A Study of Thyrocalcitonin Secretion by Direct Measurement of<i>in Vivo</i>Secretion Rates in Pigs<sup>1</sup>

Care, A. D.; Cooper, Cary W.; Duncan, Thomas; Orimo, Hajime

doi:10.1210/endo-83-1-161

Cited by 65 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The studies of , - Lee et al ( 1969), and Cooper et al (1971) confirmed the observations of Care et al (1968) ----that the rate of CT secretion was directly related to the degree of hypercalcemia. Moreover, these studies demonstrated that CT was -4 Jt inuously secreted and that the rate of hormone secretion was con under ~irectly proportional control of blood calcium even in the normocalcemic range.…”

Section: Mechanism Of Actionsupporting

confidence: 76%

“…Care et al (1968) examined the relationship between CT secretion rate and plasma calcium concentration in pigs in an endeavor to clarify the control mechanism of CT secretion as related to blood -39-calcium. Pig thyroid glands, which have no embedded parathyroid tissue, were surgically isolated and perfused with blood, the calcium concentration of which varied between 5 to 11 mEq/l.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

“…Although Care et al (1968) had suggested that CT was secreted at normocalcemic ranges and the experimental evidence of Klein and Talmage (1968) and Minkin and Talmage (1968) (1968). The concentration of· CT found in thyroid effluent blood in unstimulated rabbits was 10 mµg/ml while that in the peripheral cir--41-cuiation was 0.14 mµg/ml.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

See 2 more Smart Citations

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Oeltgen¹,

L'Heureux²

1975

Horm Metab Res

View full text Add to dashboard Cite

Mitochondria, nu dei, and microsomes obtained from ovine thyroid slices wh ich had been incubated in Krebs-Ringer bicarbonate buffer containing 4.8, 11.5 or 26.0 mg% concentration of calcium were assayed for calcitonin activity in rats. The microsomal preparatioa was the only particulate fraction to exhibit marked hypocalcemic activity and the hormonal activity of this fraction was enhanced by a 26.0 mg% calcium concentration in the incubation medium. The identity of the hypocalcemic species in the microsomal fraction with a calcitonin preparation 01' known potency was established by polyacrylamide gel eletrophoresis and by isoelectric focusing. The ovine microsomal calcitonin was resolved into two pro tein bands with isoelectric points (pl's) of 3.49 and 3.84. Both of these protein peaks exhibited hypocalcemic activity. The porcine standard calcitonin was resolved into two protein bands with pl's of 3.70 and 3.94.

show abstract

Section: Mechanism Of Actionsupporting

confidence: 76%

Section: Mechanism Of Actionmentioning

confidence: 99%

Section: Mechanism Of Actionmentioning

confidence: 99%

See 1 more Smart Citation

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Oeltgen¹,

L'Heureux²

1975

Horm Metab Res

View full text Add to dashboard Cite

show abstract

“…CT works to lower the serum Ca level by inhibiting the osteoclast activity when the serum Ca level was elevated [6]. In fractured horses, the CT level was elevated significantly before the accidents.…”

Section: Discussionmentioning

confidence: 99%

“…Serum PTH level is controlled by blood Ca level through Ca-sensing receptors [4]. On the other hand, calcitonin (CT) is secreted from para-follicular cells (C cells) of the thyroid to inhibits the elevation of serum Ca when it is high [6,7]. However, serum levels of these calcium-regulating hormones in racehorses have not been investigated very well.…”

mentioning

confidence: 99%

Serum Parathyroid Hormone and Calcitonin Levels in Racehorses with Fracture.

Chiba

Kanematsu

Murakami

et al. 2000

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

. Serum parathyroid hormone (PTH) and calcitonin (CT) levels in fractured racehorses were measured by radioimmunoassay. Racehorses with fracture of large bone such as the radius, third metacarpus, third carpus, digital bone or tibia, showed normal PTH level and elevated CT level in the serum. Serum PTH level was slightly higher in racehorses with sesamoid bone fracture compared to that of healthy racehorses, but not statistically significant. Moreover, serum CT level of racehorses with sesamoid bone fracture was significantly higher than that of healthy racehorses. Racehorses with sesamoid bone fracture and large bone fracture might be in different conditions of calcium regulation.-KEY WORDS: calcitonin, fracture, parathyroid hormone, racehorse, radioimmunoassay.

show abstract

Cosecretion of calcitonin and calcitonin gene-related peptide from cultured rat medullary thyroid C cells

Seitz

Cooper

1989

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

Whether C cells cosecrete calcitonin (CT) and CGRP was examined by exposing cultured rat medullary thyroid carcinoma 6-23 cells for 2 h to high medium Ca and to agents with a potential for affecting Ca-dependent secretion. In every experiment exposure of cells to high medium Ca (2.0-2.5 mM) provoked an increased release of both peptides that was highly correlated (r = 0.73). With other test substances, also, changes in both hormones occurred in parallel. The Ca-channel activator, BAY-K-8644 (10 microM) increased secretion, and this was inhibited by the Ca channel blocker, nitrendipine (10 microM). The Ca2+ ionophore, ionomycin (5 microM), increased release, and the calmodulin-Ca channel inhibitor, phenytoin (100 microM), inhibited Ca-induced release. The active 4 beta isomer of phorbol-12,13-didecanoate (0.1 microM), but not the inactive 4 alpha isomer, increased secretion. The findings suggest that pathways mediating C cell secretion include plasma membrane Ca channels, intracellular [Ca2+], calmodulin, and protein kinase C. The results show that the secretory process in rat C cells involves the release of CGRP as well as CT.

show abstract

A Study of Thyrocalcitonin Secretion by Direct Measurement ofin VivoSecretion Rates in Pigs¹

Cited by 65 publications

References 0 publications

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Serum Parathyroid Hormone and Calcitonin Levels in Racehorses with Fracture.

Cosecretion of calcitonin and calcitonin gene-related peptide from cultured rat medullary thyroid C cells

Contact Info

Product

Resources

About

A Study of Thyrocalcitonin Secretion by Direct Measurement ofin VivoSecretion Rates in Pigs1

Cited by 65 publications

References 0 publications

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Serum Parathyroid Hormone and Calcitonin Levels in Racehorses with Fracture.

Cosecretion of calcitonin and calcitonin gene-related peptide from cultured rat medullary thyroid C cells

Contact Info

Product

Resources

About

A Study of Thyrocalcitonin Secretion by Direct Measurement ofin VivoSecretion Rates in Pigs¹