A Study on the Amendment Scheme of Nutrient Standard Regulations for Infant Formula in Korea

Om, Ae-Son; Lee, Heon Ok; Moon, Ji Hea; Shim, Jae-Young; Kim, In Hye; Won, Sun Im; Rha, Young Ah; Choi, Yun Ju; Lee, Hye Young; Park, Hye Kyung; Kim, Myung Chul

doi:10.3746/jkfn.2007.36.5.569

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…The AOAC recently accredited a test for vitamin B 12 for the infant formula matrix, developed ( Kirchner et al , 2011 ). This test showed that the biggest difference between imported and domestic infant formulas and infant growth formulas was the degree of proteolytic degradation of the milk product ( Om et al , 2007 ). It is unlikely that the difference found by this matrix was due to substances in the water-soluble layer interfering with the analysis, as centrifugation was used to remove precipitated proteins, so only the water-soluble nutrients such as vitamin B 12 were likely to remain.…”

Section: Introductionmentioning

confidence: 99%

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Lee¹,

Shin²,

Park³

et al. 2015

Korean Journal for Food Science of Animal Resources

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The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B12 content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B12 was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B12 detection. The calibration curve showed good linearity with the coefficient of correlation (r2) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B12 recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B12 content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B12. This method could be implemented in laboratories that require time and labor saving.

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Section: Introductionmentioning

confidence: 99%

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Lee¹,

Shin²,

Park³

et al. 2015

Korean Journal for Food Science of Animal Resources

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“…The effects of nutritional contents on the normal growth of infants have been extensively studied. ( Neri-Almeida et al, 2009 ; Om et al, 2007 ; Shapiro AD et al, 1986 ; Vasquez-Garibay et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the European Union (EU), Australia, and New Zealand have specified the standard amounts of five nucleotides, namely, cytidine 5′-monophosphate (CMP), uridine 5′-monophosphate (UMP), adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), and inosine 5′-monophosphate IMP) in food. Furthermore, research interests in infant products are continuously being promoted, and standard amounts of nucleotides are now being added to infant food even in Korea ( Om et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector

Won¹,

Bang²,

Kwak³

et al. 2019

Food Sci Anim Resour

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Nucleotides play important roles in numerous intracellular biochemical processes and are used in infant formulas and other dairy products. However, domestic analytical methods for assessing nucleotide content in products have not yet been established, and therefore, methods for determining nucleotide content are urgently required. A rapid and simple analytical method for determining the content of five types of nucleotides in dairy products was improved using solid phase extraction clean-up and high-performance liquid chromatography with diode array detector. The extraction solvent used in the AOAC method was not well dissolved and was changed to hydrophilic EDTA-Na. In addition, the results obtained using the isocratic elution method and a single wavelength were similar to those obtained using the AOAC method, and the time taken for analysis was shortened from 40 min to 25 min. The process of method validation revealed the following parameters: accuracy (84.69%–102.72%), precision (1.51%–6.82%), linearity (0.999), and limit of detection (cytidine 5′-monophosphate, 0.09 mg/L; uridine 5′-monophosphate, 0.11 mg/L; adenosine 5′-monophosphate, 0.12 mg/L; guanosine 5′-monophosphate, 0.11 mg/L; and inosine 5′-monophosphate, 0.14 mg/L). The method was also used to determine the nucleotide concentration in 25 samples (infant formulas, 1.99–29.39 mg/100 g; and cow milk, 0.28–0.83 mg/100 g). The newly improved method was appropriate for analyzing nucleotides in infant formulas and other dairy products faster when compared to conventional methods.

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Infant formula product regulation

Jiang¹

2014

Human Milk Biochemistry and Infant Formula Manufacturing Technology

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A Study on the Amendment Scheme of Nutrient Standard Regulations for Infant Formula in Korea

Cited by 3 publications

References 5 publications

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector

Infant formula product regulation

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A Study on the Amendment Scheme of Nutrient Standard Regulations for Infant Formula in Korea

Cited by 3 publications

References 5 publications

Analytical Determination of Vitamin B12Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Analytical Determination of Vitamin B12Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector

Infant formula product regulation

Contact Info

Product

Resources

About

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Analytical Determination of Vitamin B₁₂Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)