Phosphatases; origin, characteristics and function in lakes

Jansson, Mats; Olsson, Håkan; Pettersson, Kurt

doi:10.1007/bf00024903

Cited by 270 publications

(164 citation statements)

References 87 publications

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“…Different groups of bacteria showed maximum hydrolyzing activity with phosphatase either at alkaline or acidic pH and hence two categories viz. alkaline and acidic phosphatases have been recognized [25]. In this study, phosphatase activity of different isolates showed maximum activity in different alkaline pH condition (Fig.…”

Section: Resultsmentioning

confidence: 57%

Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar

Ethiraj

Ravindran

2011

J. Basic Microbiol.

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Phosphatase Producing Bacteria (PPB) and Inorganic Phosphate Solubilizing Bacteria (IPSB) are important to reef nutrition. These microbes and phosphate concentration play a significant role in the productivity of coral reef ecosystems. A study was conducted in Gulf of Mannar coral reef ecosystem to understand the diversity of these groups of bacteria and their competence in mineralizing the phosphate. The PPB isolates were identified under six genera i.e. Bacillus, Pseudomonas, Micrococcus, Vibrio, Arthrobacter and Brevibacterium. Likewise, the IPSB isolates were also identified that belong to six genera i.e. Bacillus, Arthrobacter, Pseudomonas, Flavobacterium, Flavomonas and Micrococcus. Among the PPB and IPSB strains, Bacillus represented more number of species than others. PPB exhibited maximum activity between pH 8 and 9 and the lowest was at pH 6. Among the phosphatase producers Bacillus cereus (546) showed maximum activity (0.333 mmol/l P) at pH 8. In general, the phosphatase activity in most of the Bacillus species varied with reference to different pH. The species Bacillus megaterium (573) showed highest phosphate solubilizing activity (0.906 mmol/l P) by producing 2-ketogluconic acid. The production of organic acids and phosphatase enzymes by these bacterial groups are responsible for the conversion of insoluble inorganic and organic phosphates into soluble forms which are available for the reef organisms.

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Section: Resultsmentioning

confidence: 57%

Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar

Ethiraj

Ravindran

2011

J. Basic Microbiol.

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“…Phosphatases promote the degradation of complex phosphorous compounds and have an essential function in the nutrient dynamics of lakes in absence of external natural phosphorus input process therein [2]. The ALPs (EC 3.1.3.1) are by far the most important group in the aquatic environment, since the conditions favouring the acid phosphatases are quite uncommon in aquatic environment and the algae and bacteria in lakes are mostly favoured by alkaline environment and generally produce more alkaline than acid phosphatases [3].…”

Section: Introductionmentioning

confidence: 99%

Novel Organophosphate Pesticide Utilizing Alkaline Phosphatase Producing Polyextremophile Bacillus Flexus from Lake Ecosystem of North Gujarat, India

Falguni¹,

Sharma²

2014

IJIRSET

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ABSTRACT:The biodiversity of alkaline phosphatase (ALP) producing microbes from the lake ecosystem of the North Gujarat region was explored. Methyl Green dye and Phenolphthalein diphosphate supplemented MG-PDP medium screened ALP producing microbes as deep green stained cultures. The best ALP producing isolate was put through phenetic and genetic identification on the basis of morphology, biochemistry, physiology and 16S rRNA analysis. This best ALP producer proved to be catalase and oxidase positive, spore forming, noncapsulated, motile, vancomycin sensitive Gram-positive rod which gives optimum ALP production at pH 9.0, 35ºC and 0.5% NaCl concentration. Scanning Electron Micrographs revealed unique pili like bridge between bacterial cells and no flagella. N-BLAST search of the 16S rRNA sequence of the isolate (GenBank accession no JN415115) exhibited maximum similarity (98%) with Bacillus flexus. Organophosphorous Pesticide Acephate was utilized as sole Carbon source accompanied with the release of inorganic phosphate indicating its role in organophosphate pesticide bioremediation.

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“…Phosphatases are enzymes, which promote the degradation of complex phosphorus compounds into orthophosphate and an organic moiety, are thus believed to have an essential function in the nutrient dynamics of most of the ecological niches [11]. Microorganisms with phosphatase activity are able to hydrolyze phosphate from a variety of organic phosphorus compounds [2,3].…”

Section: Introductionmentioning

confidence: 99%

Phosphatase Activity of Non-Hair Forming Cyanobacterium Rivularia and Its Role in Phosphorus Dynamics in Deepwater Rice-Fields

Banerjee¹,

John²

2005

Appl Ecol Env Res

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Abstract. Phosphatase activity of Rivularia strains II and I isolated from a deepwater rice field was studied. When grown under conditions of P-limitation the strains did not form hair and showed great variations in induction time and pattern of enzyme activity in relation to growth. Phosphomonoesterase activity (PMEase) was induced earlier in the Rivularia strain I which was maintained in high phosphate concentration in the medium before the study. Rivularia strain II on the other hand showed induction of PMEase activity after 3-4 days and this strain was maintained in low phosphorus concentrations in the medium before the study. The comparison of K m and V max values early in the growth phase showed that affinity of both PMEase (phosphomonester) and PDEase (phosphodiester) for the substrate was higher in early growth phase rather than in later stages suggesting that there is presence of more than one phosphatase enzymes in these organisms. The observations from the above study have important implications in rice fields where constant fluctuations in nutrients can be observed -especially by phosphorus in its most available form phosphate. Equal enzyme activity in both light and dark for the two strains (non hair-forming) implies that phosphatase activity may not be affected by changes in light regimes in rice fields which do occur due to the plant growth. This can in turn contribute significantly to maintain the growth of cyanobacteria for prolonged periods even under unfavorable conditions of phosphorus depletions. These strains of Rivularia can therefore biotechnologically exploited as biofertilizers (phosphatic fertilizer) when there is phosphorus deficiency. During the unfavorable conditions of phosphorus deficiency, phosphatase activity may play a significant role in providing phosphate for its own growth and for the rice plants.

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Phosphatases; origin, characteristics and function in lakes

Cited by 270 publications

References 87 publications

Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar

Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar

Novel Organophosphate Pesticide Utilizing Alkaline Phosphatase Producing Polyextremophile Bacillus Flexus from Lake Ecosystem of North Gujarat, India

Phosphatase Activity of Non-Hair Forming Cyanobacterium Rivularia and Its Role in Phosphorus Dynamics in Deepwater Rice-Fields

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