, transferrin and magnetic iron oxide from vesicles was assessed by keeping of the nanoliposomes at 37°C for 12 h. The in vitro cytotoxicity of prepared nanoliposomes was investigated against MCF-7 and MDA-MB-231 cells using MTT assay.ResultsThe entrapment efficiencies of artemisinin, transferrin and magnetic iron oxide in the non-thermosensitive nanoliposomes were 89.11% ± 0.23, 85.09% ± 0.31 and 78.10% ± 0.24, respectively. Moreover, the thermosensitive formulation showed a suitable condition for thermal drug release at 42°C and exhibited high antiproliferative activity against MCF-7 and MDA-MB-231 cells in the presence of a magnetic field.ConclusionsOur results showed that the thermosensitive artemisinin and transferrin-loaded magnetic nanoliposomes would be an effective choice for tumor-targeted therapy, due to its suitable stability and high effectiveness.

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Section: Discussionmentioning

confidence: 99%

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

et al. 2014

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“…Nanoparticles (NPs) have very specific chemical and physical characteristics in relation to their size, shape and high proportion of surface to volume. These characteristics have made them appropriate for use in multiple medical and biological applications, such as contrast media for magnetic resonance imaging (MRI) ( 1 ), immunoassays ( 2 ), hyperthermia ( 3 ), tissue repair ( 4 ), cellular therapy ( 4 ) and drug delivery ( 5 ). Nanoparticles, consisting of elements such as iron, nickel and cobalt, which exhibit magnetic properties, are called ‘‘magnetic NPs’’.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Iron Oxide Nanoparticles Toxicity on Liver Cells of BALB/c Rats

Parivar

Fard

Bayat

et al. 2016

Iran Red Crescent Med J

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BackgroundBecause of their unique magnetic properties, Fe3O4 nanoparticles (Fe3O4-NPs) have extensive applications in various biomedical aspects. Investigation of the possible adverse aspects of these particles has lagged far behind their fast growing application.ObjectivesThe current study aimed to evaluate the toxicity of Fe3O4-NPs in the liver of mice.Materials and MethodsIn the present clinical trial, 90 BALB/c mice were randomly divided in 15 groups. Five control groups were fed by usual water and food. Five placebo groups were gavaged with physiological serum in doses of 25, 50, 75, 150, and 300 micrograms per gram of body weight (μg/gr). Five experimental groups were gavaged with Fe3O4-NPs, in doses of 25, 50, 75, 150, and 300 μg/gr. This pattern was repeated every other day, for 3 days. Then, the levels of liver enzymes [alanine transaminase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP)] were compared between these groups. The histological alterations of livers were examined, as well. For statistical analysis, Kruskal-Wallis and Mann-Whitney, with type I Bonferroni correction, as post-hoc, have been used.ResultsThe administration of 150 and 300 μg/gr doses of Fe3O4-NPs were associated with significant elevation in liver enzymes, compared to controls (P < 0.0001). Furthermore, the histopathological effects were observed in the liver tissue of these groups. However, in groups treated with lower doses of Fe3O4-NPs, no significant adverse effect was observed.ConclusionsBased on our results, the administration of Fe3O4-NPs causes dose dependent adverse effects on liver.

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“…[ 20 21 ] However, the main problem associated with the application of such artemisinin formulations is insufficient delivery to the target site. [ 22 ]…”

Section: Discussionmentioning

confidence: 99%

Experimental treatment of breast cancer-bearing BALB/c mice by artemisinin and transferrin-loaded magnetic nanoliposomes

Ghorbanihaghjo¹,

Faezizadeh²,

Mesbah-Namin³

et al. 2015

Phcog Mag

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Background:The combination of artemisinin and transferrin exhibits versatile anticancer activities. In previous, we successfully prepared artemisinin and transferrin-loaded magnetic nanoliposomes and evaluated their anti-proliferative activity against MCF-7 and MDA-MB-231 cell lines in vitro. In this study, we investigate the in vivo anti-breast cancer activity of artemisinin and transferrin-loaded magnetic nanoliposome against breast transplanted tumors in BALB/c mice model.Materials and Methods:Artemisinin and transferrin-loaded magnetic nanoliposomes were prepared and characterized for some physiochemical properties. Pieces of tumor tissue from the breast cancer-bearing BALB/c mice were transplanted subcutaneously to the syngeneic female BALB/c mice. In the presence of the external magnet that placed at the breast tumor site, the tissue distribution and tumor-suppressing effects of prepared nanoliposomes on tumor growth was evaluated.Results:The prepared nanoliposomes have fine spherical shape, rough surface, nano-sized diameter and magnetic properties. At 2 h after treatment, the intravenous administration of artemisinin and transferrin-loaded magnetic nanoliposomes followed using the magnetic field approximately produced 10- and 5.5-fold higher levels of artemisinin and transferrin in the tumors, respectively, compared with free artemisinin and transferrin. Moreover, in the presence of an external magnetic field, the prepared nanoliposomes could significantly induce apoptosis in the mice breast cancer cells as well as could reduce tumor volume in tumorized mice at 15 days after treatment.Conclusion:The data suggested that the artemisinin and transferrin-loaded magnetic nanoliposomes would be a good choice for the breast tumor-targeted therapy, due to its high targeting efficiency.

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A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors

Abstract: Purpose: To explore the preparation and characterization of a novel nanosized magnetic liposome containing the PEI-As 2 O 3 /Mn 0.5 Zn 0.5 Fe 2 O 4 complex. Methods: Mn 0.5 Zn 0.5 Fe 2 O 4 and As … Show more

Cited by 10 publications

References 11 publications

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

Evaluation of Iron Oxide Nanoparticles Toxicity on Liver Cells of BALB/c Rats

Experimental treatment of breast cancer-bearing BALB/c mice by artemisinin and transferrin-loaded magnetic nanoliposomes

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