A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation

Takamiya, Masataka; Kumagai, Reiko; Nakayashiki, Nori; Aoki, Yasuhiro

doi:10.1016/j.legalmed.2006.05.002

Cited by 14 publications

(7 citation statements)

References 26 publications

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“…Inflammatory cell dynamics [1] and enzyme activities, such as ATPase, acid phosphatase, and alkaline phosphatase [10] are still applied to wound age estimation. Analyses of cytokine expressions in murine skin and immunohistochemical detection in human skin have also been reported, and their usefulness for dermal wound age estimation has been suggested [9,16,22,31,32].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

et al. 2007

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We performed quantification of IL 2, IL 4, IL 6, IL 8, IL 10, GM-CSF, IFN gamma, and TNF alpha in human dermal wounds for wound age estimation. The proliferation of dermal cells and infiltration of inflammatory cells were also analyzed. Neutrophils and macrophages were detected from 2 h post-injury, and strong infiltrations were seen at 33-49 h. T and B lymphocytes also infiltrated simultaneously from 71 h. Strong proliferation of fibroblasts were shown from 246 h, and thickening of the epidermis from 71 h. IL 10, GM-CSF, IFNgamma, and TNF alpha increased from the early phase of dermal wound healing, IL 6 exclusively in the middle phase, IL 2, IL 4, and IL 8 from the middle phase to the late phase. Among the cytokines analyzed in the present study, IL 6, IL 8, IFNgamma, and TNF alpha were strongly expressed. Results of the present study suggest that multiplex cytokine analysis at the wound site can be useful for wound age estimation. In addition, multiplex data obtained from the same sample with a single method would demonstrate more accurate interactions of cytokines during dermal wound healing. Although the present study was oriented to practical forensic pathology, the data obtained would be informative for various fields of medicine.

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Section: Introductionmentioning

confidence: 99%

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

et al. 2007

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“…In dating such wounds, the dynamics of the astroglia and inflammatory cells (Hausmann et al, 1999) have been employed to achieve lesion dating. Immunohistochemical analyses of human cerebral wounds , 2002Orihara et al, 2001Orihara et al, , 2002Orihara et al, , 2003 and those of cytokine mRNA in the murine brain (Takamiya et al, 2003(Takamiya et al, , 2006 have also been reported, with the assertion that they are also useful for cerebral wound age estimation.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detections of 27 Cytokines during Cerebral Wound Healing by Multiplexed Bead-Based Immunoassay for Wound Age Estimation

Takamiya

Fujita

Saigusa

et al. 2007

Journal of Neurotrauma

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Quantification of 27 cytokines following cerebral wounding was performed for wound age estimation. The cytokines evaluated included interleukin (IL)-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12 p40, IL-12 p70, IL-15, IL-17, IL-18, basic fibroblast growth factor (bFGF), granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), Interferon-gamma (IFN-gamma), keratinocyte derived cytokine (KC), leukemia inhibitory factor (LIF), macrophage-colony stimulating factor (M-CSF), monokine inducible by interferon gamma (MIG), macrophage inflammatory protein (MIP)-1 alpha, MIP 2, platelet-derived growth factor BB (PDGF BB), regulated upon activation, normal T-cell expressed, and secreted (Rantes), tumor necrosis factor-alpha (TNF-alpha), and vascular endothelial growth factor (VEGF). The proliferation of glial cells as well as the infiltration of inflammatory cells were also evaluated. Although astroglia proliferated from 72 hours post-injury, inflammatory cell dynamics were generally steady. Among cytokines analyzed in the present study, IL-1beta, IL-5, IL-6, IL-12 p40, G-CSF, IFN-gamma, KC, LIF, MIP2, and PDGF BB increased during the early phase of cerebral wound healing, and M-CSF increased during the middle phase, while IL-15, IL-18, and MIG increased during the late phase. In contrast, IL-1alpha, IL-10, IL-12 p70, and TNF-alpha were suppressed throughout the cerebral wound healing process. Based on our findings, quantitative cytokine analyses at the cerebral wound site may be a useful tool for wound age estimation. Further, this study suggests that multiplex data gained from the same sample using a single methodology demonstrates highly accurate cytokine interactions during the process of cerebral wound healing.

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“…Multiple approaches and techniques have been used to determine wound vitality and time periods. In addition to proteins [11], transcripts have also been used as markers to analyze the earlier stages of vitality in recent decades [12][13][14]. It would seem to be more appropriate to assess mRNA, by RT-PCR, in early wound age estimations, because mRNA appears earlier than the protein it encodes [15].…”

Section: Discussionmentioning

confidence: 99%

An “up, no change, or down” system: Time-dependent expression of mRNAs in contused skeletal muscle of rats used for wound age estimation

Sun

Zhu

Dong

et al. 2017

Forensic Science International

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The combined use of multiple markers is considered a promising strategy in estimating the age of wounds. We sought to develop an "up, no change, or down" system and to explore how to combine and use various parameters. In total, 78 Sprague Dawley rats were divided randomly into a control group and contusion groups of 4-, 8-, 12-, 16-, 20-, 24-, 28-, 32-, 36-, 40-, 44-, and 48-h post-injury (n=6 per group). A contusion was produced in the right limb of the rats under diethyl ether anesthesia by a drop-ball technique; the animals were sacrificed at certain time points thereafter, using a lethal dose of pentobarbital. Levels of PUM2, TAB2, GJC1, and CHRNA1 mRNAs were detected in contused muscle using real-time PCR. An up, no change, or down system was developed with the relative quantities of the four mRNAs recorded as black, dark gray, or light gray boxes, representing up-, no change, or down-regulation of the gene of interest during wound repair. The four transcripts were combined and used as a marker cluster for color model analysis of each contusion group. Levels of PUM2, TAB2, and GJC1 mRNAs decreased, whereas that of CHRNA1 increased in wound repair (P<0.05). The up, no change, or down system was adequate to distinguish most time groups with the color model. Thus, the proposed up, no change, or down system provide the means to determine the minimal periods of early wounds.

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A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation

Cited by 14 publications

References 26 publications

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

Simultaneous Detections of 27 Cytokines during Cerebral Wound Healing by Multiplexed Bead-Based Immunoassay for Wound Age Estimation

An “up, no change, or down” system: Time-dependent expression of mRNAs in contused skeletal muscle of rats used for wound age estimation

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