A Study on Surface, Thermal and Mechanical Properties of Absorbable PLGA Plate

Park, JongSoon; Kang, Soon-Kook

doi:10.14257/ijca.2013.6.6.08

Cited by 12 publications

(9 citation statements)

References 16 publications

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“…The PLGA polymer exhibits an event at 56.9°C assigned to the glass transition temperature (T g ); no melting point is observed because this copolymer is amorphous as described elsewhere (Jose et al, 2009;Juríková et al, 2010;Motta and Duek, 2006;Park and Kang, 2013;Vasita et al, 2010). For the DSC curve of the PLGA/ PAC microparticles, a major decrease in the T g to 35.2°C can be observed, suggesting that the incorporation of PAC into the PLGA matrix influences the intermolecular interactions of the copolymer chain.…”

Section: Resultsmentioning

confidence: 93%

SYNTHESIS, CHARACTERIZATION AND in vitro CYTOTOXICITY OF Acacia mearnsii PROANTHOCYANIDIN LOADED PLGA MICROPARTICLES

Grasel

Behrens

Strassburger

et al. 2019

Braz. J. Chem. Eng.

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One of the highlighted areas in the development of new materials is the generation of micro-and nanoparticles as drug carriers which allow the progress in formulations with the ability to release active agents in a controlled way. The proanthocyanidins (PAC) extracted from the bark of the Black Wattle have stood out for their biological activities. However, most polyflavonoids have some features which limit their application in the pharmaceutical field, such as light fastness, low bioavailability of active agents, and unpleasant taste. In this context, this study aims to present the synthesis and characterization of PAC-loaded lactic-co-glycolic acid (PLGA) microparticles obtained by the multiple emulsion method. The incorporation of PAC into PLGA was successfully achieved with PAC encapsulation efficiency around 73%. Spherical microparticles were obtained with a size distribution in the range of 0.6 to 2.4 μm. The presence of PAC modified the thermal properties of the PLGA matrix. The results of in vitro assays with Vero and T24 lineage celss showed that PLGA/PAC microparticles did not promote any effect on cell proliferation by MTT assay after 24 h. The novel Acacia mearnsii proanthocyanidin-loaded PLGA microparticles have potential for application in biological systems.

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Section: Resultsmentioning

confidence: 93%

SYNTHESIS, CHARACTERIZATION AND in vitro CYTOTOXICITY OF Acacia mearnsii PROANTHOCYANIDIN LOADED PLGA MICROPARTICLES

Grasel

Behrens

Strassburger

et al. 2019

Braz. J. Chem. Eng.

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“…PLGA scaffold and its raw material were characterized with FTIR, DSC and TGA [50, 51]. For SCAP and PDLSCs the Infrared Red (IR) spectra were very similar (S1 Fig).…”

Section: Resultsmentioning

confidence: 99%

“…Poly-lactic co-glycolic acid (PLGA) scaffolds with a lactic acid: glycolic acid ratio of 75:25 was prepared as previously described [50, 51]. Briefly, sugar particles measuring 160–200 μm were dispersed in PLGA solution in chloroform.…”

Section: Methodsmentioning

confidence: 99%

hPL promotes osteogenic differentiation of stem cells in 3D scaffolds

et al. 2019

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Human platelet lysate (hPL) has been considered as the preferred supplement for the xeno-free stem cell culture for many years. However, the biological effect of hPL on the proliferation and differentiation of dental stem cells combined with the use of medical grade synthetic biomaterial is still under investigation. Thus, the optimal scaffold composition, cell type and specific growth conditions, yet need to be formulated. In this study, we aimed to investigate the regenerative potential of dental stem cells seeded on synthetic scaffolds and maintained in osteogenic media supplemented with either hPL or xeno-derived fetal bovine serum (FBS). Two types of dental stem cells were isolated from human impacted third molars and intact teeth; stem cells of apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs). Cells were expanded in cell culture media supplemented with either hPL or FBS. Consequently, proliferative capacity, immunophenotypic characteristics and multilineage differentiation potential of the derived cells were evaluated on monolayer culture (2D) and on synthetic scaffolds fabricated from poly ’lactic-co-glycolic’ acid (PLGA) (3D). The functionality of the induced cells was examined by measuring the concentration of osteogenic markers ALP, OCN and OPN at different time points. Our results indicate that the isolated dental stem cells showed similar mesenchymal characteristics when cultured on hPL or FBS-containing culture media. Scanning electron microscopy (SEM) and H&E staining revealed the proper adherence of the derived cells on the 3D scaffold cultures. Moreover, the increase in the concentration of osteogenic markers proved that hPL was able to produce functional osteoblasts in both culture conditions (2D and 3D), in a way similar to FBS culture. These results reveal that hPL provides a suitable substitute to the animal-derived serum, for the growth and functionality of both SCAP and PDLSCs. Thus the use of hPL, in combination with PLGA scaffolds, can be useful in future clinical trials for dental regeneration.

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“…4B). Since no weight loss was seen in thermogravimetric curves in this region, the event can be assigned to a typical enthalpic relaxation superimposed on the glass transition (T g ) characteristic of PLGA materials, [48][49][50] which is evidence of amorphicity. The glass transition temperatures were clearly determined after removing the previous thermal history by thermal treatment of the samples ( Fig.…”

Section: Thermal Characterizationmentioning

confidence: 93%