A study on Hydrocarbon Degradation by Biosurfactant Producing Bacillus cereus in Oil Contaminated Soil Samples

Abstract

Microorganisms are the important factors in the degradation of the toxic substances in our environment.Petrol and diesel oil is one of the complex mixtures which cannot be easily degraded. The Bacillus cereus was involved in the degradation of oil during which the complex toxic substances were detoxified by the production of biosurfactants. In our study we have identified that the biosurfactant producing Bacillus cereus have a high potential for hydrocarbon degradation. The Bacillus cereus was isolated from hydrocarbon contaminated soil and identified based on morphology and biochemical test according to the Bergey's manual of systematic bacteriology. The maximum hydrocarbon degrading biosurfactant producing Bacillus cereus was obtained by qualitative and quantitative methods. In optimization studies, the best results observed for Bacillus cereus were, Olive oil as the suitable carbon source, Sodium nitrate as the best Nitrogen source and Optimum pH is 7 and Optimum temperature is 37°C. The ability of these isolates to degrade hydrocarbons and survive in the oil contaminated soil is attributed to the development of resistance by mutation on the plasmid. It is also clearly evident that the specific gene was responsible for the production of biosurfactant and the degradation process. According to the results from the present study the Bacillus cereus has high potential for hydrocarbon degradation and can be used especially for Microbial Enhanced Oil Recovery and bioremediation of hydrocarbons in near future.

Acreditation Certificate Acreditation No. 21/E/KPT/2018 Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Ainun Ramadhani Tri Wahyuni ORCID iD https://orcid.org/0000-0002-4071-3406 Fisheries and Marine Science Faculty, Brawijaya University Indonesia Endang Yuli Herawati Fisheries and Marine Science Faculty, Brawijaya University Indonesia Andi Kurniawan ORCID iD Fisheries and Marine Science Faculty, Brawijaya University Indonesia Ab

“…Indigenous bacterial isolates have a yellowish pigmented colony, irregular in shape, with a high edge (lobate), high elevation (raised), and rough and regular internal structures. This result was similar to Janaki et al (2016), mentioned that Bacillus cereus has a high potential for hydrocarbon degradation by producing biosurfactant.…”

Identification and Isolation of Biosurfactant Producing Bacteria from Oil Spill Contaminated Soil of Ennore

“…This demonstrated their ability to create biosurfactants that lowered interfacial and surface tension, resulting in an increase in oil solubility and emulsification 24 . Bacillus subtilis and Bacillus cereus, which were isolated from water and soil, respectively, were discovered to have the properties of biosurfactants as mentioned in this study 25 .…”

Isolation and characterization of fungi and bacteria able to grow on media containing gasoline and diesel fuel

“…The bacterial growth increased with increasing diesel concentration but decreased at 25 % of diesel 34 . B. cereus utilizes the hydrocarbons as a sole carbon source for their growth by degrading the hydrocarbon due to the production of biosurfactant 35 . B. pumilus produces biosurfactants and has shown very high degrada-tion potential for diesel oil and waste engine oil hydrocarbons 36 .…”