A Study on Multidrug-Resistant <i>Escherichia coli</i> Clinical Isolates from Different Hospitals in Greater Cairo

Elshimy, Rana; Zedan, Hamdallah; Elmorsy, T. H.; Khattab, Rania Abdelmonem

doi:10.1089/mdr.2020.0521

Cited by 6 publications

(13 citation statements)

References 41 publications

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“…54,55 Treatment of their infections is challenged by the alarming increase in resistance to most of the commonly used antibiotics. 56 Preliminary antimicrobial activities of the different organs of P. obtusa were previously reported; essential oil, chloroform, and ethyl acetate fractions of P. obtusa flowers were active against gram-positive bacteria. 23,57 Moreover, the petroleum ether, ethyl acetate, and isobutanol fractions of P. obtusa leaves were reported to demonstrate antimicrobial activities against different microbial species.…”

Section: Discussionmentioning

confidence: 98%

“…Also, the Shiga toxin-producing E. coli O157:H7 (STEC) is the most common foodborne pathogen and is of big concern to global food safety and public health, , primarily for persons at high risk such as newborns, infants, and immunocompromised individuals in the ICU. , Treatment of their infections is challenged by the alarming increase in resistance to most of the commonly used antibiotics …”

Section: Discussionmentioning

confidence: 99%

“… 54 , 55 Treatment of their infections is challenged by the alarming increase in resistance to most of the commonly used antibiotics. 56 …”

Section: Discussionmentioning

confidence: 99%

“…coli O157:H7, methicillin-resistant S. aureus previously phenotypically and genotypically identified, multidrug-resistant B. cereus, and multidrug-resistant K.…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial Strains and Growth Conditions. The bacterial strains used in the biological assay were multidrugresistant Shiga toxin-producing (STEC) E. coli O157:H7, methicillin-resistant S. aureus previously phenotypically and genotypically identified, 56 multidrug-resistant B. cereus, and multidrug-resistant K. pneumoniae isolated from the intensive care unit of a tertiary care hospital in Cairo. Agarose gel photo documentation of conventional PCR on genetic material extracted from MRSA isolate as a molecular typing for detection of mecA gene using forward primer: GTA GAA ATG ACT GAA CGT CCG ATA A and reverse primer: CCA ATT CCA CAT TGT TTC GGT CTA A were done (Figure S3).…”

Section: Glochiflavanoside B (M3)mentioning

confidence: 99%

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Bioassay-Guided Isolation of Antimicrobial Components and LC/QToF Profile of Plumeria obtusa: Potential for the Treatment of Antimicrobial Resistance

et al. 2023

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The methanolic fraction (M-F) of the total extract (TE) of Plumeria obtusa L. aerial parts showed promising antibacterial effects against the MDR (multidrug-resistant) gram-negative pathogens Klebsiella pneumoniae and Escherichia coli O157:H7 [Shiga toxin-producing E. coli (STEC)]. In addition, M-F had a synergistic effect (in combination with vancomycin) against the MDR gram-positive strains MRSA (methicillin-resistant Staphylococcus aureus) and Bacillus cereus. After treating the K. pneumoniae- and STEC-infected mice with M-F (25 mg/kg, i.p.), the level of IgM and TNF-α was decreased and the severity of pathological lesions were reduced better than that observed after administration of gentamycin (33 mg/kg, i.p.). Thirty-seven compounds including 10 plumeria-type iridoids and 18 phenolics, 7 quinoline derivatives, 1 amino acid, and 1 fatty acid were identified in TE using LC/ESI-QToF. Furthermore, five compounds; kaempferol 3-O-rutinoside (M1), quercetin 3-O-rutinoside (M2), glochiflavanoside B (M3), plumieride (M4), and 13-O-caffeoylplumieride (M5) were isolated from M-F. M5 was active against K. pneumoniae (MIC of 64 μg/mL) and STEC (MIC of 32 μg/mL). These findings suggested that M-F and M5 are promising antimicrobial natural products for combating MDR K. pneumoniae and STEC nosocomial infections.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

“… 54 , 55 Treatment of their infections is challenged by the alarming increase in resistance to most of the commonly used antibiotics. 56 …”

