Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

Martins, Thiago; Pugliesi, Guilherme; Sponchiado, Mariana; Gonella-Diaza, Ángela María; Ojeda-Rojas, Oscar Alejandro; Rodríguez, Fernando Mateos; Ramos, R. S.; Basso, Andrea; Binelli, Mário

doi:10.1186/s40104-018-0285-6

Cited by 21 publications

(23 citation statements)

References 39 publications

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“…Placement of a large volume of fluid (30 mL) can increase the accumulation of proteins in the uterine lumen and compromise embryo survival ( Martins et al, 2018b ). Whether a smaller volume of fluid such as that in a 0.25-mL ET straw also causes deleterious changes in the uterine fluid composition is unknown.…”

Section: Causes Of Pregnancy Loss Associated With Etmentioning

confidence: 99%

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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Typically, bovine embryos are transferred into recipient females about day 7 after estrus or anticipated ovulation, when the embryo has reached the blastocyst stage of development. All the biological and technical causes for failure of a female to produce a blastocyst 7 d after natural or artificial insemination (AI) are avoided when a blastocyst-stage embryo is transferred into the female. It is reasonable to expect, therefore, that pregnancy success would be higher for embryo transfer (ET) recipients than for inseminated females. This expectation is not usually met unless the recipient is exposed to heat stress or is classified as a repeat-breeder female. Rather, pregnancy success is generally similar for ET and AI. The implication is that either one or more of the technical aspects of ET have not yet been optimized or that underlying female fertility that causes an embryo to die before day 7 also causes it to die later in pregnancy. Improvements in pregnancy success after ET will depend upon making a better embryo, improving uterine receptivity, and forging new tools for production and transfer of embryos. Key to accelerating progress in improving pregnancy rates will be the identification of phenotypes or phenomes that allow the prediction of embryo competence for survival and maternal capacity to support embryonic development.

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Section: Causes Of Pregnancy Loss Associated With Etmentioning

confidence: 99%

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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“…Berkkanoglu et al (2006) reported no detrimental effect of uterine flushing immediately prior to embryo transfer in human. Martins et al (2018), however, showed that bovine uteri needed 4 days to recover from the flushing disruption to return to normal pregnancy rates.…”

Section: Sampling or Chemical Processingmentioning

confidence: 99%

Proteomics and metabolomics in cow fertility: a systematic review

et al. 2020

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Cow subfertility is a multi-factorial problem in many countries which is only starting to be unravelled. Molecular biology can provide a substantial source of insight into its causes and potential solutions, particularly through large scale, untargeted omics approaches. In this systematic review we set out to compile, assess and integrate the latest proteomic and metabolomic research on cow reproduction, specifically that on the female reproductive tract and early embryo. We herein report a general improvement in technical standards throughout the temporal span examined, however significant methodological limitations are also identified. We propose easily actionable avenues for ameliorating these shortcomings and enhancing the reach of this field. Text mining and pathway analysis corroborate the relevance of proteins and metabolites related to the triad oxidative stress-inflammation-disease on reproductive function. We envisage a breakthrough in cattle reproductive molecular research within the next few years as in vivo sample techniques are improved, omics analysis equipment becomes more affordable and widespread, and software tools for single- and multi-omics data processing are further developed. Additional investigation of the impact of local oxidative stress and inflammation on fertility, both at the local and systemic levels, is key towards realising the full potential of this field.

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“…The respective proteins have immunomodulatory and metabolic functions and regulate growth and angiogenesis [ 27 ]. Minor alterations in the secretome composition have been reported to impair early pregnancy in cattle [ 28 , 29 ]. IFNτ is also known to have immunosuppressive functions.…”

Section: Introductionmentioning

confidence: 99%

Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants

Schabmeyer¹,

Kneidl²,

Schneider³

et al. 2021

Animals

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The inadequate maternal recognition of embryonic interferon τ (IFNτ) might explain subfertility in cattle. This study aimed at modeling the inducibility of type 1 interferon receptor subunits 1/2 (IFNAR1/2), mimicking competition between IFNτ and infection-associated interferon α (IFNα), and simulating type 1 interferon pathways in vitro. Endometrial explants (n = 728 from n = 26 healthy uteri) were collected at the abattoir, challenged with IFNτ and/or IFNα in different concentrations, and incubated for 24 h. Gene expression analysis confirmed the inducibility of IFNAR1/2 within this model, it being most prominent in IFNAR2 with 10 ng/mL IFNα (p = 0.001). The upregulation of interferon-induced GTP-binding protein (MX1, classical pathway) was higher in explants treated with 300 ng/mL compared to 10 ng/mL IFNτ (p < 0.0001), whereas the non‑classical candidate fatty acid binding protein 3 (FABP3) exhibited significant downregulation comparing 300 ng/mL to 10 ng/mL IFNτ. The comparison of explants challenged with IFNτ + IFNα indicated the competition of IFNτ and IFNα downstream of the regulatory factors. In conclusion, using this well-defined explant model, interactions between infection-associated signals and IFNτ were indicated. This model can be applied to verify these findings and to mimic and explore the embryo–maternal contact zone in more detail.

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Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

Cited by 21 publications

References 39 publications

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Proteomics and metabolomics in cow fertility: a systematic review

Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants

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