A study of the uterine protein variations during the estrus cycle in the cow: A comparison with the serum proteins

Alavi-Shoushtari, Sayed Mortaza; Asri-Rezai, S.; Abshenas, Jalil

doi:10.1016/j.anireprosci.2005.10.012

Cited by 15 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The estrous cycle and pregnancy-dependent changes in the protein concentration of serum and serum exosomes as observed in our study are also noticed in cattle 25 . In cattle, the estrus (day 0) serum samples contain a lower total protein concentration than in the other phases of the cycle, due to the reduction in serum alpha1, gamma 1, and gamma 2 globulins 25 . The quantitative differences in the individual constituents of the serum proteins that might have led to the observed lower total serum and exosome-extracted proteins on day 10 of the cycle than on day 0 and day 15 of the cycle were not enumerated in our study.…”

Section: Discussionsupporting

confidence: 81%

Serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle

V.R.,

D.T.,

Tatu

et al. 2023

Preprint

View full text Add to dashboard Cite

Exosomes are membrane-bound extracellular vesicles (EVs) that carry proteins, nucleic acids, and lipids in their cargo from the cells of origin. They are detected in circulatory body fluids and are indicated to play significant roles during the establishment of pregnancy in humans and animals. This study aimed to isolate, quantify, profile, compare, and identify the elected differentially expressed serum exosome proteins in cycling and early-pregnant buffaloes. Serum exosomes were isolated from 0.5 mL serum of non-pregnant day 0, day 10, and day 15 of the cycle (n = 6), and early pregnancy days 15, day 30, and day 60 (n = 5) buffaloes by precipitation method. The proteins of serum exosomes were harvested by sonication, passing through Sephadex G25 spin columns, quantified, and analyzed by one-(1D) and two-dimensional (2D) SDS-PAGE gel electrophoresis. Selected differentially expressed proteins were analyzed by mass spectrometry and generated peptide mass (m)/charge (z) ratio searched against NCBI cattle and buffalo database to identify the protein. The results indicated serum exosome protein on cycle day 10 was significantly lower than the tested other days of the cycle and early pregnancy samples. The 1D electrophoresis did not reveal any difference but the 2D analysis revealed subtle differences. Mass spectrometry analysis of 9 up-regulated spots (8 pregnancy-specific and one cycle-specific) identified 19 proteins under 11 categories by database search. They were functionally related to mitosis, cell cycle regulation, morphogenesis, and regulation of several cellular and molecular pathways which are essential for conceptus development. Out of 19 proteins 17 were found functionally interconnected due to their common properties indicating their importance during the period of critical period of cycle and early pregnancy. In summary, serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle.

show abstract

Section: Discussionsupporting

confidence: 81%

Serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle

V.R.,

D.T.,

Tatu

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Our results had showed that proteins orchestration had significant differentiation in all phases of oestrous cycle. Alavi had found the same results (Alavi‐Shoushtari et al, 2006). Literature has found protein expression structurally in uterus for healthier fertilization and zygote implantation (Murphy & Ballejo, 1994).…”

Section: Discussionsupporting

confidence: 58%

Association of faecal volatiles and steroids with behavioural expression during oestrous cycle of sheep (Ovis aries)

Yaseen

Hussain

Hameed

2023

Reprod Domestic Animals

View full text Add to dashboard Cite

This study was investigated to determine the faecal volatiles, steroid hormones and their correlation with behavioural signs during oestrous cycle in sheep (Ovis aries). This experiment was monitored from pro‐oestrous to met‐oestrous phases for correlation of endocrine dependence biochemical constituents in faeces and blood to detection of oestrous biomarkers. Medroxyprogesterone acetate sponges were used for 8 days to bring uniformity of oestrus in sheep. Faeces were collected during different phases of cycle and subjected to determination of fatty acids, minerals, oestrogens and progesterone analysis. Likewise, blood samples were also obtained for enzymatic and no‐enzymatic antioxidants. The results revealed that faecal progesterone and oestrogen levels significantly increased during pro‐oestrous and oestrous phase, respectively (p < .05). The blood plasma enzymatic levels showed significant demarcation during the oestrous phase than other periods (p < .05). There were also reported marked variations in volatile fatty acids across different phases of the oestrous cycle. Interestingly, methyl octanoate, methyl cis‐10 pentadecenoate and methyl heptadecanoate volatiles were found to be specific to oestrus period. In addition, methyl hexanoate, methyl palmitoleate and methyl cis‐9 oleate were identified in met‐oestrus, indicating their possible role to be an oestrous biomarker. It is concluded that the pattern of volatile compounds and faecal steroids, in association with behavioural patterns, could be measured as a non‐invasive method of heat detection in sheep.

show abstract

“…Therefore, the effect of ULF collection on impoverishing the uterine luminal protein was probably disguised by the influx of blood proteins, such as albumin, that alone constitutes 35% to 50% of total serum protein [ 28 ]. While albumin is a normal component of uterine fluid in cattle [ 29 ], it is found in much lower concentrations in the uterine fluid (0.88 to 0.98 g/dL) than in serum samples (2.11 to 2.36 g/dL; Alavi-Shoushtari et al [ 30 ]). Contamination by plasma and interstitial fluid is a common issue when uterine flushings are performed in vivo, transcervically in cattle [ 29 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

Martins

Pugliesi

Sponchiado

et al. 2018

J Animal Sci Biotechnol

View full text Add to dashboard Cite

BackgroundA major, unresolved issue is how the uterine microenvironment determines pregnancy success in cattle. Before implantation, conceptus development depends on the uterine secretome (i.e., histotroph). Despite its pivotal role, little is known about the dynamics of histotroph synthesis and changes in composition throughout the early diestrus and the relevance to pregnancy establishment. We hypothesize that disturbances on histotroph composition affect the establishment of pregnancy. Aim was to disturb histotroph composition at early diestrus and verify the effects on: (Exp. 1) timing to restore its composition; and (Exp. 2) pregnancy rate after multiple-embryo transfer. Estrous cycle of multiparous Nelore cows were synchronized and estrus was considered d 0 (D0) of the experiments. Disturbance was through flushing each uterine horn with 30 mL of DMPBS and collecting the resulting uterine luminal flushing (ULF) on D1; D4; D7; D1 + D4 + D7. Control group remained not-collected. In Exp. 1, ULF was collected on D7.5 from all animals and used for quantification of total protein concentration and abundance of albumin. In Exp. 2, three in vitro-produced embryos were transferred to the uterine horn ipsilateral to the ovary containing the CL on D7.5 and pregnancy was checked on D25 by ultrasound.ResultsIn Exp. 1, ULF collection on D4 or D7 increased (1.5- to 2.2-folds) the total protein concentration and albumin abundance. ULF collection on D1 did not alter (P > 0.10) these endpoints. In Exp. 2, ULF collected on D4 or D7 decreased pregnancy rates to approximately half of that measured in the remaining groups.ConclusionsSubtle perturbations imposed to the native intrauterine milieu, such as those caused by a single, low-volume collection of ULF, profoundly disturbs intrauterine composition and pregnancy success. At least 4 d were necessary for the uterus to recover its composition and the functional capacity to carry post-implantation gestation.

show abstract

A study of the uterine protein variations during the estrus cycle in the cow: A comparison with the serum proteins

Cited by 15 publications

References 17 publications

Serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle

Serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle

Association of faecal volatiles and steroids with behavioural expression during oestrous cycle of sheep (Ovis aries)

Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

Contact Info

Product

Resources

About