Section: Discussionmentioning

confidence: 99%

“…coli O157:H7, methicillin-resistant S. aureus previously phenotypically and genotypically identified, multidrug-resistant B. cereus, and multidrug-resistant K.…”

Section: Methodsmentioning

confidence: 99%

Section: Glochiflavanoside B (M3)mentioning

confidence: 99%

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Bioassay-Guided Isolation of Antimicrobial Components and LC/QToF Profile of Plumeria obtusa: Potential for the Treatment of Antimicrobial Resistance

et al. 2023

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Prevalence and In Vivo Assessment of Virulence in Shiga Toxin-Producing Escherichia coli Clinical Isolates from Greater Cairo Area

Elshimy,

Zedan,

Elmorsy

et al. 2023

Microbial Drug Resistance

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Phenotypic and Genotypic Analysis of Bacterial Pathogens Recovered from Patients Diagnosed with Fever of Unknown Origin in Egypt

Mostafa¹,

Saleh

Khaleel

et al. 2023

Antibiotics

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Fever of unknown origin (FUO) is a medical term describing fever that lasts for at least three weeks without a diagnosis being reached after extensive diagnostic evaluation. Therefore, this study aimed to identify the common pathogens causing FUO in patients admitted to Abbasia Fever Hospital in Egypt from January 2020 to December 2022, their antimicrobial susceptibility profiles, and associated resistance genes. The study also aimed to investigate the burden of multidrug-resistant (MDR) pathogens and the priority pathogens nominated by the World Health Organization (WHO) for posing the greatest threat to human health due to antibiotic resistance. During the study period, about 726 patients were diagnosed with FUO. After extensive investigations, the cause of the FUO was found to be infectious diseases in 479/726 patients (66.0%). Of them, 257 patients had positive bacterial cultures, including 202 Gram-negative isolates that comprised Klebsiella pneumoniae (85/202; 42.1%), Escherichia coli (71/202; 35.1%), Acinetobacter baumannii (26/202; 12.9%), and Pseudomonas aeruginosa (14/202; 6.9%) and 55 Gram-positive isolates, including Staphylococcus aureus (23/55; 41.8%), Streptococcus pneumoniae (7/55; 12.7%), and Enterococcus spp. (25/55; 45.5%). The MDR phenotype was shown by 68.3% and 65.5% of the Gram-negative and Gram-positive isolates, respectively. Carbapenem resistance (CR) was shown by 43.1% of the Gram-negative isolates. Of the 23 S. aureus isolates obtained from research participants, 15 (65.2%) were methicillin-resistant S. aureus (MRSA). A high-level aminoglycoside resistance (HLAR) phenotype was found in 52.0% of the Enterococcus sp. isolates. The PCR screening of resistance genes in the MDR isolates showed that blaOXA−48 was the most prevalent (84%) among the carbapenemase-coding genes, followed by blaVIM (9%) and then blaIMP (12%). The ESBL-coding genes blaTEM, blaCTX-M,aac(6′)-Ib, and blaSHV, were prevalent in 100%, 93.2%, 85,% and 53.4% of the MDR isolates, respectively. This study updates the range of bacteria that cause FUO and emphasizes the burden of multidrug resistance and priority infections in the region. The obtained data is of relevant medical importance for the implementation of evidence-based antimicrobial stewardship programs and tailoring existing empirical treatment guidelines.

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A Study on Multidrug-Resistant Escherichia coli Clinical Isolates from Different Hospitals in Greater Cairo

Cited by 6 publications

References 41 publications

Bioassay-Guided Isolation of Antimicrobial Components and LC/QToF Profile of Plumeria obtusa: Potential for the Treatment of Antimicrobial Resistance

Bioassay-Guided Isolation of Antimicrobial Components and LC/QToF Profile of Plumeria obtusa: Potential for the Treatment of Antimicrobial Resistance

Prevalence and In Vivo Assessment of Virulence in Shiga Toxin-Producing Escherichia coli Clinical Isolates from Greater Cairo Area

Phenotypic and Genotypic Analysis of Bacterial Pathogens Recovered from Patients Diagnosed with Fever of Unknown Origin in Egypt

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