A Study on the Level of Oxidative Stress and Inflammatory Markers in Type 2 Diabetes Mellitus Patients with Different Treatment Modalities

Palem, Siva Prasad; Abraham, Philips

doi:10.7860/jcdr/2015/14218.6449

Cited by 14 publications

(12 citation statements)

References 23 publications

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“…Alteration in insulin resistance predisposes subjects with GDM to occurrence of inflammation which leads to increased levels of inflammatory markers like high sensitive C-reactive protein (hs-CRP) [6]. The exact mechanisms which contribute to increased oxidative stress in hyperglycemia, may include increased non-enzymatic glycosylation and auto-oxidative glycosylation along with the decreased antioxidant defense potential [7].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes

Badehnoosh

Karamali

Zarrati

et al. 2017

The Journal of Maternal-Fetal & Neonatal Medicine

120

143

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes

Badehnoosh

Karamali

Zarrati

et al. 2017

The Journal of Maternal-Fetal & Neonatal Medicine

120

143

View full text Add to dashboard Cite

show abstract

“…MDA, a secondary product of lipid peroxidation, is a common index used to evaluate excess oxidative stress (7,8,25). Excessive quantities of MDA in serum and tissues lead to the development of diabetic vascular complications (14,15,31). In the present study, DHK-medicated serum led to a reduction in the level of MDA (Fig.…”

Section: Discussionsupporting

confidence: 55%

“…The antioxidant status of diabetic patients is crucial in preventing oxidative stress and the process of vascular complications. Antioxidative enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), inhibit the generation of ROS (14)(15)(16)(17), which prevents against endothelial cell injury. Therefore, the correlations among hyperglycemia, redox imbalance and oxidative stress constitute the main pathological mechanism underlying diabetic vascular complications (2).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory effects of DHK-medicated serum on high glucose-induced injury in endothelial cells

Zhang

Chen

Wang

et al. 2017

Molecular Medicine Reports

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It has been shown that oxidative damage and inflammation caused by hyperglycemia in endothelial cells are key factors triggering diabetic vascular complications. The aim of the present study was to investigate the antioxidant and anti‑inflammatory effects of Danhong Huayu Koufuye (DHK)‑medicated serum on high glucose (HG)‑induced injury in endothelial cells, and examine its underlying mechanisms. EA. hy926 cells were treated with normal glucose, HG, or HG with DHK‑medicated serum. Cell viability was assessed using the MTT method. Apoptosis was detected using flow cytometry. Intracellular reactive oxygen species (ROS) levels were measured using the 2',7'‑dichlorodihydrofluorescein method. Cell culture supernatant was collected for detecting the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), and the levels of malondialdehyde (MDA). The protein expression levels of intercellular adhesion molecule‑1 (ICAM‑1), nuclear factor‑κB (NF‑κB), hypoxia‑inducible factor‑1α (HIF‑1α) and vascular endothelial growth factor (VEGF) were determined using western blot analysis. The results revealed that DHK‑medicated serum accelerated the proliferation and inhibited the apoptosis of cells treated with HG (P<0.01) in a dose‑dependent manner. Compared with the HG group, the high levels of ROS and MDA were significantly reduced by DHK‑medicated serum (P<0.01). A 10% concentration of DHK‑medicated serum increased the activities of SOD and GPx by 59.4 and 95.5%, respectively. The high protein expression levels of ICAM‑1, NF‑κB, VEGF and HIF‑1α were significantly ameliorated by DHK‑medicated serum (P<0.01, vs. HG group). These findings indicated that DHK‑medicated serum protected EA. hy926 cells from HG‑induced injury and apoptosis through antioxidation and anti‑inflammatory effects.

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“…It has been well-reported that the oxidative stress was increased in the patients with diabetes and diabetic animals, reflected by enhanced oxidative stress biomarkers in the bloods and tissues, such as malondialdehyde, 3-nitrotyrosine, 4-hydroxynonenal and reactive oxygen species [29,30]. The oxidative stress in the patients with T2DM may play a crucial role in the development of diabetic complications [23,31].…”

Section: Discussionmentioning

confidence: 99%

Genetic variants of nuclear factor erythroid‐derived 2‐like 2 associated with the complications in Han descents with type 2 diabetes mellitus of Northeast China

Sun

Chang

et al. 2016

J Cellular Molecular Medi

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The transcription factor nuclear factor erythroid 2‐like 2 (NFE2L2) is essential for preventing type 2 diabetes mellitus (T2DM)‐induced complications in animal models. This case and control study assessed genetic variants of NFE2L2 for associations with T2DM and its complications in Han Chinese volunteers. T2DM patients with (n = 214) or without (n = 236) complications, or healthy controls (n = 359), were genotyped for six NFE2L2 single nucleotide polymorphisms (SNPs: rs2364723, rs13001694, rs10497511, rs1806649, rs1962142 and rs6726395) with TaqMan Pre‐Designed SNP Genotyping and Sequence System. Serum levels of heme oxygenase‐1 (HMOX1) were determined through enzyme‐linked immunosorbent assay. Informative data were obtained for 341 cases and 266 controls. Between T2DM patients and controls, the genotypic and allelic frequencies and haplotypes of the SNPs were similar. However, there was a significant difference in genotypic and allelic frequencies of rs2364723, rs10497511, rs1962142 and rs6726395 between T2DM patients with and without complications, including peripheral neuropathy, nephropathy, retinopathy, foot ulcers and microangiopathy. Furthermore, HMOX1 levels were significantly higher in T2DM patients with complications than in controls. Multiple logistic regression analysis, however, showed that only rs2364723 significantly reduced levels of serum HMOX1 in T2DM patients for the GG genotype carriers compared with participants with CG+CC genotype. The data suggest that although NFE2L2 rs2364723, rs10497511, rs1962142 and rs6726395 were not associated with T2DM risk, they were significantly associated with complications of T2DM. In addition, only for rs2364723 higher serum HMOX1 levels were found in the T2DM patients with CG+CC than those with GG genotype.

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A Study on the Level of Oxidative Stress and Inflammatory Markers in Type 2 Diabetes Mellitus Patients with Different Treatment Modalities

Cited by 14 publications

References 23 publications

RETRACTED ARTICLE: The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes

RETRACTED ARTICLE: The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes

Antioxidant and anti-inflammatory effects of DHK-medicated serum on high glucose-induced injury in endothelial cells

Genetic variants of nuclear factor erythroid‐derived 2‐like 2 associated with the complications in Han descents with type 2 diabetes mellitus of Northeast China

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