A study on the antioxidant and antimicrobial activities in the chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran

Abstract

Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

“…All samples showed substantial concentrations of total phenolics, with M. hostilis , commercial mixture and the mixture of M. hostilis and B. caapi showing the highest concentrations, and P. harmala the lowest content of total phenolics. Recently, Hadadi et al [ 41 ], developed a study where determined the content of total phenols, using the same method, having also verified the presence of these compounds in extracts of P. harmala .…”

Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan

“…The study of antioxidant activity is useful to provide preliminary data in the development of natural materials to increase the feasibility of using plants as herbal medicines. Antioxidant activity can be determined from the IC50 value by the DPPH method (Hadadi et al, 2020).…”

Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives

“…Sophoraflavanone B ( 48 ) also caused cell wall weakening and consequently membrane damage had occurred, and intracellular constituents leaked from the cell as the proposed mechanism of its antimicrobial activity against methicillin-resistant- Staph. aureus (anti-MRSA) (Farhadi et al 2019 ; Hadadi et al 2020 ). An antibacterial synergism of compound ( 48 ) with antibiotics, including ampicillin, gentamicin, and oxacillin anti-MRSA, was previously determined (Farhadi et al 2019 ).…”