A study on biological activity measurements and heterotrophic bacteria in a small freshwater lake

Tiwari, B. K.; Mishra, Ruchi

doi:10.1007/bf00016407

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…However, this substrate has a high background fluorescence which necessitates use of a low substrate concentration which might be in conflict with the need to have the reaction proceed at substrate saturation. Hitherto the most common substrate in freshwater studies has been p-NPP, p-nitrophenylphosphate, where the reaction product p-nitrophenol is detected calorimetrically (Reichardt etal., 1967;Berman, 1969Berman, , 1970Reichardt, 1971Reichardt, , 1973Jones, 1972a,b;Heath & Cooke, 1975;Verstraete et al, 1976;Stevens & Parr, 1977;Wynne, 1977Wynne, , 1981Sayler et al, 1979;Fuhs et al, 1982;Tiwari & Mishra, 1982;Livingstone et al, 1983 ;Livingstone & Witton, 1984;Matavulj et al, 1984;Chrost et al, 1984;Klotz, 1985a,b). The substrate p-NPP offers a reliable determination when working with high phosphatase concentrations as in algal cultures or if long incubation times can be tolerated.…”

Section: Measurements Of Phosphatase Activitymentioning

confidence: 99%

“…z = zooplankton) P = pond Type of phosphatases assayed (AC = acid N = neutral Al = alkaline) Pore-size % of total if filter phosphawas used tase (w) activity (-= not specified) (centr. = centrifugation) Jansson et&, 1981Reichardt, 1971Jansson, 1976Jones, 1972a,b Chrost et al, 1984Pettersson, 1980Francko, 1983Tiwari & Mishra, 1982Berman, 1970Francko, 1984bGunatilaka, 1984HeaJey & Hendzel, 1979Wetzel, 1981Reichardt et al, 1967Stewart & Wetzel, 1982aOverbeck, 1964Berman & Moses, 1972Aaronson & Patni, 1976Antia & Watt, 1964Wyrme, 1981Aaronson, 1971Patni et al, 1977Huber et al, 1983Healey, 1973Healey, 1985Healey & Hendzel, 1979Lin, 1977Kuenzler & Perras, 1965Walther & Fries, 1976Rigler, 1961 Both alkaline and acid phosphatases have been found as external and internal enzymes in algae and bacteria (Siuda, 1984) and both types can be excreted by zooplankton (Wynne & Gophen, 1981). The alkaline phosphatases with external function are by far the most studied type in aquatic ecosystems.…”

Section: Characteristics Of Phosphatasesmentioning

confidence: 99%

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Phosphatases; origin, characteristics and function in lakes

Jansson¹,

Olsson²,

Pettersson³

1988

Hydrobiologia

272

150

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Phosphatases catalyze the liberation of orthophosphate from organic phosphorus compounds. The total phosphatase activity in lake water results from a mixture of phosphatases localized on the cell surfaces of algae and bacteria and from dissolved enzymes supplied by autolysis or .excretion from algae, bacteria and zooplankton. External lake water phosphatases usually have pH optima in the alkaline region. Acid phosphatases generally seem to be active in the internal cell metabolism. The synthesis of external alkaline phosphatases is often repressed at high phosphate concentrations and derepressed at low phosphate concentrations. Phosphatase activity has therefore been used as a phosphorus deficiency indicator in algae and in natural plankton populations. The possibilities for this interpretation of phosphatase activity in lake water are limited, however, and this is discussed. The in situ hydrolysis capacity, i.e. the rate by which orthophosphate is released from natural substrates, is unknown. However, we advocate that this process is important and that the rate of substrate supply, rather than phosphatase activity, limits the enzymatic phosphate regeneration.

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Section: Measurements Of Phosphatase Activitymentioning

confidence: 99%

Section: Characteristics Of Phosphatasesmentioning

confidence: 99%

Phosphatases; origin, characteristics and function in lakes

Jansson¹,

Olsson²,

Pettersson³

1988

Hydrobiologia

272

150

View full text Add to dashboard Cite

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Spatial and seasonal distribution of heterotrophic bacteria in pond water and sediments under different management practices

Jana

1990

Intl Review of Hydrobiology

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Population densities and generation time of heterotrophic bacteria were estimated i n water and sediments of fish ponds used for polyculture, monoculture and traditional systems of fish farming over a period of two and a half years. Spatial differences in microbial densities in the ponds weqe rrlated t o the organic loadings of the farm site and farm management. A number of environmental factors such as nitrogen, dissolved organic carbon, bicarbonate and phosphate of water were found to be responsible for the seasonnl changes of heterotrophic bacteria in these ponds. Content 8 9 0 0 50 0 100 I b l MONO CULTURE-2 J O J A J O J A J O 1978 1979 1980 Fig. 6. Seasonal coiirse of heterotrophic buctcria in the sediments of monoculture-1 (a) a t Kalymi and monoculture-2 (1)) 1Lt Naihati. Sine and cosino waves was fitted to observed mcan annual data of monocultiirc-1 (c) at Kiilycini (r,=0.678; P-=O.O:S) and monoculturc-2 ( d ) a t Xaihati (r,=0.620; P-=0.05). 1978 I L L I J , I~L L I . i . L d O J A J O 1979 1980 J O J A J O J A J O 1978 3979

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