A Study of Three Polymorphic Sites of ADA Gene in Colon Cancer

Abstract

Adenosine inhibits the immune response in tumors. Adenosine deaminase (ADA) controls adenosine level and as ecto-enzyme acts as costimulatory molecule of adenosine receptors and/or CD26. We examined ADA₁, ADA₂, ADA₆ polymorphic sites of ADA gene in 109 subjects with colon cancer from Rome's population and in 246 blood donors as controls from the same population. In colon cancer ADA₁*2/ADA₂*1 haplotype is more represented, while ADA₁*2/ADA₂*2 is less represented than in controls. ADA₂*2/ADA₆*2 is less represented in patients than in controls. Polymorphic sites of ADA might influence cell-mediated anti-tumor immune responses controlling adenosine level and extraenzymatic protein functions.

The effect of genetic and non genetic factors on the association of T1D with PTPN22 genotype and with the ACP1-ADA1 joint genotype

“…We have reexamined the data on 314 children with T1D and on 770 controls from the White population of Rome. Written informed consent was obtained by the subjects or by their mothers to participate to the study that was approved by I.R.B.. PTPN22, ADA 2 and p53 codon 72 genotypes were determined by DNA analysis as previously described [3,5,6] . Statistical analysis were carried out by commercial software (SPSS).…”

“…T1D has been also found associated with sex, maternal age at birth [2] , Adenosine Deaminase locus 2 (ADA 2 ) genotype [3] and p53 codon 72 genotype [4] . In this note we have studied the effect of these variables on the association of T1D with PTPN22 and with ACP 1 -ADA 1 joint genotype.…”

Genetic Variability within ADA Gene and Endometrium Cancer

“…Verbal informed consent was obtained from these women to participate to the study that was approved by I.R.B. Data on 109 subjects with colon cancer and on 246 blood donors reported in a previous paper [5] are also shown.…”

“…We have recently found an association between colon cancer and ADA genetic polymorphism [5]. We studied three polymorphic sites ADA 1 , ADA 2 and ADA 6 .…”

Mini Review From the Molecular Base to the Diagnostic Value of Adenosine Deaminase

“…Under physiologic conditions, concentrations of adenosine in extracellular fluid vary from 40-600 nM and downstream effects of ADA signaling include regulation of a variety of homeostatic and adaptive functions (25). However, under some pathologic conditions adenosine levels are elevated, since ADA activity varies in many diseases, particularly in those associated with the immune system such as rheumatoid arthritis (20), cancers (26), diabetes (27), fertility (28), coronary artery disease (29), autism (19), and obesity (30). In conclusion, recent investigations suggest that ADA could play a role in initiation or progression of some diseases.…”